GAT18_ARATH
ID GAT18_ARATH Reviewed; 295 AA.
AC Q8LC79; C0SVE1; Q9SVL2;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=GATA transcription factor 18 {ECO:0000303|PubMed:15084732};
DE AltName: Full=Protein HANABA TARANU {ECO:0000303|PubMed:15367721};
GN Name=GATA18 {ECO:0000303|PubMed:15084732};
GN Synonyms=HAN {ECO:0000303|PubMed:15367721};
GN OrderedLocusNames=At3g50870 {ECO:0000312|Araport:AT3G50870};
GN ORFNames=F18B3.150 {ECO:0000312|EMBL:CAB42916.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Fujita M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-180, TISSUE SPECIFICITY,
RP GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia, cv. Landsberg erecta, and cv. Wassilewskija;
RX PubMed=15367721; DOI=10.1105/tpc.104.024869;
RA Zhao Y., Medrano L., Ohashi K., Fletcher J.C., Yu H., Sakai H.,
RA Meyerowitz E.M.;
RT "HANABA TARANU is a GATA transcription factor that regulates shoot apical
RT meristem and flower development in Arabidopsis.";
RL Plant Cell 16:2586-2600(2004).
RN [6]
RP GENE FAMILY ORGANIZATION.
RX PubMed=15084732; DOI=10.1104/pp.103.037788;
RA Reyes J.C., Muro-Pastor M.I., Florencio F.J.;
RT "The GATA family of transcription factors in Arabidopsis and rice.";
RL Plant Physiol. 134:1718-1732(2004).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-180, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=20643354; DOI=10.1016/j.devcel.2010.06.004;
RA Nawy T., Bayer M., Mravec J., Friml J., Birnbaum K.D., Lukowitz W.;
RT "The GATA factor HANABA TARANU is required to position the proembryo
RT boundary in the early Arabidopsis embryo.";
RL Dev. Cell 19:103-113(2010).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=22669825; DOI=10.1242/dev.081547;
RA Kanei M., Horiguchi G., Tsukaya H.;
RT "Stable establishment of cotyledon identity during embryogenesis in
RT Arabidopsis by ANGUSTIFOLIA3 and HANABA TARANU.";
RL Development 139:2436-2446(2012).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, REPRESSION BY HAN, SUBUNIT, AND INTERACTION
RP WITH GATA22; GATA21 AND GATA19.
RX PubMed=23335616; DOI=10.1105/tpc.112.107854;
RA Zhang X., Zhou Y., Ding L., Wu Z., Liu R., Meyerowitz E.M.;
RT "Transcription repressor HANABA TARANU controls flower development by
RT integrating the actions of multiple hormones, floral organ specification
RT genes, and GATA3 family genes in Arabidopsis.";
RL Plant Cell 25:83-101(2013).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH JAG AND AGO10/PNH, AND
RP TISSUE SPECIFICITY.
RX PubMed=26390296; DOI=10.1371/journal.pgen.1005479;
RA Ding L., Yan S., Jiang L., Zhao W., Ning K., Zhao J., Liu X., Zhang J.,
RA Wang Q., Zhang X.;
RT "HANABA TARANU (HAN) bridges meristem and organ primordia boundaries
RT through PINHEAD, JAGGED, BLADE-ON-PETIOLE2 and CYTOKININ OXIDASE 3 during
RT flower development in Arabidopsis.";
RL PLoS Genet. 11:E1005479-E1005479(2015).
CC -!- FUNCTION: Transcriptional factor that specifically binds 5'-GATA-3' or
CC 5'-GAT-3' motifs within gene promoters (including its own promoter and
CC GATA21 promoter), thus regulating the expression of genes mostly
CC involved in hormone responses and floral organ specification (including
CC genes regulating hormones responses) (PubMed:23335616,
CC PubMed:26390296). Regulates both flower and shoot apical meristem (SAM)
CC development, especially for establishing organ boundaries in shoots and
CC flowers, probably by controlling the number and position of WUS-
CC expressing cells (PubMed:15367721, PubMed:23335616). Coregulates, with
CC AGO10/PNH, the shoot apical meristem (SAM) organization. Regulates
CC floral organ development via the promotion of JAG and NPR5/BOP2
CC expression. Modulates cytokinin homeostasis in organ boundaries by
CC regulating CKX3 expression (PubMed:26390296). Involved in cell
CC proliferation and differentiation (PubMed:15367721). Required to
CC position the inductive proembryo boundary via the regulation of gene
CC expression and for early embryonic development (PubMed:20643354).
