GAR1B_MOUSE
ID GAR1B_MOUSE Reviewed; 341 AA.
AC Q3UZD7; B2RW23;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Golgi associated RAB2 interactor protein 1B {ECO:0000305};
DE AltName: Full=Golgi associated RAB2 interactor-like 1 {ECO:0000303|PubMed:34714330};
DE Short=GARI-L1 {ECO:0000303|PubMed:34714330};
GN Name=Garin1b;
GN Synonyms=Fam137a, Fam71f1, Garil1 {ECO:0000303|PubMed:34714330};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RAB2A AND RAB2B, TISSUE
RP SPECIFICITY, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX PubMed=34714330; DOI=10.1242/dev.199644;
RA Morohoshi A., Miyata H., Oyama Y., Oura S., Noda T., Ikawa M.;
RT "FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation
RT and male fertility in mice.";
RL Development 148:0-0(2021).
CC -!- FUNCTION: RAB2B effector protein required for accurate acrosome
CC formation and normal male fertility (PubMed:34714330). In complex with
CC RAB2A/RAB2B, seems to suppress excessive vesicle trafficking during
CC acrosome formation (PubMed:34714330). {ECO:0000269|PubMed:34714330}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000269|PubMed:34714330}.
CC -!- TISSUE SPECIFICITY: Expressed in testis (at protein level).
CC {ECO:0000269|PubMed:34714330}.
CC -!- DEVELOPMENTAL STAGE: Expressed from day 21, around when spermiogenesis
CC occurs (PubMed:34714330). Expression dramatically increases at the mid-
CC round spermatid stage (steps 4-6) (PubMed:34714330).
CC {ECO:0000269|PubMed:34714330}.
CC -!- DISRUPTION PHENOTYPE: Mutant males are sterile (PubMed:34714330).
CC Spermatozoa from mutant mice showed abnormal head shapes with an
CC abnormal swollen acrosome morphology and impaired acrosome reaction
CC (PubMed:34714330). {ECO:0000269|PubMed:34714330}.
CC -!- SIMILARITY: Belongs to the GARIN family. {ECO:0000305}.
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DR EMBL; AK133908; BAE21920.1; -; mRNA.
DR EMBL; BC147513; AAI47514.1; -; mRNA.
DR CCDS; CCDS39451.1; -.
DR RefSeq; NP_001276592.1; NM_001289663.1.
DR RefSeq; NP_001276593.1; NM_001289664.1.
DR RefSeq; NP_001276594.1; NM_001289665.1.
DR RefSeq; NP_997141.2; NM_207258.3.
DR RefSeq; XP_017177142.1; XM_017321653.1.
DR AlphaFoldDB; Q3UZD7; -.
DR STRING; 10090.ENSMUSP00000087973; -.
DR PhosphoSitePlus; Q3UZD7; -.
DR PaxDb; Q3UZD7; -.
DR PRIDE; Q3UZD7; -.
DR ProteomicsDB; 271839; -.
DR Antibodypedia; 17801; 39 antibodies from 11 providers.
DR DNASU; 330277; -.
DR Ensembl; ENSMUST00000090487; ENSMUSP00000087973; ENSMUSG00000039742.
DR GeneID; 330277; -.
DR KEGG; mmu:330277; -.
DR UCSC; uc009bdf.1; mouse.
DR CTD; 84691; -.
DR MGI; MGI:3032524; Fam71f1.
DR VEuPathDB; HostDB:ENSMUSG00000039742; -.
DR eggNOG; ENOG502S7XV; Eukaryota.
DR GeneTree; ENSGT00940000161477; -.
DR HOGENOM; CLU_069391_0_0_1; -.
DR InParanoid; Q3UZD7; -.
DR TreeFam; TF336050; -.
DR BioGRID-ORCS; 330277; 3 hits in 73 CRISPR screens.
DR PRO; PR:Q3UZD7; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q3UZD7; protein.
DR Bgee; ENSMUSG00000039742; Expressed in seminiferous tubule of testis and 17 other tissues.
DR ExpressionAtlas; Q3UZD7; baseline and differential.
DR Genevisible; Q3UZD7; MM.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0001675; P:acrosome assembly; IMP:UniProtKB.
DR GO; GO:0007340; P:acrosome reaction; IMP:UniProtKB.
DR InterPro; IPR022168; DUF3699.
DR Pfam; PF12480; DUF3699; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Reference proteome.
FT CHAIN 1..341
FT /note="Golgi associated RAB2 interactor protein 1B"
FT /id="PRO_0000311687"
SQ SEQUENCE 341 AA; 38687 MW; 217E10A0E390EA81 CRC64;
MMTSVPPRKS WWTSKKTVKV IRSYPTFPSL NAWEKFRGLL PVDGETNPGV GLGVEEGLLC
QMVHSPEFNL FPNSVVFESN FVQVRRSRNW KEIYKASNTM ALGVTSSVPC LPLPNILLMA
RVKWHQGQSQ TWNRPSRAPS INLKSILPLK FVELQIWDDQ ERVLRLRTVT EKIYYLKLHP
DHPETVFHFW IRLVQILHKG LSITTKDPTI LVTHCLVPKS LCSPGGKTEL VQKKSKGLQP
SESLTHLMAQ GESETLSQIF SDLHQQKQYR SEKMHINKTS SEKATPCEDS IPCTCDLNWR
DAFMFGEWER ENPSGPQPLS LLGTLAASSR PRLSLTGGNS I
