GAR2_SCHPO
ID GAR2_SCHPO Reviewed; 500 AA.
AC P41891; O13707;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Protein gar2;
GN Name=gar2; ORFNames=SPAC140.02;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=7596817; DOI=10.1093/nar/23.11.1912;
RA Gulli M.-P., Girard J.-P., Zabetakis D., Lapeyre B., Melese T.,
RA Caizergues-Ferrer M.;
RT "gar2 is a nucleolar protein from Schizosaccharomyces pombe required for
RT 18S rRNA and 40S ribosomal subunit accumulation.";
RL Nucleic Acids Res. 23:1912-1918(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143; SER-144 AND SER-146, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Helps the assembly of pre-ribosomal particles containing 18S
CC rRNA.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus.
CC -!- SIMILARITY: Belongs to the RRM GAR family. {ECO:0000305}.
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DR EMBL; Z48166; CAA88179.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB86413.1; -; Genomic_DNA.
DR PIR; S55785; S55785.
DR RefSeq; NP_593531.1; NM_001018965.2.
DR AlphaFoldDB; P41891; -.
DR SMR; P41891; -.
DR BioGRID; 279314; 65.
DR STRING; 4896.SPAC140.02.1; -.
DR iPTMnet; P41891; -.
DR MaxQB; P41891; -.
DR PaxDb; P41891; -.
DR PRIDE; P41891; -.
DR EnsemblFungi; SPAC140.02.1; SPAC140.02.1:pep; SPAC140.02.
DR GeneID; 2542869; -.
DR KEGG; spo:SPAC140.02; -.
DR PomBase; SPAC140.02; gar2.
DR VEuPathDB; FungiDB:SPAC140.02; -.
DR eggNOG; KOG4210; Eukaryota.
DR HOGENOM; CLU_026791_1_1_1; -.
DR InParanoid; P41891; -.
DR OMA; GYVQYSS; -.
DR PRO; PR:P41891; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0001651; C:dense fibrillar component; IDA:PomBase.
DR GO; GO:0005730; C:nucleolus; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005732; C:sno(s)RNA-containing ribonucleoprotein complex; ISS:PomBase.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IBA:GO_Central.
DR GO; GO:0006364; P:rRNA processing; IMP:PomBase.
DR CDD; cd12447; RRM1_gar2; 1.
DR CDD; cd12448; RRM2_gar2; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR034272; Gar2_RRM1.
DR InterPro; IPR034276; Gar2_RRM2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50102; RRM; 2.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribosome biogenesis;
KW RNA-binding; rRNA processing.
FT CHAIN 1..500
FT /note="Protein gar2"
FT /id="PRO_0000081595"
FT DOMAIN 263..341
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 366..443
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 1..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 441..500
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..70
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 89..121
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..141
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 205..222
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..254
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 143
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 144
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 146
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT CONFLICT 339
FT /note="S -> P (in Ref. 1; CAA88179)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 500 AA; 52988 MW; 9D37FAD0C5161A0B CRC64;
MAKKDKTSVK KSVKETASKK GAIEKPSKSK KITKEAAKEI AKQSSKTDVS PKKSKKEAKR
ASSPEPSKKS VKKQKKSKKK EESSSESESE SSSSESESSS SESESSSSES ESSSSESSSS
ESEEEVIVKT EEKKESSSES SSSSESEEEE EAVVKIEEKK ESSSDSSSES SSSESESESS
SSESEEEEEV VEKTEEKKEG SSESSSDSES SSDSSSESGD SDSSSDSESE SSSEDEKKRK
AEPASEERPA KITKPSQDSN ETCTVFVGRL SWNVDDQWLG QEFEEYGTIV GARVIMDGQS
GRSKGYGYVD FETPEAAKAA VAANGTKEID GRMVNLDLSN PRPANPQPYA QQRAGNFGDQ
LSEPSDTVFV GNLSFNATED DLSTAFGGCG DIQSIRLPTD PQSGRLKGFG YVTFSDIDSA
KKCVEMNGHF IAGRPCRLDF STPRTGGGSR GGRGGFGGRG GFGGRGGFGG GRGRGRGGAR
SGNPNRGSVA PFSGNKVTFD