CC Together with GIF1/AN3, mediates cotyledon identity by preventing
CC ectopic root formation through the repression of PLT1 expression
CC (PubMed:22669825). {ECO:0000269|PubMed:15367721,
CC ECO:0000269|PubMed:20643354, ECO:0000269|PubMed:22669825,
CC ECO:0000269|PubMed:23335616, ECO:0000269|PubMed:26390296}.
CC -!- SUBUNIT: Homodimer (PubMed:23335616, PubMed:26390296). Forms
CC heterodimers with GATA19, GATA22 and GATA21 (PubMed:23335616).
CC Interacts with JAG. Binds to AGO10/PNH (PubMed:26390296).
CC {ECO:0000269|PubMed:23335616, ECO:0000269|PubMed:26390296}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20643354}.
CC -!- TISSUE SPECIFICITY: Expressed in vegetative and inflorescence shoot
CC apical meristems (SAMs), axillary (SAMs), floral meristems, developing
CC ovules and stamens, vascular tissues, and in the embryo.
CC {ECO:0000269|PubMed:15367721, ECO:0000269|PubMed:26390296}.
CC -!- DEVELOPMENTAL STAGE: In the developing axillary shoot apical meristem
CC (SAM), expressed at the boundary between nascent axillary meristems and
CC the adaxial side of leaves. In all mature SAMs, located at the
CC boundaries between the central SAM and the initiating organ primordia,
CC as well as between the neighboring initiating organ primordia. In the
CC floral meristem, strongly expressed at the boundaries between the
CC meristematic dome and the initiating floral organ primordia, and also
CC at the boundaries between the primordia of different whorls. Expression
CC at the boundaries attenuates as the organ primordia grow apart. In
CC flowers, localized at the boundary between the central meristematic
CC cells and differentiating stamen primordia to later accumulates at the
CC medial ridge region of the carpel. Highly expressed in the developing
CC anthers, in both the tapetum cell layer and microsporocytes. In
CC developing ovules, confined to inner and outer integuments. In aerial
CC tissues, strongly present in phloem tissues (PubMed:15367721). First
CC observed in the whole embryo, but later confined to the center cells of
CC the embryo and provascular tissues (PubMed:15367721, PubMed:20643354).
CC {ECO:0000269|PubMed:15367721, ECO:0000269|PubMed:20643354}.
CC -!- INDUCTION: Repressed via a negative regulatory feedback loop.
CC {ECO:0000269|PubMed:23335616}.
CC -!- DISRUPTION PHENOTYPE: Abnormal flower and shoot apical meristem (SAM)
CC development with fused sepals and reduced organ numbers in all four
CC whorls as well as flatter and smaller meristems (PubMed:15367721,
CC PubMed:23335616). Reduced levels of gibberellins (GA) but increased
CC content of trans-zeatin riboside (ZR) and auxin (IAA)
CC (PubMed:26390296). Coordinated apical shift of gene expression patterns
CC in the basal proembryo, including auxin transporter genes and resulting
CC in apical redistribution of auxin (PubMed:20643354). Altered WUS
CC expression from early embryogenesis (e.g. globular stage)
CC (PubMed:15367721). The double mutant an3 han-30 exhibits severe defects
CC in cotyledon development such as ectopic roots formation at the apical
CC region of the embryo and associated with an abnormal expansion of PLT1
CC expression from the basal embryonic region to the apical region
CC (PubMed:22669825). The double mutant pnh-2 han-2 has smaller
CC inflorescence meristems (IM) and taller floral meristems (FM) leading
CC to fewer petals. The double mutant han-2 jag-3 exhibits reduced petal
CC numbers, more serrated sepals and narrower petals (PubMed:26390296).
