GAT1_ARATH
ID GAT1_ARATH Reviewed; 451 AA.
AC F4HW02; Q9SGD7;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=GABA transporter 1;
DE Short=AtGAT1;
DE AltName: Full=Bidirectional amino acid transporter 1;
GN Name=GAT1; Synonyms=BAT1; OrderedLocusNames=At1g08230; ORFNames=T23G18.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17229318; DOI=10.1186/1471-2164-8-18;
RA Moskal W.A. Jr., Wu H.C., Underwood B.A., Wang W., Town C.D., Xiao Y.-L.;
RT "Experimental validation of novel genes predicted in the un-annotated
RT regions of the Arabidopsis genome.";
RL BMC Genomics 8:18-18(2007).
RN [4]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=16407306; DOI=10.1074/jbc.m510766200;
RA Meyer A., Eskandari S., Grallath S., Rentsch D.;
RT "AtGAT1, a high affinity transporter for gamma-aminobutyric acid in
RT Arabidopsis thaliana.";
RL J. Biol. Chem. 281:7197-7204(2006).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19199104; DOI=10.1007/s00425-009-0892-8;
RA Duendar E., Bush D.R.;
RT "BAT1, a bidirectional amino acid transporter in Arabidopsis.";
RL Planta 229:1047-1056(2009).
CC -!- FUNCTION: High affinity gamma-aminobutyric acid (GABA) transporter
CC probably involved in GABA uptake into cells. When expressed in a
CC heterologous system (Xenopus oocytes), imports GABA, butylamine,
CC beta- and L-Alanine, 5-aminovaleric acid, 6-aminocaproic acid and 8-
CC aminocaprylic acid, but does not mediate the transport of proline or
CC glycine betaine. {ECO:0000269|PubMed:16407306,
CC ECO:0000269|PubMed:19199104}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=10 uM for GABA {ECO:0000269|PubMed:16407306};
CC pH dependence:
CC Optimum pH is 4.5-5.5. {ECO:0000269|PubMed:16407306};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:16407306};
CC Multi-pass membrane protein {ECO:0000305|PubMed:16407306}. Note=Plasma
CC membrane.
CC -!- TISSUE SPECIFICITY: Highly expressed in flowers and at lower levels in
CC roots, leaves and stems. {ECO:0000269|PubMed:16407306,
CC ECO:0000269|PubMed:19199104}.
CC -!- INDUCTION: By wounding and senescence. {ECO:0000269|PubMed:16407306}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF18251.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=EF183286; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC011438; AAF18251.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28264.1; -; Genomic_DNA.
DR EMBL; EF183286; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001077487.1; NM_001084018.3.
DR AlphaFoldDB; F4HW02; -.
DR SMR; F4HW02; -.
DR BioGRID; 22585; 7.
DR IntAct; F4HW02; 6.
DR STRING; 3702.AT1G08230.2; -.
DR PaxDb; F4HW02; -.
DR PRIDE; F4HW02; -.
DR ProteomicsDB; 228899; -.
DR EnsemblPlants; AT1G08230.2; AT1G08230.2; AT1G08230.
DR GeneID; 837344; -.
DR Gramene; AT1G08230.2; AT1G08230.2; AT1G08230.
DR KEGG; ath:AT1G08230; -.
DR Araport; AT1G08230; -.
DR TAIR; locus:2199978; AT1G08230.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_031160_1_0_1; -.
DR InParanoid; F4HW02; -.
DR OMA; CGYHLST; -.
DR OrthoDB; 570025at2759; -.
DR PRO; PR:F4HW02; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HW02; baseline and differential.
DR Genevisible; F4HW02; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015185; F:gamma-aminobutyric acid transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0015812; P:gamma-aminobutyric acid transport; IGI:TAIR.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..451
FT /note="GABA transporter 1"
FT /id="PRO_0000418998"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 451 AA; 49695 MW; 1DBC49E86E3088C5 CRC64;
MGGEERSGDG EKRGEEVVDA GSLFVLKSKG TWWHCGFHLT TSIVAPALLS LPYAFKFLGW
AAGISCLVGG AAVTFYSYTL LSLTLEHHAS LGNRYLRFRD MAHHILSPKW GRYYVGPIQM
AVCYGVVIAN ALLGGQCLKA MYLVVQPNGE MKLFEFVIIF GCLLLVLAQF PSFHSLRYIN
SLSLLLCLLY SASAAAASIY IGKEPNAPEK DYTIVGDPET RVFGIFNAMA IIATTYGNGI
IPEIQATISA PVKGKMMKGL CMCYLVVIMT FFTVAITGYW AFGKKANGLI FTNFLNAETN
HYFVPTWFIF LVNLFTVLQL SAVAVVYLQP INDILESVIS DPTKKEFSIR NVIPRLVVRS
LFVVMATIVA AMLPFFGDVN SLLGAFGFIP LDFVLPVVFF NFTFKPSKKS FIFWINTVIA
VVFSCLGVIA MVAAVRQIII DANTYKLFAD V
