GAT1_CANAL
ID GAT1_CANAL Reviewed; 688 AA.
AC Q5A432; A0A1D8PMF2;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Transcriptional regulatory protein GAT1;
GN Name=GAT1; OrderedLocusNames=CAALFM_C405880WA;
GN ORFNames=CaO19.1275, CaO19.8862;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION.
RX PubMed=14617156; DOI=10.1046/j.1365-2958.2003.03747.x;
RA Limjindaporn T., Khalaf R.A., Fonzi W.A.;
RT "Nitrogen metabolism and virulence of Candida albicans require the GATA-
RT type transcriptional activator encoded by GAT1.";
RL Mol. Microbiol. 50:993-1004(2003).
RN [5]
RP FUNCTION.
RX PubMed=17369441; DOI=10.1128/ec.00307-06;
RA Dabas N., Morschhauser J.;
RT "Control of ammonium permease expression and filamentous growth by the GATA
RT transcription factors GLN3 and GAT1 in Candida albicans.";
RL Eukaryot. Cell 6:875-888(2007).
RN [6]
RP FUNCTION.
RX PubMed=17950010; DOI=10.1016/j.fgb.2007.08.006;
RA Liao W.L., Ramon A.M., Fonzi W.A.;
RT "GLN3 encodes a global regulator of nitrogen metabolism and virulence of C.
RT albicans.";
RL Fungal Genet. Biol. 45:514-526(2008).
RN [7]
RP FUNCTION.
RX PubMed=18547391; DOI=10.1111/j.1365-2958.2008.06297.x;
RA Dabas N., Morschhauser J.;
RT "A transcription factor regulatory cascade controls secreted aspartic
RT protease expression in Candida albicans.";
RL Mol. Microbiol. 69:586-602(2008).
RN [8]
RP INDUCTION.
RX PubMed=19197361; DOI=10.1371/journal.ppat.1000294;
RA Bastidas R.J., Heitman J., Cardenas M.E.;
RT "The protein kinase Tor1 regulates adhesin gene expression in Candida
RT albicans.";
RL PLoS Pathog. 5:E1000294-E1000294(2009).
RN [9]
RP FUNCTION.
RX PubMed=20884691; DOI=10.1099/mic.0.045005-0;
RA Navarathna D.H., Das A., Morschhauser J., Nickerson K.W., Roberts D.D.;
RT "Dur3 is the major urea transporter in Candida albicans and is co-regulated
RT with the urea amidolyase Dur1,2.";
RL Microbiology 157:270-279(2011).
RN [10]
RP FUNCTION.
RX PubMed=22194462; DOI=10.1128/ec.05200-11;
RA Chen Y.T., Lin C.Y., Tsai P.W., Yang C.Y., Hsieh W.P., Lan C.Y.;
RT "Rhb1 regulates the expression of secreted aspartic protease 2 through the
RT TOR signaling pathway in Candida albicans.";
RL Eukaryot. Cell 11:168-182(2012).
CC -!- FUNCTION: Transcriptional regulator of nitrogen utilization required
CC for nitrogen catabolite repression and utilization of isoleucine,
CC tyrosine and tryptophan as nitrogen sources. Controls expression of the
CC MEP2 ammonium permease, the DUR1,2 urea amidolyase, and the
CC transcription factor STP1, which in turn mediates SAP2 expression, a
CC long-known virulence attribute of C.albicans. Influences the
CC filamentation process depending upon the nitrogen sources available.
CC Required for virulence in a mouse systemic infection model.
CC {ECO:0000269|PubMed:14617156, ECO:0000269|PubMed:17369441,
CC ECO:0000269|PubMed:17950010, ECO:0000269|PubMed:18547391,
CC ECO:0000269|PubMed:20884691, ECO:0000269|PubMed:22194462}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Expression is induced by rapamycine.
CC {ECO:0000269|PubMed:19197361}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP017626; AOW29318.1; -; Genomic_DNA.
DR RefSeq; XP_716583.1; XM_711490.1.
DR AlphaFoldDB; Q5A432; -.
DR SMR; Q5A432; -.
DR STRING; 237561.Q5A432; -.
DR PRIDE; Q5A432; -.
DR GeneID; 3641794; -.
DR KEGG; cal:CAALFM_C405880WA; -.
DR CGD; CAL0000197994; GAT1.
DR VEuPathDB; FungiDB:C4_05880W_A; -.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_021528_0_0_1; -.
DR InParanoid; Q5A432; -.
DR OrthoDB; 1466754at2759; -.
DR PRO; PR:Q5A432; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:CGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:CGD.
DR GO; GO:0006808; P:regulation of nitrogen utilization; IMP:CGD.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR013860; AreA_GATA.
DR InterPro; IPR039355; Transcription_factor_GATA.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR10071; PTHR10071; 1.
DR Pfam; PF08550; DUF1752; 1.
DR Pfam; PF00320; GATA; 1.
DR PRINTS; PR00619; GATAZNFINGER.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS00344; GATA_ZN_FINGER_1; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 2: Evidence at transcript level;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Virulence; Zinc; Zinc-finger.
FT CHAIN 1..688
FT /note="Transcriptional regulatory protein GAT1"
FT /id="PRO_0000422807"
FT ZN_FING 438..462
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 83..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 259..278
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 343..438
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 482..565
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 639..678
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..116
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 343..357
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..384
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 395..438
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 486..511
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 522..565
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 688 AA; 75488 MW; C8B9197110E2F9FC CRC64;
MYYRARHSLP YQKRMENLTW RMMYINNKSI FTNTNNAPKE IFEQSLDPEI DDFDYVAHIK
KIGQFNQQKT QQQQDHLQSN LDNHNSIFAD NTGFSNNNDR GSSGGGMTSI SSLSKKRPAP
FSPMIQPEKT TIIPTATNTM SQLSQQLNEF NKFNHPSQTS NFNDVNHHME ISTSHIAPTS
SAFEFSLDPL AFEGPNQNFQ PEPHHDFNTN SFDSMTSSYE RPLFDDFLPR DHHNIQSSSV
PTSASSFSTI VPKNTQFSTS ASITSPTSTF SNQGNNSSNF HRLNSTVSIT ATPGNLLRQE
SMVSLPDYAN HLRSMSQTPT MNSSNAPFSH SFNDGGSYFM NNFTGITLPS QPSPQPIHFD
NKPKDDHFNT SLSVSQQQPS AKKSKRKSTI TKSKKKAASP ETTITSTGST ITTKSTNSNS
TGKGTATGPA ASNTGVSCTN CGTKTTPLWR RNPQGQPLCN ACGLFLKLHG VVRPLSLKTD
VIKKRQRGNN NGSGNSSGTT NNSNNYNNKS ISKKNEIDDG DDLNPTSITN NTGLTNNNNS
KSPAKSKKKS NFDNNSNSAL NNLDKSKLKI NTNEITNISE TTSNSSSPVI NLNHGGRSSG
VFGNTPDYLN GITSPAVSLV KSEIDNPHQL NNSNSNGMLM TMHQSSHQSS LSTTFDHEVE
SNNEGSNSSG VNTSTANNQD WDWLNMNY
