GAOX2_ORYSI
ID GAOX2_ORYSI Reviewed; 389 AA.
AC P0C5H5; Q6YLX3; Q7EC22; Q7GBH4; Q8RVF5; Q8S492;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=Gibberellin 20 oxidase 2;
DE EC=1.14.11.- {ECO:0000250|UniProtKB:O04705};
DE AltName: Full=GA 20-oxidase 2;
DE AltName: Full=Gibberellin C-20 oxidase 2;
DE AltName: Full=Os20ox2;
DE AltName: Full=Protein semidwarf-1;
GN Name=20ox2; Synonyms=C20ox2, GA20, Sd-1;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], POLYMORPHISM, AND VARIANTS SD-1
RP 100-ALA--ARG-102 AND 103-VAL--SER-389 DEL.
RC STRAIN=cv. Doongara, and cv. Kyeema;
RX PubMed=12077303; DOI=10.1073/pnas.132266399;
RA Spielmeyer W., Ellis M.H., Chandler P.M.;
RT "Semidwarf (sd-1), 'green revolution' rice, contains a defective
RT gibberellin 20-oxidase gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:9043-9048(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS SD-1 100-ALA--ARG-102 AND
RP 103-VAL--SER-389 DEL.
RC STRAIN=cv. Habataki, cv. IR24, and cv. Milyang 23;
RX PubMed=11939564; DOI=10.1093/dnares/9.1.11;
RA Monna L., Kitazawa N., Yoshino R., Suzuki J., Masuda H., Maehara Y.,
RA Tanji M., Sato M., Nasu S., Minobe Y.;
RT "Positional cloning of rice semidwarfing gene, sd-1: rice 'green revolution
RT gene' encodes a mutant enzyme involved in gibberellin synthesis.";
RL DNA Res. 9:11-17(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (SD-1).
RC STRAIN=cv. Milyang 23;
RA Yoon U.H., Eun M.Y., Kim Y.H., Lee J.S., Yun D.W., Kim H.I., Hahn J.H.;
RT "Map-based cloning of semidwarf gene, Sd-1, in rice.";
RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. IR64;
RA Septiningsih E.M., Thomson M.J., Trijatmiko K.R., Moeljopawiro S.,
RA McCouch S.R.;
RT "The rice plant height quantitative trait locus ph1.1 maps within the
RT gibberellin 20 oxidase locus on chromosome 1.";
RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=11961544; DOI=10.1038/416701a;
RA Sasaki A., Ashikari M., Ueguchi-Tanaka M., Itoh H., Nishimura A.,
RA Swapan D., Ishiyama K., Saito T., Kobayashi M., Khush G.S., Kitano H.,
RA Matsuoka M.;
RT "A mutant gibberellin-synthesis gene in rice.";
RL Nature 416:701-702(2002).
CC -!- FUNCTION: Key oxidase enzyme in the biosynthesis of gibberellin that
CC catalyzes the conversion of GA53 to GA20 via a three-step oxidation at
CC C-20 of the GA skeleton. {ECO:0000269|PubMed:11961544}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 2-oxoglutarate + gibberellin A12 + H(+) + 3 O2 = 3 CO2 +
CC gibberellin A9 + 2 H2O + 2 succinate; Xref=Rhea:RHEA:60772,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58627, ChEBI:CHEBI:73255;
CC Evidence={ECO:0000250|UniProtKB:O04705};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60773;
CC Evidence={ECO:0000250|UniProtKB:O04705};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 2-oxoglutarate + gibberellin A53 + H(+) + 3 O2 = 3 CO2 +
CC gibberellin A20 + 2 H2O + 2 succinate; Xref=Rhea:RHEA:60796,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58526, ChEBI:CHEBI:143954;
CC Evidence={ECO:0000250|UniProtKB:O04705};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60797;
CC Evidence={ECO:0000250|UniProtKB:O04705};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC -!- POLYMORPHISM: Sd-1 is the major semidwarfing allele extensively used in
CC modern rice cultivars. {ECO:0000303|PubMed:12077303}.
CC -!- BIOTECHNOLOGY: This gene, also known as the 'green revolution gene' has
CC been introduced by conventional breeding procedures into several
CC cultivars. It provides rice cultivars with short, thick culms, raises
CC the harvest index, improves lodging resistance and responsiveness to
CC nitrogen fertilizer, resulting high yields without affecting panicle
CC and grain quality.
CC -!- MISCELLANEOUS: A second gene, 20ox1, is preferentially expressed in the
CC reproductive organs and enables the flowers in sd1 plants to develop
CC and be fertilized normally.
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. GA20OX subfamily. {ECO:0000305}.
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DR EMBL; AY114310; AAM56041.1; -; mRNA.
DR EMBL; AF465256; AAL87950.1; -; Genomic_DNA.
DR EMBL; AY115558; AAN73383.1; -; Genomic_DNA.
DR AlphaFoldDB; P0C5H5; -.
DR SMR; P0C5H5; -.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 1: Evidence at protein level;
KW Iron; Metal-binding; Oxidoreductase.
FT CHAIN 1..389
FT /note="Gibberellin 20 oxidase 2"
FT /id="PRO_0000306090"
FT DOMAIN 224..324
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 315
FT /evidence="ECO:0000255"
FT BINDING 249
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 251
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 305
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT VARIANT 100..102
FT /note="EHG -> ARR (in strain: cv. Doongara, cv. Habataki,
FT cv. IR24 and cv. Milyang 23; allele sd-1; semidwarfing)"
FT VARIANT 103..389
FT /note="Missing (in strain: cv. Doongara, cv. Habataki, cv.
FT IR24 and cv. Milyang 23; allele sd-1; semidwarfing)"
FT /evidence="ECO:0000269|PubMed:11939564,
FT ECO:0000269|PubMed:12077303"
SQ SEQUENCE 389 AA; 42513 MW; 01B26E496DF2EAC1 CRC64;
MVAEHPTPPQ PHQPPPMDST AGSGIAAPAA AAVCDLRMEP KIPEPFVWPN GDARPASAAE
LDMPVVDVGV LRDGDAEGLR RAAAQVAAAC ATHGFFQVSE HGVDAALARA ALDGASDFFR
LPLAEKRRAR RVPGTVSGYT SAHADRFASK LPWKETLSFG FHDRAAAPVV ADYFSSTLGP
DFAPMGRVYQ KYCEEMKELS LTIMELLELS LGVERGYYRE FFADSSSIMR CNYYPPCPEP
ERTLGTGPHC DPTALTILLQ DDVGGLEVLV DGEWRPVSPV PGAMVINIGD TFMALSNGRY
KSCLHRAVVN QRRERRSLAF FLCPREDRVV RPPPSAATPQ HYPDFTWADL MRFTQRHYRA
DTRTLDAFTR WLAPPAADAA ATAQVEAAS