CC {ECO:0000269|PubMed:15367721, ECO:0000269|PubMed:20643354,
CC ECO:0000269|PubMed:22669825, ECO:0000269|PubMed:23335616,
CC ECO:0000269|PubMed:26390296}.
CC -!- MISCELLANEOUS: 'Hanaba taranu' means 'fewer floral leaves' in Japanese.
CC {ECO:0000303|PubMed:15367721}.
CC -!- SIMILARITY: Belongs to the type IV zinc-finger family. Class B
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM63797.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB42916.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB493644; BAH30482.1; -; mRNA.
DR EMBL; AL049862; CAB42916.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002686; AEE78720.1; -; Genomic_DNA.
DR EMBL; AY086746; AAM63797.1; ALT_INIT; mRNA.
DR PIR; T08408; T08408.
DR RefSeq; NP_566939.1; NM_114947.3.
DR AlphaFoldDB; Q8LC79; -.
DR SMR; Q8LC79; -.
DR BioGRID; 9569; 11.
DR IntAct; Q8LC79; 7.
DR STRING; 3702.AT3G50870.1; -.
DR PaxDb; Q8LC79; -.
DR PRIDE; Q8LC79; -.
DR ProteomicsDB; 248607; -.
DR EnsemblPlants; AT3G50870.1; AT3G50870.1; AT3G50870.
DR GeneID; 824251; -.
DR Gramene; AT3G50870.1; AT3G50870.1; AT3G50870.
DR KEGG; ath:AT3G50870; -.
DR Araport; AT3G50870; -.
DR TAIR; locus:2077932; AT3G50870.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_062129_1_0_1; -.
DR InParanoid; Q8LC79; -.
DR OMA; VFSMQNG; -.
DR OrthoDB; 1472671at2759; -.
DR PhylomeDB; Q8LC79; -.
DR PRO; PR:Q8LC79; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LC79; baseline and differential.
DR Genevisible; Q8LC79; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0048825; P:cotyledon development; IMP:UniProtKB.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009908; P:flower development; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0009909; P:regulation of flower development; IMP:UniProtKB.
DR GO; GO:0009934; P:regulation of meristem structural organization; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEP:TAIR.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR044272; GATA18/19/20.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR46813; PTHR46813; 1.
DR Pfam; PF00320; GATA; 1.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS00344; GATA_ZN_FINGER_1; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..295
FT /note="GATA transcription factor 18"
FT /id="PRO_0000083448"
FT ZN_FING 148..202
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT MUTAGEN 180
FT /note="G->D: In han-21; reduced fertility. Coordinated
FT apical shift of gene expression patterns in the basal
FT proembryo."
FT /evidence="ECO:0000269|PubMed:20643354"
FT MUTAGEN 180
FT /note="G->S: In han-2; reduced fertility. Abnormal flower
FT and shoot apical meristem (SAM) development with fused
FT sepals and reduced organ numbers in all four whorls as well
FT as flatter and smaller meristems. Coordinated apical shift
FT of gene expression patterns in the basal proembryo."
FT /evidence="ECO:0000269|PubMed:15367721,
FT ECO:0000269|PubMed:20643354"
FT CONFLICT 138
FT /note="G -> D (in Ref. 4; AAM63797)"
FT /evidence="ECO:0000305"
FT CONFLICT 194
FT /note="T -> S (in Ref. 4; AAM63797)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 295 AA; 31832 MW; 9127F4D8BD09DA60 CRC64;
MMQTPYTTST QGQYCHSCGM FHHHSQSCCY NNNNNSNAGS YSMVFSMQNG GVFEQNGEDY
HHSSSLVDCT LSLGTPSTRL CEEDEKRRRS TSSGASSCIS NFWDLIHTKN NNSKTAPYNN
VPSFSANKPS RGCSGGGGGG GGGGGGDSLL ARRCANCDTT STPLWRNGPR GPKSLCNACG
IRFKKEERRT TAATGNTVVG AAPVQTDQYG HHNSGYNNYH AATNNNNNNG TPWAHHHSTQ
RVPCNYPANE IRFMDDYGSG VANNVESDGA HGGVPFLSWR LNVADRASLV HDFTR
