ID   GAS1_MAGOR              Reviewed;         251 AA.
AC   Q96TN6;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Appressoria-specific virulence factor GAS1 {ECO:0000303|PubMed:12215509};
DE   Flags: Precursor;
GN   Name=GAS1 {ECO:0000303|PubMed:12215509}; Synonyms=MAS3 {ECO:0000303|Ref.1};
OS   Magnaporthe oryzae (Rice blast fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=318829;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Choi W., Dean R.A.;
RT   "Sequence analysis and characterization of genes expressed during
RT   appressorium formation in rice blast fungus, Magnaporthe grisea.";
RL   Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, FUNCTION, DISRUPTION PHENOTYPE, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=Guyane 11;
RX   PubMed=12215509; DOI=10.1105/tpc.003426;
RA   Xue C., Park G., Choi W., Zheng L., Dean R.A., Xu J.R.;
RT   "Two novel fungal virulence genes specifically expressed in appressoria of
RT   the rice blast fungus.";
RL   Plant Cell 14:2107-2119(2002).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=23454094; DOI=10.1016/j.gep.2013.02.003;
RA   Jin Q., Li C., Li Y., Shang J., Li D., Chen B., Dong H.;
RT   "Complexity of roles and regulation of the PMK1-MAPK pathway in mycelium
RT   development, conidiation and appressorium formation in Magnaporthe
RT   oryzae.";
RL   Gene Expr. Patterns 13:133-141(2013).
CC   -!- FUNCTION: Appressoria-specific virulence factor required for
CC       appressorial penetration in host and lesion development.
CC       {ECO:0000269|PubMed:12215509, ECO:0000269|PubMed:23454094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12215509}.
CC       Note=Exclusively located in the cytoplasm of appressoria cells.
CC       {ECO:0000269|PubMed:12215509}.
CC   -!- INDUCTION: Expressed specifically during appressorium formation.
CC       {ECO:0000269|PubMed:12215509}.
CC   -!- DISRUPTION PHENOTYPE: Does not affect vegetative growth, conidiation,
CC       or appressoria formation, but redices in appressorial penetration and
CC       lesion development. {ECO:0000269|PubMed:12215509,
CC       ECO:0000269|PubMed:23454094}.
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DR   EMBL; AF363065; AAK52794.1; -; Genomic_DNA.
DR   EMBL; AF433133; AAL28112.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q96TN6; -.
DR   OMA; MQCTGTV; -.
DR   PHI-base; PHI:256; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR021476; Egh16-like.
DR   PANTHER; PTHR34618; PTHR34618; 1.
DR   Pfam; PF11327; Egh16-like; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Signal; Virulence.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..251
FT                   /note="Appressoria-specific virulence factor GAS1"
FT                   /id="PRO_5007715995"
FT   REGION          40..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        57..76
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   251 AA;  26150 MW;  5239F0CB43F13CBF CRC64;
     MSLKSLIAAT ILAAPLVAGH GAIIKAVGDA GGEGMGLGVV TSTPRDGTRR DPFQQDSTRF
     KGQQADTFGE TVGGGQNDVE SMTKAIMAET GSQLPQISPG GSLQMTLHQV NGDGAGPYTC
     ELNDDASGAQ WQQIRVTTTP PGRNSRNRDG AMTDFPLVAE IPQRQACTGT VAGQTNVCLV
     RCMNAARAGP FGGVVAAQLA GSQTPQAARR ALAESVKRSE LIVSGLKKRG YNIADLSQEE
     IDELRRDGEI A