ID   ALGJ_PSESM              Reviewed;         391 AA.
AC   Q887Q7;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Probable alginate O-acetylase AlgJ;
DE            EC=2.3.1.-;
DE   AltName: Full=Alginate biosynthesis protein AlgJ;
DE   Flags: Precursor;
GN   Name=algJ; OrderedLocusNames=PSPTO_1234;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: Together with AlgI and AlgF, forms an inner membrane complex
CC       which probably interacts with the alginate polymerization-transport
CC       complex and adds acetyl groups at the O-2 and O-3 positions of
CC       mannuronate residues. Acetylation of alginate is important for the
CC       architecture of biofilms and increases the ability of alginate to act
CC       as a defense barrier (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Peripheral
CC       membrane protein {ECO:0000250}; Periplasmic side {ECO:0000250}.
CC       Periplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AlgJ family. {ECO:0000305}.
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DR   EMBL; AE016853; AAO54759.1; -; Genomic_DNA.
DR   RefSeq; NP_791064.1; NC_004578.1.
DR   RefSeq; WP_011103480.1; NC_004578.1.
DR   AlphaFoldDB; Q887Q7; -.
DR   SMR; Q887Q7; -.
DR   STRING; 223283.PSPTO_1234; -.
DR   EnsemblBacteria; AAO54759; AAO54759; PSPTO_1234.
DR   GeneID; 1182870; -.
DR   KEGG; pst:PSPTO_1234; -.
DR   PATRIC; fig|223283.9.peg.1255; -.
DR   eggNOG; ENOG502Z851; Bacteria.
DR   HOGENOM; CLU_057510_0_0_6; -.
DR   OMA; FNEGRPG; -.
DR   OrthoDB; 1439364at2; -.
DR   PhylomeDB; Q887Q7; -.
DR   UniPathway; UPA00286; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd14442; AlgJ_like; 1.
DR   InterPro; IPR034657; AlgJ.
DR   InterPro; IPR031811; ALGX/ALGJ_SGNH-like.
DR   Pfam; PF16822; ALGX; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Alginate biosynthesis; Cell inner membrane; Cell membrane;
KW   Membrane; Periplasm; Reference proteome; Signal; Transferase.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..391
FT                   /note="Probable alginate O-acetylase AlgJ"
FT                   /id="PRO_0000001123"
FT   ACT_SITE        190
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        192
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        288
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   391 AA;  42964 MW;  0FAB645A65EE55A9 CRC64;
     MTRSLRILYI AIFLGILLIL GAWSLRSFSN FSTSAETTVL NGKWTKAAET HYDDEFPIKR
     LGTNLWAALD FKLFNEGRPG VVLGKDQWLY TDEEFDAVAN GEQNEADNLA LIQGVRDALE
     KQGSKLVLAI VPAKTRLYPE HIGDNKPASL HADLYQQFHA QVAKAGIFAP DLLAPLQAAK
     QQGQVFLRTD THWTPMGAEV AAQQLGAAIA QKAPLEGEPE QFVTQAIETA PYKGDLTTFL
     PLDPLFSNLL PKPDELQKRS TDPVAGEAAG GDALFADSDV AVGLVGTSYS ANPNWNFVGA
     LKQALRSDVV NYAEDGHGPI LPMLKYLQTD AFKNTPPQVV IWEFPERYLP AHNDLGEFDP
     KWIAELKKSR DTQENVALNA KPSESPNRAQ N