GAT1_YEAST
ID GAT1_YEAST Reviewed; 510 AA.
AC P43574; D6VTK9;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Transcriptional regulatory protein GAT1;
GN Name=GAT1; OrderedLocusNames=YFL021W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / GRF88;
RX PubMed=8622686; DOI=10.1128/mcb.16.3.847;
RA Coffman J.A., Rai R., Cunningham T., Svetlov V., Cooper T.G.;
RT "Gat1p, a GATA family protein whose production is sensitive to nitrogen
RT catabolite repression, participates in transcriptional activation of
RT nitrogen-catabolic genes in Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 16:847-858(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270; SER-399 AND SER-418, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167; SER-262 AND SER-418, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262; SER-270; THR-369 AND
RP SER-399, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Positive regulator of multiple nitrogen catabolic genes.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1180 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U27344; AAB03516.1; -; Genomic_DNA.
DR EMBL; D50617; BAA09217.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12419.1; -; Genomic_DNA.
DR PIR; S56233; S56233.
DR RefSeq; NP_116632.1; NM_001179945.1.
DR AlphaFoldDB; P43574; -.
DR SMR; P43574; -.
DR BioGRID; 31125; 79.
DR DIP; DIP-4276N; -.
DR IntAct; P43574; 7.
DR MINT; P43574; -.
DR STRING; 4932.YFL021W; -.
DR iPTMnet; P43574; -.
DR MaxQB; P43574; -.
DR PaxDb; P43574; -.
DR PRIDE; P43574; -.
DR EnsemblFungi; YFL021W_mRNA; YFL021W; YFL021W.
DR GeneID; 850523; -.
DR KEGG; sce:YFL021W; -.
DR SGD; S000001873; GAT1.
DR VEuPathDB; FungiDB:YFL021W; -.
DR eggNOG; KOG1601; Eukaryota.
DR HOGENOM; CLU_534434_0_0_1; -.
DR InParanoid; P43574; -.
DR BioCyc; YEAST:G3O-30439-MON; -.
DR Reactome; R-SCE-9018519; Estrogen-dependent gene expression.
DR PRO; PR:P43574; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P43574; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IMP:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IGI:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0090294; P:nitrogen catabolite activation of transcription; IMP:SGD.
DR GO; GO:0001080; P:nitrogen catabolite activation of transcription from RNA polymerase II promoter; IMP:SGD.
DR GO; GO:0010508; P:positive regulation of autophagy; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0036278; P:positive regulation of transcription from RNA polymerase II promoter in response to nitrogen starvation; IMP:SGD.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR013860; AreA_GATA.
DR InterPro; IPR039355; Transcription_factor_GATA.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR10071; PTHR10071; 1.
DR Pfam; PF08550; DUF1752; 1.
DR Pfam; PF00320; GATA; 1.
DR PRINTS; PR00619; GATAZNFINGER.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS00344; GATA_ZN_FINGER_1; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..510
FT /note="Transcriptional regulatory protein GAT1"
FT /id="PRO_0000083484"
FT ZN_FING 310..334
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 220..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 358..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 220..271
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 286..311
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 360..381
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 167
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 262
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 270
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 369
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 399
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 418
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956"
SQ SEQUENCE 510 AA; 56328 MW; 62D805E42695F35F CRC64;
MHVFFPLLFR PSPVLFIACA YIYIDIYIHC TRCTVVNITM STNRVPNLDP DLNLNKEIWD
LYSSAQKILP DSNRILNLSW RLHNRTSFHR INRIMQHSNS IMDFSASPFA SGVNAAGPGN
NDLDDTDTDN QQFFLSDMNL NGSSVFENVF DDDDDDDDVE THSIVHSDLL NDMDSASQRA
SHNASGFPNF LDTSCSSSFD DHFIFTNNLP FLNNNSINNN HSHNSSHNNN SPSIANNTNA
NTNTNTSAST NTNSPLLRRN PSPSIVKPGS RRNSSVRKKK PALKKIKSST SVQSSATPPS
NTSSNPDIKC SNCTTSTTPL WRKDPKGLPL CNACGLFLKL HGVTRPLSLK TDIIKKRQRS
STKINNNITP PPSSSLNPGA AGKKKNYTAS VAASKRKNSL NIVAPLKSQD IPIPKIASPS
IPQYLRSNTR HHLSSSVPIE AETFSSFRPD MNMTMNMNLH NASTSSFNNE AFWKPLDSAI
DHHSGDTNPN SNMNTTPNGN LSLDWLNLNL
