GASA4_ARATH
ID GASA4_ARATH Reviewed; 106 AA.
AC P46690; O49593;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Gibberellin-regulated protein 4;
DE AltName: Full=GAST1 protein homolog 4;
DE Flags: Precursor;
GN Name=GASA4; OrderedLocusNames=At5g15230; ORFNames=F8M21.120;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. C24; TISSUE=Flower bud;
RX PubMed=7727751; DOI=10.1007/bf00020227;
RA Herzog M., Dorne A.-M., Grellet F.;
RT "GASA, a gibberellin-regulated gene family from Arabidopsis thaliana
RT related to the tomato GAST1 gene.";
RL Plant Mol. Biol. 27:743-752(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=9520278; DOI=10.1023/a:1005938624418;
RA Aubert D., Chevillard M., Dorne A.-M., Arlaud G., Herzog M.;
RT "Expression patterns of GASA genes in Arabidopsis thaliana: the GASA4 gene
RT is up-regulated by gibberellins in meristematic regions.";
RL Plant Mol. Biol. 36:871-883(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=17284469; DOI=10.1093/pcp/pcm016;
RA Roxrud I., Lid S.E., Fletcher J.C., Schmidt E.D., Opsahl-Sorteberg H.G.;
RT "GASA4, one of the 14-member Arabidopsis GASA family of small polypeptides,
RT regulates flowering and seed development.";
RL Plant Cell Physiol. 48:471-483(2007).
RN [7]
RP FUNCTION, AND MUTAGENESIS OF CYS-72; CYS-73; CYS-76 AND CYS-78.
RX PubMed=21143681; DOI=10.1111/j.1365-313x.2010.04390.x;
RA Rubinovich L., Weiss D.;
RT "The Arabidopsis cysteine-rich protein GASA4 promotes GA responses and
RT exhibits redox activity in bacteria and in planta.";
RL Plant J. 64:1018-1027(2010).
CC -!- FUNCTION: Gibberellin-regulated protein involved in the regulation of
CC floral meristem and floral organ identity, and promotion of seed size
CC and weight. May play a role in the promotion of gibberellin responses
CC such as regulation of flowering under short-day conditions, seed
CC germination and inhibition of gibberellin oxidase. Possesses redox
CC activity in E.coli and may function in redox regulation in planta.
CC {ECO:0000269|PubMed:17284469, ECO:0000269|PubMed:21143681}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=P46690-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in flower buds, style, stamen filaments,
CC vasculature of petals, root phloem, vasculature of cotyledons and
CC rosette leaves and developing embryo. {ECO:0000269|PubMed:17284469,
CC ECO:0000269|PubMed:9520278}.
CC -!- INDUCTION: By gibberellins. {ECO:0000269|PubMed:9520278}.
CC -!- PTM: Six disulfide bonds may be present.
CC -!- DISRUPTION PHENOTYPE: Increased number of axillary inflorescence shoots
CC and decreased seed weight. {ECO:0000269|PubMed:17284469}.
CC -!- SIMILARITY: Belongs to the GASA family. {ECO:0000305}.
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DR EMBL; U15683; AAA74480.1; -; mRNA.
DR EMBL; X98255; CAA66909.1; -; Genomic_DNA.
DR EMBL; AL353993; CAB89333.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92132.1; -; Genomic_DNA.
DR EMBL; AF360199; AAK25909.1; -; mRNA.
DR EMBL; AY057601; AAL14396.1; -; mRNA.
DR EMBL; AY040048; AAK64106.1; -; mRNA.
DR PIR; S60232; S60232.
DR PIR; T49958; T49958.
DR RefSeq; NP_197027.1; NM_121527.4. [P46690-1]
DR AlphaFoldDB; P46690; -.
DR SMR; P46690; -.
DR BioGRID; 16652; 5.
DR IntAct; P46690; 4.
DR STRING; 3702.AT5G15230.1; -.
DR PaxDb; P46690; -.
DR PRIDE; P46690; -.
DR ProteomicsDB; 247359; -. [P46690-1]
DR EnsemblPlants; AT5G15230.1; AT5G15230.1; AT5G15230. [P46690-1]
DR GeneID; 831375; -.
DR Gramene; AT5G15230.1; AT5G15230.1; AT5G15230. [P46690-1]
DR KEGG; ath:AT5G15230; -.
DR Araport; AT5G15230; -.
DR TAIR; locus:2150896; AT5G15230.
DR eggNOG; ENOG502S17T; Eukaryota.
DR HOGENOM; CLU_142643_1_0_1; -.
DR InParanoid; P46690; -.
DR OrthoDB; 1550655at2759; -.
DR PhylomeDB; P46690; -.
DR PRO; PR:P46690; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P46690; baseline and differential.
DR Genevisible; P46690; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0045454; P:cell redox homeostasis; IMP:TAIR.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; TAS:TAIR.
DR GO; GO:0009739; P:response to gibberellin; IMP:TAIR.
DR InterPro; IPR003854; GASA.
DR Pfam; PF02704; GASA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Gibberellin signaling pathway;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..106
FT /note="Gibberellin-regulated protein 4"
FT /id="PRO_0000021325"
FT MUTAGEN 72
FT /note="C->A: Loss of redox activity and ability to promote
FT gibberellin responses; when associated with A-73; A-76 and
FT A-78."
FT /evidence="ECO:0000269|PubMed:21143681"
FT MUTAGEN 73
FT /note="C->A: Loss of redox activity and ability to promote
FT gibberellin responses; when associated with A-72; A-76 and
FT A-78."
FT /evidence="ECO:0000269|PubMed:21143681"
FT MUTAGEN 76
FT /note="C->A: Loss of redox activity and ability to promote
FT gibberellin responses; when associated with A-72; A-73 and
FT A-78."
FT /evidence="ECO:0000269|PubMed:21143681"
FT MUTAGEN 78
FT /note="C->A: Loss of redox activity and ability to promote
FT gibberellin responses; when associated with A-72; A-73 and
FT A-76."
FT /evidence="ECO:0000269|PubMed:21143681"
FT CONFLICT 34
FT /note="S -> R (in Ref. 1; AAA74480)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 106 AA; 11996 MW; 63E7A1A964B07678 CRC64;
MAKSYGAIFL LTLIVLFMLQ TMVMASSGSN VKWSQKRYGP GSLKRTQCPS ECDRRCKKTQ
YHKACITFCN KCCRKCLCVP PGYYGNKQVC SCYNNWKTQE GGPKCP
