ID   GAP1_SCHPO              Reviewed;         766 AA.
AC   P33277;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=GTPase-activating protein;
DE   AltName: Full=Ras GTPase-activating protein;
GN   Name=gap1; Synonyms=sar1, src1; ORFNames=SPBC646.12c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2038319; DOI=10.1128/mcb.11.6.3088-3094.1991;
RA   Imai Y., Miyake S., Hughes D.A., Yamamoto M.;
RT   "Identification of a GTPase-activating protein homolog in
RT   Schizosaccharomyces pombe.";
RL   Mol. Cell. Biol. 11:3088-3094(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1883874; DOI=10.1091/mbc.2.6.453;
RA   Wang Y., Boguski M.S., Riggs M., Rodgers L., Wigler M.;
RT   "sar1, a gene from Schizosaccharomyces pombe encoding a protein that
RT   regulates ras1.";
RL   Cell Regul. 2:453-465(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Inhibitory regulator of the Ras-cyclic AMP pathway.
CC       Stimulates the GTPase activity of Ras1.
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DR   EMBL; D10457; BAA01251.1; -; Genomic_DNA.
DR   EMBL; S37449; AAB19697.1; -; Genomic_DNA.
DR   EMBL; CU329671; CAA22816.1; -; Genomic_DNA.
DR   PIR; A40258; A40258.
DR   RefSeq; NP_595370.1; NM_001021278.2.
DR   AlphaFoldDB; P33277; -.
DR   SMR; P33277; -.
DR   BioGRID; 276968; 9.
DR   STRING; 4896.SPBC646.12c.1; -.
DR   iPTMnet; P33277; -.
DR   MaxQB; P33277; -.
DR   PaxDb; P33277; -.
DR   PRIDE; P33277; -.
DR   EnsemblFungi; SPBC646.12c.1; SPBC646.12c.1:pep; SPBC646.12c.
DR   PomBase; SPBC646.12c; gap1.
DR   VEuPathDB; FungiDB:SPBC646.12c; -.
DR   eggNOG; KOG2128; Eukaryota.
DR   HOGENOM; CLU_009455_0_0_1; -.
DR   InParanoid; P33277; -.
DR   OMA; TRQNTMS; -.
DR   PhylomeDB; P33277; -.
DR   PRO; PR:P33277; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005938; C:cell cortex; IBA:GO_Central.
DR   GO; GO:0051285; C:cell cortex of cell tip; IDA:PomBase.
DR   GO; GO:0071944; C:cell periphery; IDA:PomBase.
DR   GO; GO:0090726; C:cortical dynamic polarity patch; IDA:PomBase.
DR   GO; GO:0005829; C:cytosol; IDA:PomBase.
DR   GO; GO:0000935; C:division septum; IDA:PomBase.
DR   GO; GO:0005937; C:mating projection; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IDA:PomBase.
DR   GO; GO:0005096; F:GTPase activator activity; IDA:PomBase.
DR   GO; GO:0046580; P:negative regulation of Ras protein signal transduction; IMP:PomBase.
DR   GO; GO:1902917; P:positive regulation of mating projection assembly; IMP:PomBase.
DR   GO; GO:0032956; P:regulation of actin cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0043087; P:regulation of GTPase activity; IEA:InterPro.
DR   GO; GO:0032005; P:signal transduction involved in positive regulation of conjugation with cellular fusion; IMP:PomBase.
DR   Gene3D; 1.10.506.10; -; 1.
DR   InterPro; IPR000593; RasGAP_C.
DR   InterPro; IPR023152; RasGAP_CS.
DR   InterPro; IPR001936; RasGAP_dom.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   Pfam; PF00616; RasGAP; 1.
DR   Pfam; PF03836; RasGAP_C; 1.
DR   SMART; SM00323; RasGAP; 1.
DR   SUPFAM; SSF48350; SSF48350; 1.
DR   PROSITE; PS00509; RAS_GTPASE_ACTIV_1; 1.
DR   PROSITE; PS50018; RAS_GTPASE_ACTIV_2; 1.
PE   4: Predicted;
KW   GTPase activation; Reference proteome.
FT   CHAIN           1..766
FT                   /note="GTPase-activating protein"
FT                   /id="PRO_0000056660"
FT   DOMAIN          167..379
FT                   /note="Ras-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00167"
SQ   SEQUENCE   766 AA;  87540 MW;  7CAD917C76D39E58 CRC64;
     MTKRHSGTLS SSVLPQTNRL SLLRNRESTS VLYTIDLDME SDVEDAFFHL DRELHDLKQQ
     ISSQSKQNFV LERDVRYLDS KIALLIQNRM AQEEQHEFAK RLNDNYNAVK GSFPDDRKLQ
     LYGALFFLLQ SEPAYIASLV RRVKLFNMDA LLQIVMFNIY GNQYESREEH LLLSLFQMVL
     TTEFEATSDV LSLLRANTPV SRMLTTYTRR GPGQAYLRSI LYQCINDVAI HPDLQLDIHP
     LSVYRYLVNT GQLSPSEDDN LLTNEEVSEF PAVKNAIQER SAQLLLLTKR FLDAVLNSID
     EIPYGIRWVC KLIRNLTNRL FPSISDSTIC SLIGGFFFLR FVNPAIISPQ TSMLLDSCPS
     DNVRKTLATI AKIIQSVANG TSSTKTHLDV SFQPMLKEYE EKVHNLLRKL GNVGDFFEAL
     ELDQYIALSK KSLALEMTVN EIYLTHEIIL ENLDNLYDPD SHVHLILQEL GEPCKSVPQE
     DNCLVTLPLY NRWDSSIPDL KQNLKVTRED ILYVDAKTLF IQLLRLLPSG HPATRVPLDL
     PLIADSVSSL KSMSLMKKGI RAIELLDELS TLRLVDKENR YEPLTSEVEK EFIDLDALYE
     RIRAERDALQ DVHRAICDHN EYLQTQLQIY GSYLNNARSQ IKPSHSDSKG FSRGVGVVGI
     KPKNIKSSNT VKLSSQQLKK ESVLLNCTIP EFNVSNTYFT FSSPSTDNFV IAVYQRGHSK
     VLVEVCICLD DVLQRRYASN PVVDLGFLTF EANKLYHLFE QLFLRK