ID   GAS1_MAGO7              Reviewed;         251 AA.
AC   G4MSQ1;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Appressoria-specific virulence factor GAS1 {ECO:0000303|PubMed:12215509};
DE   Flags: Precursor;
GN   Name=GAS1 {ECO:0000303|PubMed:12215509};
GN   Synonyms=MAS3 {ECO:0000250|UniProtKB:Q96TN6}; ORFNames=MGG_12337;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
RN   [2]
RP   INDUCTION, FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX   PubMed=12215509; DOI=10.1105/tpc.003426;
RA   Xue C., Park G., Choi W., Zheng L., Dean R.A., Xu J.R.;
RT   "Two novel fungal virulence genes specifically expressed in appressoria of
RT   the rice blast fungus.";
RL   Plant Cell 14:2107-2119(2002).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=23454094; DOI=10.1016/j.gep.2013.02.003;
RA   Jin Q., Li C., Li Y., Shang J., Li D., Chen B., Dong H.;
RT   "Complexity of roles and regulation of the PMK1-MAPK pathway in mycelium
RT   development, conidiation and appressorium formation in Magnaporthe
RT   oryzae.";
RL   Gene Expr. Patterns 13:133-141(2013).
CC   -!- FUNCTION: Appressoria-specific virulence factor required for
CC       appressorial penetration in host and lesion development.
CC       {ECO:0000269|PubMed:12215509, ECO:0000269|PubMed:23454094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12215509}.
CC       Note=Exclusively located in the cytoplasm of appressoria cells.
CC       {ECO:0000269|PubMed:12215509}.
CC   -!- INDUCTION: Expressed specifically during appressorium formation.
CC       {ECO:0000269|PubMed:12215509}.
CC   -!- DISRUPTION PHENOTYPE: Does not affect vegetative growth, conidiation,
CC       or appressoria formation, but reduces in appressorial penetration and
CC       lesion development. {ECO:0000269|PubMed:12215509,
CC       ECO:0000269|PubMed:23454094}.
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DR   EMBL; CM001232; EHA55472.1; -; Genomic_DNA.
DR   RefSeq; XP_003715279.1; XM_003715231.1.
DR   AlphaFoldDB; G4MSQ1; -.
DR   STRING; 318829.MGG_12337T0; -.
DR   EnsemblFungi; MGG_12337T0; MGG_12337T0; MGG_12337.
DR   GeneID; 5050935; -.
DR   KEGG; mgr:MGG_12337; -.
DR   VEuPathDB; FungiDB:MGG_12337; -.
DR   eggNOG; ENOG502QUBE; Eukaryota.
DR   HOGENOM; CLU_047729_3_2_1; -.
DR   InParanoid; G4MSQ1; -.
DR   OMA; MQCTGTV; -.
DR   OrthoDB; 1376474at2759; -.
DR   Proteomes; UP000009058; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR021476; Egh16-like.
DR   PANTHER; PTHR34618; PTHR34618; 1.
DR   Pfam; PF11327; Egh16-like; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Reference proteome; Signal; Virulence.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..251
FT                   /note="Appressoria-specific virulence factor GAS1"
FT                   /id="PRO_5003465464"
FT   REGION          40..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        57..76
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   251 AA;  26150 MW;  5239F0CB43F13CBF CRC64;
     MSLKSLIAAT ILAAPLVAGH GAIIKAVGDA GGEGMGLGVV TSTPRDGTRR DPFQQDSTRF
     KGQQADTFGE TVGGGQNDVE SMTKAIMAET GSQLPQISPG GSLQMTLHQV NGDGAGPYTC
     ELNDDASGAQ WQQIRVTTTP PGRNSRNRDG AMTDFPLVAE IPQRQACTGT VAGQTNVCLV
     RCMNAARAGP FGGVVAAQLA GSQTPQAARR ALAESVKRSE LIVSGLKKRG YNIADLSQEE
     IDELRRDGEI A