GAR1A_MOUSE
ID GAR1A_MOUSE Reviewed; 297 AA.
AC B2RXB0;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Golgi associated RAB2 interactor protein 1A {ECO:0000305};
GN Name=Garin1a;
GN Synonyms=Fam137b, Fam71f2, GARI {ECO:0000303|PubMed:18256213};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:AAI51116.1};
RN [1] {ECO:0000312|EMBL:BAH03389.1}
RP NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH RAB2B, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=BALB/cJ {ECO:0000312|EMBL:BAH03389.1};
RX PubMed=18256213; DOI=10.1074/mcp.m700569-mcp200;
RA Fukuda M., Kanno E., Ishibashi K., Itoh T.;
RT "Large scale screening for novel rab effectors reveals unexpected broad Rab
RT binding specificity.";
RL Mol. Cell. Proteomics 7:1031-1042(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3] {ECO:0000312|EMBL:AAI51116.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain {ECO:0000312|EMBL:AAI51116.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=34714330; DOI=10.1242/dev.199644;
RA Morohoshi A., Miyata H., Oyama Y., Oura S., Noda T., Ikawa M.;
RT "FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation
RT and male fertility in mice.";
RL Development 148:0-0(2021).
CC -!- FUNCTION: RAB2B effector protein required for accurate acrosome
CC formation and normal male fertility. {ECO:0000269|PubMed:34714330}.
CC -!- SUBUNIT: Interacts (via N-terminus) with RAB2B (in GTP-bound form).
CC {ECO:0000269|PubMed:18256213}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000269|PubMed:18256213}.
CC -!- TISSUE SPECIFICITY: Expressed in testis (at protein level).
CC {ECO:0000269|PubMed:34714330}.
CC -!- DEVELOPMENTAL STAGE: Expressed from day 21, around when spermiogenesis
CC occurs (PubMed:34714330). Expression dramatically increases at the mid-
CC round spermatid stage (steps 4-6) (PubMed:34714330).
CC {ECO:0000269|PubMed:34714330}.
CC -!- DISRUPTION PHENOTYPE: Mutant male fertility is significantly impaired
CC compared with that of the controls (PubMed:34714330). Spermatozoa from
CC mutant mice showed abnormal head shapes with an abnormal acrosome
CC morphology (PubMed:34714330). {ECO:0000269|PubMed:34714330}.
CC -!- SIMILARITY: Belongs to the GARIN family. {ECO:0000305}.
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DR EMBL; AB473724; BAH03389.1; -; mRNA.
DR EMBL; BC151115; AAI51116.1; -; mRNA.
DR EMBL; BC151117; AAI51118.1; -; mRNA.
DR RefSeq; NP_001094956.1; NM_001101486.1.
DR AlphaFoldDB; B2RXB0; -.
DR STRING; 10090.ENSMUSP00000110941; -.
DR PhosphoSitePlus; B2RXB0; -.
DR PaxDb; B2RXB0; -.
DR PRIDE; B2RXB0; -.
DR ProteomicsDB; 337178; -.
DR Antibodypedia; 9588; 42 antibodies from 14 providers.
DR Ensembl; ENSMUST00000115286; ENSMUSP00000110941; ENSMUSG00000079652.
DR GeneID; 245884; -.
DR KEGG; mmu:245884; -.
DR UCSC; uc009bde.1; mouse.
DR CTD; 346653; -.
DR MGI; MGI:2141439; Fam71f2.
DR VEuPathDB; HostDB:ENSMUSG00000079652; -.
DR eggNOG; ENOG502S7XV; Eukaryota.
DR GeneTree; ENSGT00940000162027; -.
DR HOGENOM; CLU_069391_1_0_1; -.
DR InParanoid; B2RXB0; -.
DR OMA; RIEFTHC; -.
DR OrthoDB; 1360447at2759; -.
DR PhylomeDB; B2RXB0; -.
DR TreeFam; TF336050; -.
DR BioGRID-ORCS; 245884; 1 hit in 72 CRISPR screens.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; B2RXB0; protein.
DR Bgee; ENSMUSG00000079652; Expressed in spermatid and 46 other tissues.
DR ExpressionAtlas; B2RXB0; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0001675; P:acrosome assembly; IMP:UniProtKB.
DR InterPro; IPR022168; DUF3699.
DR Pfam; PF12480; DUF3699; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Reference proteome.
FT CHAIN 1..297
FT /note="Golgi associated RAB2 interactor protein 1A"
FT /id="PRO_0000455123"
FT REGION 226..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..257
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 297 AA; 32845 MW; BEF5EF8B754AEF73 CRC64;
MSKIRGLPPA IRDPGPGVEL GVVDGLLCQL IHSPEFNLFS DSVVFESTFI QVTKQGNWMD
AYERSATMIL GVTSSVPSLP LPNILLMANV TWPHGPFSTC STLGAPVITL SRILPLKYVE
LQIYDRTQRI LRVRTVTEKI YYLRLHEKHP QAVFQFWIRL VKILQKGLSI TTKDPRIQFT
HCLVPKMSNS STETATESSL PASSQNSEAI MLLAAERNSS SLLELSNRHQ TSRDRHTDTA
TETDNSGNCK STPLVASSAS MPMRAALTHS LWEQEDSNEN FLQAPVASSL GENLLGP
