GAOXL_ARATH
ID GAOXL_ARATH Reviewed; 325 AA.
AC F4KBY0; Q9FGV8;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Gibberellin 20-oxidase-like protein {ECO:0000312|EMBL:BAB10331.1};
DE EC=1.14.11.- {ECO:0000255|PROSITE-ProRule:PRU00805};
GN OrderedLocusNames=At5g51310 {ECO:0000312|Araport:AT5G51310};
GN ORFNames=MWD22.26 {ECO:0000312|EMBL:BAB10331.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, INDUCTION BY PHOSPHATE DEFICIENCY, AND DISRUPTION PHENOTYPE.
RX PubMed=21248074; DOI=10.1104/pp.110.166520;
RA Lin W.D., Liao Y.Y., Yang T.J., Pan C.Y., Buckhout T.J., Schmidt W.;
RT "Coexpression-based clustering of Arabidopsis root genes predicts
RT functional modules in early phosphate deficiency signaling.";
RL Plant Physiol. 155:1383-1402(2011).
CC -!- FUNCTION: Negative regulator of root hair growth.
CC {ECO:0000305|PubMed:21248074}.
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00805};
CC -!- TISSUE SPECIFICITY: Highly expressed in elongation zone of lateral
CC roots. {ECO:0000305|PubMed:21248074}.
CC -!- INDUCTION: Down-regulated upon phosphate deficiency.
CC {ECO:0000305|PubMed:21248074}.
CC -!- DISRUPTION PHENOTYPE: Longer root hairs under Pi-deficient conditions.
CC {ECO:0000269|PubMed:21248074}.
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. GA20OX subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10331.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB023044; BAB10331.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AB025621; BAB10331.1; JOINED; Genomic_DNA.
DR EMBL; CP002688; AED96066.1; -; Genomic_DNA.
DR RefSeq; NP_199944.2; NM_124510.3.
DR AlphaFoldDB; F4KBY0; -.
DR SMR; F4KBY0; -.
DR STRING; 3702.AT5G51310.1; -.
DR PaxDb; F4KBY0; -.
DR PRIDE; F4KBY0; -.
DR ProteomicsDB; 248480; -.
DR EnsemblPlants; AT5G51310.1; AT5G51310.1; AT5G51310.
DR GeneID; 835205; -.
DR Gramene; AT5G51310.1; AT5G51310.1; AT5G51310.
DR KEGG; ath:AT5G51310; -.
DR Araport; AT5G51310; -.
DR TAIR; locus:2176237; AT5G51310.
DR eggNOG; KOG0143; Eukaryota.
DR HOGENOM; CLU_010119_6_3_1; -.
DR InParanoid; F4KBY0; -.
DR OMA; ISPCEDT; -.
DR OrthoDB; 755305at2759; -.
DR PRO; PR:F4KBY0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KBY0; baseline and differential.
DR GO; GO:0051213; F:dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0048767; P:root hair elongation; IMP:TAIR.
DR Gene3D; 2.60.120.330; -; 1.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Dioxygenase; Iron; Metal-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..325
FT /note="Gibberellin 20-oxidase-like protein"
FT /id="PRO_0000438345"
FT DOMAIN 152..266
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 186
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 188
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 244
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 257
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 325 AA; 37125 MW; 9870E4E34682497A CRC64;
MSLELPVFDI SKPLSESSLT SLQDACKEWG FFYVTNHGVS RDMYKKLRRF STGVFELEDE
EKMKMGASNY TPRFIASPFF ESLRVSGPDF YASAKSSVDA FSDQATDEEF SGLMKEYGEK
MTKLCEKIMK AILSSFGDDL HHKYYESEFG NCHGYFRINN YTIPSDQEDD HHNGDEQDLI
EGLGMHTDMS CITIVDQDDI GGLQVRTRDG IGLMDINPKD EALVVNVGDL LHAWTNGRLR
SSQHRVILKR RGFVGNRFSL AFFWCFDDGK VVFAPDEVVG GCEGMRVFRS FKCGDYLRFR
ESNEKGKFEK VGDTVEDFAR IEDRR
