GAOX2_ARATH
ID GAOX2_ARATH Reviewed; 378 AA.
AC Q39111;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Gibberellin 20 oxidase 2 {ECO:0000305|PubMed:7630935};
DE EC=1.14.11.- {ECO:0000269|PubMed:7630935};
DE AltName: Full=GA 20-oxidase 2 {ECO:0000305|PubMed:7630935};
DE AltName: Full=Gibberellin C-20 oxidase 2;
GN Name=GA20OX2; Synonyms=20ox, At2353; OrderedLocusNames=At5g51810;
GN ORFNames=MIO24.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Landsberg erecta; TISSUE=Flower bud, and Stem;
RX PubMed=7630935; DOI=10.1104/pp.108.3.1049;
RA Phillips A.L., Ward D.A., Uknes S., Appleford N.E.J., Lange T.,
RA Huttly A.K., Gaskin P., Graebe J.E., Hedden P.;
RT "Isolation and expression of three gibberellin 20-oxidase cDNA clones from
RT Arabidopsis.";
RL Plant Physiol. 108:1049-1057(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP INDUCTION BY COLD.
RX PubMed=14729916; DOI=10.1105/tpc.018143;
RA Yamauchi Y., Ogawa M., Kuwahara A., Hanada A., Kamiya Y., Yamaguchi S.;
RT "Activation of gibberellin biosynthesis and response pathways by low
RT temperature during imbibition of Arabidopsis thaliana seeds.";
RL Plant Cell 16:367-378(2004).
RN [5]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=15927942; DOI=10.1093/pcp/pci141;
RA Kim Y.C., Nakajima M., Nakayama A., Yamaguchi I.;
RT "Contribution of gibberellins to the formation of Arabidopsis seed coat
RT through starch degradation.";
RL Plant Cell Physiol. 46:1317-1325(2005).
RN [6]
RP INDUCTION BY LIGHT.
RX PubMed=15923331; DOI=10.1104/pp.104.059055;
RA Hisamatsu T., King R.W., Helliwell C.A., Koshioka M.;
RT "The involvement of gibberellin 20-oxidase genes in phytochrome-regulated
RT petiole elongation of Arabidopsis.";
RL Plant Physiol. 138:1106-1116(2005).
RN [7]
RP INDUCTION BY AUXIN AND PACLOBUTRAZOL, AND TISSUE SPECIFICITY.
RX PubMed=16905669; DOI=10.1104/pp.106.084871;
RA Frigerio M., Alabadi D., Perez-Gomez J., Garcia-Carcel L., Phillips A.L.,
RA Hedden P., Blazquez M.A.;
RT "Transcriptional regulation of gibberellin metabolism genes by auxin
RT signaling in Arabidopsis.";
RL Plant Physiol. 142:553-563(2006).
RN [8]
RP FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND INDUCTION BY
RP GIBBERELLIN.
RC STRAIN=cv. Columbia;
RX PubMed=18069939; DOI=10.1111/j.1365-313x.2007.03356.x;
RA Rieu I., Ruiz-Rivero O., Fernandez-Garcia N., Griffiths J., Powers S.J.,
RA Gong F., Linhartova T., Eriksson S., Nilsson O., Thomas S.G.,
RA Phillips A.L., Hedden P.;
RT "The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially
RT redundantly, to promote growth and development throughout the Arabidopsis
RT life cycle.";
RL Plant J. 53:488-504(2008).
RN [9]
RP 3D-STRUCTURE MODELING, AND GENE FAMILY.
RX PubMed=21056641; DOI=10.1016/j.gene.2010.10.010;
RA Han F., Zhu B.;
RT "Evolutionary analysis of three gibberellin oxidase genes in rice,
RT Arabidopsis, and soybean.";
RL Gene 473:23-35(2011).
CC -!- FUNCTION: Key oxidase enzyme in the biosynthesis of gibberellin that
CC catalyzes the conversion of GA12 to GA9, via a three-step oxidation at
CC C-20 of the GA skeleton, and GA25 is also formed as a minor product.
CC GA53 is less effectively oxidized than GA12 and is only oxidized one
CC step to GA44 (PubMed:7630935). Involved in the promotion of the floral
CC transition, fertility and silique elongation, but plays only a minor
CC role in elongation of seedling organs. Acts redundantly with GA20OX1
CC (PubMed:18069939). {ECO:0000269|PubMed:18069939,
CC ECO:0000269|PubMed:7630935}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 2-oxoglutarate + gibberellin A12 + H(+) + 3 O2 = 3 CO2 +
CC gibberellin A9 + 2 H2O + 2 succinate; Xref=Rhea:RHEA:60772,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58627, ChEBI:CHEBI:73255;
CC Evidence={ECO:0000269|PubMed:7630935};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60773;
CC Evidence={ECO:0000269|PubMed:7630935};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + gibberellin A12 + O2 = CO2 + gibberellin A15
CC + succinate; Xref=Rhea:RHEA:60776, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58627, ChEBI:CHEBI:143956;
CC Evidence={ECO:0000269|PubMed:7630935};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60777;
CC Evidence={ECO:0000269|PubMed:7630935};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + gibberellin A15 + O2 = CO2 + gibberellin A24
CC + H2O + succinate; Xref=Rhea:RHEA:60780, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810,
CC ChEBI:CHEBI:30031, ChEBI:CHEBI:143956, ChEBI:CHEBI:143957;
CC Evidence={ECO:0000269|PubMed:7630935};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60781;
CC Evidence={ECO:0000269|PubMed:7630935};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + gibberellin A53 + O2 = CO2 + gibberellin A44
CC + succinate; Xref=Rhea:RHEA:60800, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:143954, ChEBI:CHEBI:143955;
CC Evidence={ECO:0000269|PubMed:7630935};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60801;
CC Evidence={ECO:0000269|PubMed:7630935};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 2-oxoglutarate + gibberellin A12 + 3 O2 = 3 CO2 +
CC gibberellin A25 + H(+) + H2O + 3 succinate; Xref=Rhea:RHEA:60768,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58627, ChEBI:CHEBI:143959;
CC Evidence={ECO:0000269|PubMed:7630935};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60769;
CC Evidence={ECO:0000269|PubMed:7630935};
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Note=Binds 1 Fe(2+) ion per subunit.;
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC -!- TISSUE SPECIFICITY: Expressed in inflorescence and developing siliques.
