GARE2_MOUSE
ID GARE2_MOUSE Reviewed; 880 AA.
AC Q6PAJ3; Q14CH4;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=GRB2-associated and regulator of MAPK protein 2;
DE AltName: Full=GRB2-associated and regulator of MAPK1-like;
GN Name=Garem2; Synonyms=Fam59b, Gareml, Gm444;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 41-880 (ISOFORM 1).
RC STRAIN=C57BL/6J;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable adapter protein that provides a critical link
CC between cell surface epidermal growth factor receptor and the MAPK/ERK
CC signaling pathway. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6PAJ3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6PAJ3-2; Sequence=VSP_034202, VSP_034203, VSP_034204;
CC -!- SIMILARITY: Belongs to the GAREM family. {ECO:0000305}.
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DR EMBL; AC112933; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC060268; AAH60268.1; -; mRNA.
DR EMBL; BC113782; AAI13783.1; -; mRNA.
DR EMBL; BC113783; AAI13784.1; -; mRNA.
DR CCDS; CCDS51449.1; -. [Q6PAJ3-1]
DR RefSeq; NP_001161351.1; NM_001167879.1. [Q6PAJ3-1]
DR AlphaFoldDB; Q6PAJ3; -.
DR SMR; Q6PAJ3; -.
DR BioGRID; 232473; 2.
DR STRING; 10090.ENSMUSP00000054208; -.
DR iPTMnet; Q6PAJ3; -.
DR PhosphoSitePlus; Q6PAJ3; -.
DR MaxQB; Q6PAJ3; -.
DR PaxDb; Q6PAJ3; -.
DR PRIDE; Q6PAJ3; -.
DR ProteomicsDB; 273031; -. [Q6PAJ3-1]
DR ProteomicsDB; 273032; -. [Q6PAJ3-2]
DR Antibodypedia; 57056; 71 antibodies from 19 providers.
DR Ensembl; ENSMUST00000058045; ENSMUSP00000054208; ENSMUSG00000044576. [Q6PAJ3-1]
DR GeneID; 242915; -.
DR KEGG; mmu:242915; -.
DR UCSC; uc012dtz.1; mouse. [Q6PAJ3-1]
DR CTD; 150946; -.
DR MGI; MGI:2685290; Garem2.
DR VEuPathDB; HostDB:ENSMUSG00000044576; -.
DR eggNOG; ENOG502QQU8; Eukaryota.
DR GeneTree; ENSGT00530000063834; -.
DR HOGENOM; CLU_014784_0_0_1; -.
DR InParanoid; Q6PAJ3; -.
DR OMA; PLFKPSH; -.
DR OrthoDB; 288834at2759; -.
DR PhylomeDB; Q6PAJ3; -.
DR TreeFam; TF329726; -.
DR BioGRID-ORCS; 242915; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q6PAJ3; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q6PAJ3; protein.
DR Bgee; ENSMUSG00000044576; Expressed in ventricular zone and 68 other tissues.
DR Genevisible; Q6PAJ3; MM.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR025946; CABIT_dom.
DR InterPro; IPR013761; SAM/pointed_sf.
DR Pfam; PF12736; CABIT; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..880
FT /note="GRB2-associated and regulator of MAPK protein 2"
FT /id="PRO_0000340254"
FT DOMAIN 813..877
FT /note="SAM"
FT REGION 12..320
FT /note="CABIT"
FT REGION 385..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 461..483
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 527..548
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 569..611
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 633..713
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 466..480
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 584..598
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 649..672
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 684..699
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 740
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q75VX8"
FT VAR_SEQ 1..119
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_034202"
FT VAR_SEQ 228..289
FT /note="FQCQGRFSTRSPLELQMQEGEHTVRAIIERVRLPVNVLVPSRPPRNPYDLHP
FT VREGHCYKLV -> LQCLLGVAAAAASSQAALRCCPTFPSCSLSTRPAPVSPIIPLACR
FT MGELLPWSLAFHFSFVF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_034203"
FT VAR_SEQ 290..880
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_034204"
SQ SEQUENCE 880 AA; 93510 MW; F672097E9DAEC8EB CRC64;
MEKLAAGLAG LRWSMGAFPL DLIVSRCRLP TLACLGPGEY AEGVSERDIL LIHSCRQWTT
VTAHTLEEGH YVIGPKIDIP LQYPGKFKLL EQARDVREPV RYFSSVEEVA SVFPDRIFVM
EAITFSVKVV SGEFSEDSEV YNFTLHAGDE LTLMGQAEIL CAKTTKERSR FTTLLRKLGR
AGALAGIGGP GSMGATGGGG GAARPVKSKM PCLICMNHRT NESLSLPFQC QGRFSTRSPL
ELQMQEGEHT VRAIIERVRL PVNVLVPSRP PRNPYDLHPV REGHCYKLVS IISKTVVLGL
ALRREGPAPL HFLLLTDTPR FTLPQGLLAG DPRVERLVRD SASYCRERFD PDEYSTAVRE
APAELSDDCA SPRHARLCLP APRAPGAVRA PGPPGRLGPA LPAPGDSDQD YVSPDWAGVS
EPAGGCAEIP YEELWVHQAP ESRADARARP LAGPDLISFG IVGPPRHEPE APPPPVPPKS
EAVKEECRLL HAPPVPPRGG GSCSKLTGSP PVLPHFPKLQ PVHSPSSSLS YYSSGLQDGA
GSRSGSGSPS PDAYSLYCYP CTWGDCKASE SSSRPPPGPL PSTTTQPSQA SRGLSEPLSG
RTPSLLGADT PVVKNYHSCP PLFKSSRPQK SFAPFGALNP FSGPAHPSGA PAASSSGSIS
TSGVLATSSP THSPGPGPGP QGQGYSAAPS SSLSSSEWQE PALEPLDPFE LGQASPPELE
LVRCQEPRAA GAPGSGPCLS PLGQPKAFEP EGLVLRQVPA SLSPAALQGP EAGGTLLFLT
QGCLEGPPGS PREGATGAGV RDASSWQPPA DLSALSLEEV SRSLRFIGLS EDVVSFFARE
RIDGSIFVQL SEDILADDFH LTKLQVKKIM QFIKGWRPKI
