GASR_MOUSE
ID GASR_MOUSE Reviewed; 453 AA.
AC P56481;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Gastrin/cholecystokinin type B receptor;
DE Short=CCK-B receptor;
DE Short=CCK-BR;
DE AltName: Full=Cholecystokinin-2 receptor;
DE Short=CCK2-R;
GN Name=Cckbr;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RA Kopin A.S.;
RL Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ;
RX PubMed=10860839; DOI=10.1006/bbrc.2000.2875;
RA Lay J.M., Jenkins C., Friis-Hansen L., Samuelson L.C.;
RT "Structure and developmental expression of the mouse CCK-B receptor gene.";
RL Biochem. Biophys. Res. Commun. 272:837-842(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
CC -!- FUNCTION: Receptor for gastrin and cholecystokinin. The CCK-B receptors
CC occur throughout the central nervous system where they modulate
CC anxiety, analgesia, arousal, and neuroleptic activity. This receptor
CC mediates its action by association with G proteins that activate a
CC phosphatidylinositol-calcium second messenger system (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF019371; AAB71863.1; -; mRNA.
DR EMBL; AF264178; AAG09801.1; -; Genomic_DNA.
DR EMBL; AF264177; AAG09801.1; JOINED; Genomic_DNA.
DR EMBL; AK028325; BAC25881.1; -; mRNA.
DR CCDS; CCDS21651.1; -.
DR RefSeq; NP_031653.1; NM_007627.5.
DR AlphaFoldDB; P56481; -.
DR SMR; P56481; -.
DR STRING; 10090.ENSMUSP00000033189; -.
DR BindingDB; P56481; -.
DR ChEMBL; CHEMBL2854; -.
DR DrugCentral; P56481; -.
DR GuidetoPHARMACOLOGY; 77; -.
DR GlyGen; P56481; 3 sites.
DR iPTMnet; P56481; -.
DR PhosphoSitePlus; P56481; -.
DR PaxDb; P56481; -.
DR PRIDE; P56481; -.
DR ProteomicsDB; 267422; -.
DR Antibodypedia; 11193; 363 antibodies from 37 providers.
DR DNASU; 12426; -.
DR Ensembl; ENSMUST00000033189; ENSMUSP00000033189; ENSMUSG00000030898.
DR GeneID; 12426; -.
DR KEGG; mmu:12426; -.
DR UCSC; uc009iyd.1; mouse.
DR CTD; 887; -.
DR MGI; MGI:99479; Cckbr.
DR VEuPathDB; HostDB:ENSMUSG00000030898; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244933; -.
DR HOGENOM; CLU_009579_6_3_1; -.
DR InParanoid; P56481; -.
DR OMA; NTTAHKC; -.
DR OrthoDB; 1042780at2759; -.
DR PhylomeDB; P56481; -.
DR TreeFam; TF315303; -.
DR Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR Reactome; R-MMU-881907; Gastrin-CREB signalling pathway via PKC and MAPK.
DR BioGRID-ORCS; 12426; 1 hit in 72 CRISPR screens.
DR PRO; PR:P56481; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P56481; protein.
DR Bgee; ENSMUSG00000030898; Expressed in retrosplenial region and 55 other tissues.
DR ExpressionAtlas; P56481; baseline and differential.
DR Genevisible; P56481; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0004951; F:cholecystokinin receptor activity; ISO:MGI.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0015054; F:gastrin receptor activity; ISS:UniProtKB.
DR GO; GO:0017046; F:peptide hormone binding; ISO:MGI.
DR GO; GO:0031741; F:type B gastrin/cholecystokinin receptor binding; ISO:MGI.
DR GO; GO:0006915; P:apoptotic process; ISO:MGI.
DR GO; GO:0002209; P:behavioral defense response; ISO:MGI.
DR GO; GO:0038188; P:cholecystokinin signaling pathway; ISO:MGI.
DR GO; GO:0048565; P:digestive tract development; IMP:MGI.
DR GO; GO:0070371; P:ERK1 and ERK2 cascade; ISO:MGI.
DR GO; GO:0044849; P:estrous cycle; ISO:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0001696; P:gastric acid secretion; IMP:MGI.
DR GO; GO:0048732; P:gland development; IMP:MGI.
DR GO; GO:0001821; P:histamine secretion; ISO:MGI.
DR GO; GO:0045851; P:pH reduction; IMP:MGI.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:2000987; P:positive regulation of behavioral fear response; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISS:UniProtKB.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISS:UniProtKB.
DR GO; GO:0090274; P:positive regulation of somatostatin secretion; ISO:MGI.
DR GO; GO:0050806; P:positive regulation of synaptic transmission; ISO:MGI.
DR GO; GO:0032230; P:positive regulation of synaptic transmission, GABAergic; ISO:MGI.
DR GO; GO:0051968; P:positive regulation of synaptic transmission, glutamatergic; ISO:MGI.
DR GO; GO:0051930; P:regulation of sensory perception of pain; ISO:MGI.
DR InterPro; IPR009126; Cholcskin_rcpt.
DR InterPro; IPR000314; Gastrin_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01822; CCYSTOKININR.
DR PRINTS; PR00527; GASTRINR.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Lipoprotein; Membrane; Palmitate; Receptor; Reference proteome; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..453
FT /note="Gastrin/cholecystokinin type B receptor"
FT /id="PRO_0000069475"
FT TOPO_DOM 1..57
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..79
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..87
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..109
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..131
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..150
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..170
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..189
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..219
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..242
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..361
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362..379
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 401..453
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 257..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 414
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:P17124"
FT CARBOHYD 7
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 30
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 127..205
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 453 AA; 49172 MW; DFD21432C323405C CRC64;
MDLLKLNRSL QGPGPGSGSS LCRPGVSLLN SSSAGNLSCE TPRIRGTGTR ELELTIRITL
YAVIFLMSVG GNVLIIVVLG LSRRLRTVTN AFLLSLAVSD LLLAVACMPF TLLPNLMGTF
IFGTVICKAV SYLMGVSVSV STLNLAAIAL ERYSAICRPL QARVWQTRSH AARVILATWL
LSGLLMVPYP VYTVVQPVGP RILQCMHLWP SERVQQMWSV LLLILLFFIP GVVMAVAYGL
ISRELYLGLR FDGDNDSETQ SRVRNQGGLP GGAAAPGPVH QNGGCRHVTS LTGEDSDGCY
VQLPRSRLEM TTLTTPTTGP GPGPRPNQAK LLAKKRVVRM LLVIVLLFFV CWLPVYSANT
WRAFDGPGAR RALAGAPISF IHLLSYTSAC ANPLVYCFMH RRFRQACLDT CARCCPRPPR
ARPRPLPDED PPTPSIASLS RLSYTTISTL GPG
