ID   GARD_BACSU              Reviewed;         510 AA.
AC   P42240; P42241;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 3.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Probable galactarate dehydratase (L-threo-forming);
DE            Short=GalcD;
DE            EC=4.2.1.42 {ECO:0000250|UniProtKB:P39829};
GN   Name=garD; Synonyms=ycbH; OrderedLocusNames=BSU02510;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=7704254; DOI=10.1099/13500872-141-2-269;
RA   Ogawa K., Akagawa E., Nakamura K., Yamane K.;
RT   "Determination of a 21548 bp nucleotide sequence around the 24 degrees
RT   region of the Bacillus subtilis chromosome.";
RL   Microbiology 141:269-275(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 326.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- FUNCTION: Catalyzes the dehydration of galactarate to form 5-dehydro-4-
CC       deoxy-D-glucarate (5-KDG). {ECO:0000250|UniProtKB:P39829}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=galactarate = 5-dehydro-4-deoxy-D-glucarate + H2O;
CC         Xref=Rhea:RHEA:16005, ChEBI:CHEBI:15377, ChEBI:CHEBI:16537,
CC         ChEBI:CHEBI:42819; EC=4.2.1.42;
CC         Evidence={ECO:0000250|UniProtKB:P39829};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000250|UniProtKB:P39829};
CC   -!- PATHWAY: Carbohydrate acid metabolism; galactarate degradation; D-
CC       glycerate from galactarate: step 1/3. {ECO:0000250|UniProtKB:P39829}.
CC   -!- SIMILARITY: Belongs to the UxaA family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA06472.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAA06473.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; D30808; BAA06472.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; D30808; BAA06473.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AL009126; CAB12045.2; -; Genomic_DNA.
DR   PIR; C69753; C69753.
DR   RefSeq; NP_388133.2; NC_000964.3.
DR   RefSeq; WP_003246362.1; NZ_JNCM01000030.1.
DR   AlphaFoldDB; P42240; -.
DR   SMR; P42240; -.
DR   IntAct; P42240; 1.
DR   STRING; 224308.BSU02510; -.
DR   PaxDb; P42240; -.
DR   PRIDE; P42240; -.
DR   EnsemblBacteria; CAB12045; CAB12045; BSU_02510.
DR   GeneID; 938407; -.
DR   KEGG; bsu:BSU02510; -.
DR   PATRIC; fig|224308.179.peg.258; -.
DR   eggNOG; COG2721; Bacteria.
DR   InParanoid; P42240; -.
DR   OMA; DVGWELF; -.
DR   PhylomeDB; P42240; -.
DR   BioCyc; BSUB:BSU02510-MON; -.
DR   UniPathway; UPA00565; UER00629.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0008867; F:galactarate dehydratase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0019698; P:D-galacturonate catabolic process; IBA:GO_Central.
DR   GO; GO:0046392; P:galactarate catabolic process; IBA:GO_Central.
DR   CDD; cd11613; SAF_AH_GD; 1.
DR   InterPro; IPR007392; Gal/Altron_deHydtase_C.
DR   InterPro; IPR017654; GarD-like.
DR   InterPro; IPR013974; SAF.
DR   InterPro; IPR044144; UxaA/GarD_SAF.
DR   Pfam; PF04295; GD_AH_C; 1.
DR   Pfam; PF08666; SAF; 1.
DR   SMART; SM00858; SAF; 1.
DR   TIGRFAMs; TIGR03248; galactar-dH20; 1.
PE   3: Inferred from homology;
KW   Iron; Lyase; Metal-binding; Reference proteome.
FT   CHAIN           1..510
FT                   /note="Probable galactarate dehydratase (L-threo-forming)"
FT                   /id="PRO_0000172284"
FT   CONFLICT        326
FT                   /note="E -> K (in Ref. 1; BAA06473)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   510 AA;  54794 MW;  C1063C28575ACDED CRC64;
     MAMNLRKNQA PLYIKVHEID NTAIIVNDGG LPKGTVFSCG LVLEEDVPQG HKVALTDLNQ
     GDEIVRYGEV IGFADETIKR GSWIREALVR MPAPPALDDL PLANRVPQPR PPLEGYTFEG
     YRNADGSAGT KNILGITTSV QCVVGVLDYA VKRIKEELLP KYPNVDDVVP LHHQYGCGVA
     INAPDAVIPI RTIQNLAKHP NFGGEVMVIG LGCEKLLPER IASENDDDIL SLQDHRGFAA
     MIQSILEMAE ERLIRLNSRT RVSCPVSDLV IGLQCGGSDA FSGVTANPAV GYAADLLVRA
     GATVLFSEVT EVRDAIHLLT PRAVSEEVGQ SLIKEMKWYD SYLRRGDADR SANPSPGNKK
     GGLSNVVEKA LGSVAKSGTS PISGVLGPGE RAKQKGLLFA ATPASDFVCG TLQLAAGMNL
     QVFTTGRGTP YGLAAAPVLK VSTRHSLSEH WADLIDINAG RIATGEASIE DVGWEIFRTI
     LDVASGRKQT WADRWGLHND LCLFNPAPVT