GAP_MYCS2
ID GAP_MYCS2 Reviewed; 272 AA.
AC Q3L890; A0QPH8;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Peptidoglycolipid exporter Gap {ECO:0000305};
DE AltName: Full=GPLs addressing protein {ECO:0000303|PubMed:16194230};
GN Name=gap {ECO:0000303|PubMed:16194230};
GN OrderedLocusNames=MSMEG_0403 {ECO:0000312|EMBL:ABK71066.1},
GN MSMEI_0393 {ECO:0000312|EMBL:AFP36874.1};
GN ORFNames=LJ00_02005 {ECO:0000312|EMBL:AIU05677.1};
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN TRANSPORT, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=16194230; DOI=10.1111/j.1365-2958.2005.04847.x;
RA Sonden B., Kocincova D., Deshayes C., Euphrasie D., Rhayat L., Laval F.,
RA Frehel C., Daffe M., Etienne G., Reyrat J.M.;
RT "Gap, a mycobacterial specific integral membrane protein, is required for
RT glycolipid transport to the cell surface.";
RL Mol. Microbiol. 58:426-440(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=25657281; DOI=10.1128/genomea.01520-14;
RA Mohan A., Padiadpu J., Baloni P., Chandra N.;
RT "Complete genome sequences of a Mycobacterium smegmatis laboratory strain
RT (MC2 155) and isoniazid-resistant (4XR1/R2) mutant strains.";
RL Genome Announc. 3:E01520-E01520(2015).
CC -!- FUNCTION: Required for the transport of peptidoglycolipids (GPLs) to
CC the cell surface. {ECO:0000269|PubMed:16194230}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:16194230}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant synthesizes GPL at a level
CC similar to the wild-type strain, but is unable to transport it to the
CC cell surface. GPLs are retained within the cytoplasmic compartment.
CC Mutant is not able to slide. {ECO:0000269|PubMed:16194230}.
CC -!- SIMILARITY: Belongs to the peptidoglycolipid addressing protein (GAP)
CC (TC 2.A.116) family. {ECO:0000305}.
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DR EMBL; AY439015; AAT01808.1; -; Genomic_DNA.
DR EMBL; CP000480; ABK71066.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP36874.1; -; Genomic_DNA.
DR EMBL; CP009494; AIU05677.1; -; Genomic_DNA.
DR RefSeq; WP_011726918.1; NZ_SIJM01000040.1.
DR RefSeq; YP_884816.1; NC_008596.1.
DR AlphaFoldDB; Q3L890; -.
DR STRING; 246196.MSMEI_0393; -.
DR TCDB; 2.A.116.1.1; the peptidoglycolipid addressing protein (gap) family.
DR EnsemblBacteria; ABK71066; ABK71066; MSMEG_0403.
DR EnsemblBacteria; AFP36874; AFP36874; MSMEI_0393.
DR GeneID; 66738589; -.
DR KEGG; msb:LJ00_02005; -.
DR KEGG; msg:MSMEI_0393; -.
DR KEGG; msm:MSMEG_0403; -.
DR PATRIC; fig|246196.19.peg.399; -.
DR eggNOG; ENOG5031F2T; Bacteria.
DR HOGENOM; CLU_068662_1_0_11; -.
DR OMA; GKGAWEN; -.
DR OrthoDB; 1336990at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR InterPro; IPR021315; Gap/Sap.
DR Pfam; PF11139; SfLAP; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Lipid transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..272
FT /note="Peptidoglycolipid exporter Gap"
FT /id="PRO_0000432807"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 272 AA; 29535 MW; 9A70315E9CB7B51F CRC64;
MWSDILGLAL FVSLNPLLLG FILLVLSRPR PVPNLVVFWV GCLIVNVPGF LIPLFVLRAV
PSFAEFAEDL TTADPSSGIE PFQLGTGIFA LAVSAVIALR MWVKRRANQP VLVGSGAGDR
GPTDDASTLV LDSGAREARE PGAIARMILR MRSALQRLVS RLHQEWENGA LWVALVFGLA
YIPPPPLVLL VDTIIGGSGA PIGTQIIAVF VFIMAMLAVF EITLLSYVIA PRRTQAVLEP
LHEWSHRHRQ MILLVLFGAV GIWELIVGLG VI