CC Detected in seeds, roots, cotyledons and leaves. In seeds, specifically
CC detected at the rim of the embryo and the outer integument.
CC {ECO:0000269|PubMed:15927942, ECO:0000269|PubMed:16905669,
CC ECO:0000269|PubMed:18069939, ECO:0000269|PubMed:7630935}.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing siliques 3-13 days after
CC pollination. {ECO:0000269|PubMed:15927942}.
CC -!- INDUCTION: Negatively controlled by the level of physiologically active
CC gibberellin. Up-regulated by auxin, paclobutrazol, long day exposure
CC and cold treatment. {ECO:0000269|PubMed:14729916,
CC ECO:0000269|PubMed:15923331, ECO:0000269|PubMed:16905669,
CC ECO:0000269|PubMed:18069939}.
CC -!- DISRUPTION PHENOTYPE: Slightly smaller than the wild type.
CC {ECO:0000269|PubMed:18069939}.
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. GA20OX subfamily. {ECO:0000305}.
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DR EMBL; X83380; CAA58294.1; -; mRNA.
DR EMBL; AB010074; BAB11250.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96129.1; -; Genomic_DNA.
DR RefSeq; NP_199994.1; NM_124560.4.
DR AlphaFoldDB; Q39111; -.
DR SMR; Q39111; -.
DR STRING; 3702.AT5G51810.1; -.
DR PaxDb; Q39111; -.
DR PRIDE; Q39111; -.
DR ProteomicsDB; 228896; -.
DR EnsemblPlants; AT5G51810.1; AT5G51810.1; AT5G51810.
DR GeneID; 835256; -.
DR Gramene; AT5G51810.1; AT5G51810.1; AT5G51810.
DR KEGG; ath:AT5G51810; -.
DR Araport; AT5G51810; -.
DR TAIR; locus:2165341; AT5G51810.
DR eggNOG; KOG0143; Eukaryota.
DR HOGENOM; CLU_010119_16_3_1; -.
DR InParanoid; Q39111; -.
DR OMA; NVRAVHY; -.
DR OrthoDB; 755305at2759; -.
DR PhylomeDB; Q39111; -.
DR BioCyc; ARA:AT5G51810-MON; -.
DR BioCyc; MetaCyc:AT5G51810-MON; -.
DR BRENDA; 1.14.11.12; 399.
DR UniPathway; UPA00390; -.
DR PRO; PR:Q39111; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q39111; baseline and differential.
DR Genevisible; Q39111; AT.
DR GO; GO:0051213; F:dioxygenase activity; IBA:GO_Central.
DR GO; GO:0103055; F:gibberelli A15, 2-oxoglutarate:oxygen oxidoreductase activity; IEA:RHEA.
DR GO; GO:0045544; F:gibberellin 20-oxidase activity; IDA:TAIR.
DR GO; GO:0103054; F:gibberellin A12, 2-oxoglutarate:oxygen oxidoreductase activity (gibberellin A15-forming); IEA:RHEA.
DR GO; GO:0103056; F:gibberellin A53, 2-oxoglutarate:oxygen oxidoreductase activity; IEA:RHEA.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; IBA:GO_Central.
DR GO; GO:0009686; P:gibberellin biosynthetic process; IDA:TAIR.
DR GO; GO:0009739; P:response to gibberellin; IEP:TAIR.
DR GO; GO:0009416; P:response to light stimulus; IBA:GO_Central.
DR GO; GO:0009639; P:response to red or far red light; IEP:TAIR.
DR GO; GO:0009826; P:unidimensional cell growth; IBA:GO_Central.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 1: Evidence at protein level;
KW Iron; Metal-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..378
FT /note="Gibberellin 20 oxidase 2"
FT /id="PRO_0000219515"
FT DOMAIN 220..320
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 311
FT /evidence="ECO:0000255"
FT BINDING 245
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 247
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 301
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 378 AA; 42937 MW; 0B5CF4E6FDBD2B74 CRC64;
MAILCTTTSP AEKEHEPKQD LEKDQTSPLI FNPSLLNLQS QIPNQFIWPD EEKPSIDIPE
LNVPFIDLSS QDSTLEAPRV IAEACTKHGF FLVVNHGVSE SLIADAHRLM ESFFDMPLAG
KQKAQRKPGE SCGYASSFTG RFSTKLPWKE TLSFQFSNDN SGSRTVQDYF SDTLGQEFEQ
FGKVYQDYCE AMSSLSLKIM ELLGLSLGVN RDYFRGFFEE NDSIMRLNHY PPCQTPDLTL
GTGPHCDPSS LTILHQDHVN GLQVFVDNQW QSIRPNPKAF VVNIGDTFMA LSNGIFKSCL
HRAVVNRESA RKSMAFFLCP KKDKVVKPPS DILEKMKTRK YPDFTWSMFL EFTQKHYRAD
VNTLDSFSNW VITNNNPI
