GARL3_MOUSE
ID GARL3_MOUSE Reviewed; 1038 AA.
AC Q3V0G7; Q8BNH8; Q8CC22;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=GTPase-activating Rap/Ran-GAP domain-like protein 3;
GN Name=Garnl3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Diencephalon, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 188-197, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68; SER-449; SER-455 AND
RP THR-851, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6 (ISOFORM 2), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3V0G7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3V0G7-2; Sequence=VSP_029746, VSP_029747;
CC -!- SIMILARITY: Belongs to the GARNL3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC38990.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK034068; BAC28570.1; -; mRNA.
DR EMBL; AK083673; BAC38990.1; ALT_INIT; mRNA.
DR EMBL; AK133163; BAE21537.1; -; mRNA.
DR EMBL; AL731852; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC132143; AAI32144.1; -; mRNA.
DR CCDS; CCDS15938.1; -. [Q3V0G7-2]
DR CCDS; CCDS89476.1; -. [Q3V0G7-1]
DR RefSeq; NP_849219.2; NM_178888.4. [Q3V0G7-2]
DR RefSeq; XP_006498546.1; XM_006498483.3. [Q3V0G7-1]
DR RefSeq; XP_006498547.1; XM_006498484.3.
DR RefSeq; XP_006498548.1; XM_006498485.3. [Q3V0G7-1]
DR AlphaFoldDB; Q3V0G7; -.
DR SMR; Q3V0G7; -.
DR BioGRID; 221228; 8.
DR STRING; 10090.ENSMUSP00000099874; -.
DR iPTMnet; Q3V0G7; -.
DR PhosphoSitePlus; Q3V0G7; -.
DR MaxQB; Q3V0G7; -.
DR PaxDb; Q3V0G7; -.
DR PRIDE; Q3V0G7; -.
DR ProteomicsDB; 267558; -. [Q3V0G7-1]
DR ProteomicsDB; 267559; -. [Q3V0G7-2]
DR Antibodypedia; 16601; 49 antibodies from 10 providers.
DR DNASU; 99326; -.
DR Ensembl; ENSMUST00000102810; ENSMUSP00000099874; ENSMUSG00000038860. [Q3V0G7-2]
DR Ensembl; ENSMUST00000137381; ENSMUSP00000122576; ENSMUSG00000038860. [Q3V0G7-1]
DR GeneID; 99326; -.
DR KEGG; mmu:99326; -.
DR UCSC; uc008jhi.1; mouse. [Q3V0G7-2]
DR UCSC; uc008jhj.1; mouse. [Q3V0G7-1]
DR CTD; 84253; -.
DR MGI; MGI:2139309; Garnl3.
DR VEuPathDB; HostDB:ENSMUSG00000038860; -.
DR eggNOG; KOG3686; Eukaryota.
DR GeneTree; ENSGT00940000159362; -.
DR HOGENOM; CLU_008685_1_0_1; -.
DR InParanoid; Q3V0G7; -.
DR OMA; FEGYSER; -.
DR OrthoDB; 845465at2759; -.
DR PhylomeDB; Q3V0G7; -.
DR TreeFam; TF318626; -.
DR BioGRID-ORCS; 99326; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Garnl3; mouse.
DR PRO; PR:Q3V0G7; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q3V0G7; protein.
DR Bgee; ENSMUSG00000038860; Expressed in cerebellar cortex and 162 other tissues.
DR ExpressionAtlas; Q3V0G7; baseline and differential.
DR Genevisible; Q3V0G7; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
DR GO; GO:0090630; P:activation of GTPase activity; IBA:GO_Central.
DR GO; GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
DR Gene3D; 3.40.50.11210; -; 1.
DR InterPro; IPR001180; CNH_dom.
DR InterPro; IPR035974; Rap/Ran-GAP_sf.
DR InterPro; IPR000331; Rap/Ran_GAP_dom.
DR Pfam; PF00780; CNH; 1.
DR Pfam; PF02145; Rap_GAP; 1.
DR SUPFAM; SSF111347; SSF111347; 1.
DR PROSITE; PS50219; CNH; 1.
DR PROSITE; PS50085; RAPGAP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; GTPase activation;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..1038
FT /note="GTPase-activating Rap/Ran-GAP domain-like protein 3"
FT /id="PRO_0000312216"
FT DOMAIN 214..430
FT /note="Rap-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00165"
FT DOMAIN 512..824
FT /note="CNH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00795"
FT REGION 833..863
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 937..1038
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 833..853
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 937..968
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 971..985
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 68
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 449
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 455
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 851
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..45
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029746"
FT VAR_SEQ 46..48
FT /note="VNL -> MKS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029747"
FT CONFLICT 529
FT /note="T -> A (in Ref. 1; BAC38990)"
FT /evidence="ECO:0000305"
FT MOD_RES Q3V0G7-2:6
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 1038 AA; 115437 MW; 94E4B7A37AF5ADF6 CRC64;
MDPLTKGSCG SQLAQTLLWK AKSSLSFGIQ PLQTWPTKDP ELESQVNLSV SEDLGCRRGD
FSRKHYGSVE LLISSDADGA IQRAGRFRVE NGSTDESAAA LPGTWRRTDV HLENPEYHTR
WYFKYFLGQV HQNYIGNDAE KSPFFLSVTL SDQNNQRVPQ YRAILWRKTG TQKICLPYSP
TKTLSVKSIL SAMNLDKFEK GPREIFHPEI QKDLLVLEEQ EGSVNFKFGV LFAKDGQLTD
DEMFSNEIGS EAFQKFLNLL GDTITLKGWT GYRGGLDTKN NTTGINSVYT VYQGHEVMFH
VSTMLPYSKE NRQQVERKRH IGNDIVTIVF QEGEESSPAF KPSMIRSHFT HIFALVRYDQ
QNDNYRLKIF SEESVPLFGP PLPSPPVFTD HQEFRDFLLV KLINGEKATL ETPTFAQKRR
RTLDMLIRSL YQDLMPDLHK NMLNRRSFSD VLPESPKSAR KKEEARQAEF VRIGQALKLK
SIVRGDAPSS LAASGMCKKE PWEPQCFCCN FPHEAVCADP WGQALLVSTD AGVLLVDDDL
PSVPVFDRTL PVKQIHVLET LDLLVLRADK GKDARLFVFR LSAVQKGLDG RQTGRSRSDC
RENKLEKTKG CHLYAINTHH SRELRIVVAI RNKLLLITRK PHNKPSGVPG VSLLSPLSES
PVEEFQYIRE ICLCDSPAVM ALVDGPTEES DNLICVAYRH QFDVVNESTG EAFRLHHVEA
NKVNFVAAID VYEDGEAGLL LCYNYSCIYK KVCPFNGGSF LLQPSASDFQ FCWNQAPYAI
VCAFPYLLAF TTDSMEIRLV VNGNLVHTAV VPQLQLVASR SDIYFTAATT VHEGSSGGSS
KGASAHTSPQ TPPARDTPLF PSSLGEGEIQ SKNLYKIPLR NLVGRSIERP LKSPLVSKVI
TPPTSIGLGV AAIPVTHSLS LSRMEIKEIA SRTRRELLGL SDDGGTKTEG APRAKSKTRK
RLEESQGGPK PETVRSASSD RIPSGILESP ASEANPEGHN HWASSEQDAG VDKEGSPGSG
SSPFQLMASS EEDIIDLK
