GASP2_MOUSE
ID GASP2_MOUSE Reviewed; 826 AA.
AC Q8BUY8; Q148X7;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=G-protein coupled receptor-associated sorting protein 2;
DE Short=GASP-2;
GN Name=Gprasp2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-352.
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275 AND SER-277, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=28096187; DOI=10.1136/jmedgenet-2016-104320;
RA Xing G., Yao J., Liu C., Wei Q., Qian X., Wu L., Lu Y., Cao X.;
RT "GPRASP2, a novel causative gene mutated in an X-linked recessive syndromic
RT hearing loss.";
RL J. Med. Genet. 54:426-430(2017).
CC -!- FUNCTION: May play a role in regulation of a variety of G-protein
CC coupled receptors. {ECO:0000250|UniProtKB:Q96D09}.
CC -!- SUBUNIT: Interacts with cytoplasmic tails of a variety of G-protein
CC coupled receptors such as muscarinic acetylcholine receptor M1/CHRM1
CC and calcitonin receptor/CALCR. {ECO:0000250|UniProtKB:Q96D09}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in the brain and the cochlea.
CC Also in lung and muscle tissues. Localized in multiple structures of
CC the cochlea, detected in the spiral ganglion, stria vascularis, spiral
CC ligament, inner and outer hair cells. {ECO:0000269|PubMed:28096187}.
CC -!- SIMILARITY: Belongs to the GPRASP family. {ECO:0000305}.
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DR EMBL; AL683822; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC117923; AAI17924.1; -; mRNA.
DR EMBL; BC117924; AAI17925.1; -; mRNA.
DR EMBL; AK081764; BAC38324.1; -; mRNA.
DR CCDS; CCDS53191.1; -.
DR RefSeq; NP_001156487.1; NM_001163015.1.
DR RefSeq; NP_001156488.1; NM_001163016.1.
DR RefSeq; NP_001156489.1; NM_001163017.1.
DR RefSeq; XP_017173986.1; XM_017318497.1.
DR AlphaFoldDB; Q8BUY8; -.
DR STRING; 10090.ENSMUSP00000134666; -.
DR iPTMnet; Q8BUY8; -.
DR PhosphoSitePlus; Q8BUY8; -.
DR MaxQB; Q8BUY8; -.
DR PaxDb; Q8BUY8; -.
DR PeptideAtlas; Q8BUY8; -.
DR PRIDE; Q8BUY8; -.
DR ProteomicsDB; 267421; -.
DR Antibodypedia; 351; 197 antibodies from 28 providers.
DR Ensembl; ENSMUST00000113136; ENSMUSP00000108761; ENSMUSG00000072966.
DR Ensembl; ENSMUST00000173804; ENSMUSP00000134666; ENSMUSG00000072966.
DR GeneID; 245607; -.
DR KEGG; mmu:245607; -.
DR UCSC; uc009uhs.2; mouse.
DR CTD; 114928; -.
DR MGI; MGI:2442071; Gprasp2.
DR VEuPathDB; HostDB:ENSMUSG00000072966; -.
DR eggNOG; ENOG502S6CE; Eukaryota.
DR GeneTree; ENSGT00940000162726; -.
DR HOGENOM; CLU_017437_0_0_1; -.
DR InParanoid; Q8BUY8; -.
DR OMA; WPREEAN; -.
DR OrthoDB; 860703at2759; -.
DR PhylomeDB; Q8BUY8; -.
DR TreeFam; TF335652; -.
DR BioGRID-ORCS; 245607; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Gprasp2; mouse.
DR PRO; PR:Q8BUY8; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q8BUY8; protein.
DR Bgee; ENSMUSG00000072966; Expressed in subparaventricular zone and 190 other tissues.
DR Genevisible; Q8BUY8; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0001540; F:amyloid-beta binding; IDA:MGI.
DR GO; GO:0001664; F:G protein-coupled receptor binding; ISO:MGI.
DR GO; GO:0061484; P:hematopoietic stem cell homeostasis; IMP:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR006911; ARM-rpt_dom.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR043374; BHLHb9/GASP-1/GASP-2.
DR PANTHER; PTHR46414; PTHR46414; 1.
DR Pfam; PF04826; Arm_2; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..826
FT /note="G-protein coupled receptor-associated sorting
FT protein 2"
FT /id="PRO_0000239054"
FT REGION 1..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 336..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 506..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..35
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 220..235
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..251
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 519..534
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 275
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 277
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 826 AA; 92802 MW; 01E28D8B629F36A6 CRC64;
MTGAEVETGS QAKPDKKPQE EVAGGAERES EAPLVVRPKV RPQAPATSGA RPKTETKSSS
RARPKTETQS VSGTRHRMSG ARPRSEAQLM SGARPKTDAR AVGGARPKTE AKPIPGARPK
GDAQAWAHSE FGAEAMPRAE RAHLSNSVTW PPVNVGSATV TKSKSLSMNT ELASMGSEIF
SGTQGQPGIE PWFGPREEAN MGSWCYPRPR AREETSNESA DENSTMSSFW TREETSIRSW
PREEVNTRSR HRAKHQTNAR SKPRSKQDPY IDSLSGSEDE ASNPFCFWAG ENTNDMFRAR
GRDEANARPK IRTKREDYFE DEDEIYKESW LLPGEEGNRF RRRDKEEPNK TLKNENEKDV
KNDETVEQES RLEEEVIIGS WFWAEQETNV EAGASAICDA EPGAEEGAIG GSLFWTEEKP
SLGAVARDEV RPESEEEALF GSWFWDRDEA CFDPNPTPVY TAKSRYRDPE EDLNLASRPK
TWDEVTIEFK PPCHGLGFPS PRPFIIPEGA SGNSEEKAKN AELGAEGEEQ DSVAQRDLPE
PEFPFQYDPS YRSVQEIREH LKARESAQPE NWSCTCIQCE LRISSAEFEE LLLLMDRIRD
PFIHEIAKIA MGMRTASQFT RDFIRDSGVV SLIEALMNYP SSRVRTNFLE NMVHMAPPYP
NLNMIETFIC QVCEETLSHS VNSPEQLTGM RMLRHLTITT DYHVLIANYV SGFLALLTTG
DARTKFHVLK MLLNLSDNPM VAKKLFSAKA LSIFVGLFNI EETNDNIQIV IKMFQNISNI
VKSGAMSLLD DDFSLEPLVS AFHEFEELAK QLQIQIDNQN DPEEGQ
