ID   ALGK_AZOVI              Reviewed;         460 AA.
AC   P94202;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Alginate biosynthesis protein AlgK;
DE   Flags: Precursor;
GN   Name=algK;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 9046;
RX   PubMed=9368366; DOI=10.1111/j.1574-6968.1997.tb12712.x;
RA   Mejia-Ruiz H., Moreno S., Guzman J., Najera R., Leon R., Soberon-Chavez G.,
RA   Espin G.;
RT   "Isolation and characterization of an Azotobacter vinelandii algK mutant.";
RL   FEMS Microbiol. Lett. 156:101-106(1997).
CC   -!- FUNCTION: May be involved in the polymerization of mannuronate to
CC       alginate.
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}; Periplasmic side {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Cells do not encyst.
CC       {ECO:0000269|PubMed:9368366}.
CC   -!- SIMILARITY: Belongs to the AlgK family. {ECO:0000305}.
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DR   EMBL; X98863; CAA67370.1; -; Genomic_DNA.
DR   AlphaFoldDB; P94202; -.
DR   SMR; P94202; -.
DR   UniPathway; UPA00286; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR006597; Sel1-like.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   SMART; SM00671; SEL1; 4.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Alginate biosynthesis; Cell outer membrane; Lipoprotein; Membrane;
KW   Palmitate; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           18..460
FT                   /note="Alginate biosynthesis protein AlgK"
FT                   /id="PRO_0000020666"
FT   LIPID           18
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           18
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   460 AA;  50987 MW;  467E5BC188E77921 CRC64;
     MNLTKPLLLS ALAGLTACAN LDLPDQRLAK EALQRGDTQT AERHFRQLAD MGFTEAQLGL
     ADMQLASGDP EQLRKAEQTY RMALDASPRA KARLGKLLAY KPTSSEAEKR EAAQLLSDAF
     AAGEDGVLLP LAMLYLKNPQ TFPDVSLQQR IDQWRAAGHP QADIAQIVVY RTQGTYDQHL
     DDIERICQQR LAEHSDCYVE LATVYLQARP EWTRTTACRA WCNSSWPLMA AHRAGGVSAQ
     LVTEVAGVLS NPLLGQSNEK TAQTMLEEIA PPPGSVWRVL IYDFPGTGDT DQMLDYLAWP
     CAAQPAADLL LGRLYYEGKL LPQDPFKAEE YFIKARATEN SAHYYLGQIY RRGFLGEVYP
     QKAVDSLLTA ARGGQASADY ALAQLYSQGR GIRIDLANAY VFARLAVLQG RPDSEPLLQE
     IKANLAPAER TRGEQMLHAE QQARYGVWQT STQLQAMQNQ