GAT25_ARATH
ID GAT25_ARATH Reviewed; 309 AA.
AC Q9LRH6; Q0WNU9; Q9STU8;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 2.
DT 25-MAY-2022, entry version 131.
DE RecName: Full=GATA transcription factor 25;
DE AltName: Full=Protein TIFY 1;
DE AltName: Full=Protein ZIM;
GN Name=GATA25; Synonyms=TIFY1, ZIM; OrderedLocusNames=At4g24470;
GN ORFNames=T22A6.300;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), SUBCELLULAR LOCATION,
RP AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=10945256; DOI=10.1271/bbb.64.1402;
RA Nishii A., Takemura M., Fujita H., Shikata M., Yokota A., Kohchi T.;
RT "Characterization of a novel gene encoding a putative single zinc-finger
RT protein, ZIM, expressed during the reproductive phase in Arabidopsis
RT thaliana.";
RL Biosci. Biotechnol. Biochem. 64:1402-1409(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=14966217; DOI=10.1093/jxb/erh078;
RA Shikata M., Matsuda Y., Ando K., Nishii A., Takemura M., Yokota A.,
RA Kohchi T.;
RT "Characterization of Arabidopsis ZIM, a member of a novel plant-specific
RT GATA factor gene family.";
RL J. Exp. Bot. 55:631-639(2004).
RN [7]
RP GENE FAMILY ORGANIZATION.
RX PubMed=15084732; DOI=10.1104/pp.103.037788;
RA Reyes J.C., Muro-Pastor M.I., Florencio F.J.;
RT "The GATA family of transcription factors in Arabidopsis and rice.";
RL Plant Physiol. 134:1718-1732(2004).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17499004; DOI=10.1016/j.tplants.2007.04.004;
RA Vanholme B., Grunewald W., Bateman A., Kohchi T., Gheysen G.;
RT "The tify family previously known as ZIM.";
RL Trends Plant Sci. 12:239-244(2007).
CC -!- FUNCTION: Transcriptional activator that specifically binds 5'-GATA-3'
CC or 5'-GAT-3' motifs within gene promoters. {ECO:0000250,
CC ECO:0000269|PubMed:14966217}.
CC -!- INTERACTION:
CC Q9LRH6; A0A178UXI0: At4g00780; NbExp=3; IntAct=EBI-2460434, EBI-25517350;
CC Q9LRH6; Q9ZVC9: FRS3; NbExp=4; IntAct=EBI-2460434, EBI-4448729;
CC Q9LRH6; Q9SMN1: GAMMACAL2; NbExp=3; IntAct=EBI-2460434, EBI-532001;
CC Q9LRH6; Q8GXL7: GATA24; NbExp=13; IntAct=EBI-2460434, EBI-4426127;
CC Q9LRH6; Q9LRH6: GATA25; NbExp=4; IntAct=EBI-2460434, EBI-2460434;
CC Q9LRH6; Q8H1G0: GATA28; NbExp=9; IntAct=EBI-2460434, EBI-4435064;
CC Q9LRH6; F4JL11: IMPA2; NbExp=3; IntAct=EBI-2460434, EBI-1253508;
CC Q9LRH6; Q8LCG7: NFYC2; NbExp=3; IntAct=EBI-2460434, EBI-2125983;
CC Q9LRH6; Q9FMV5: NFYC4; NbExp=3; IntAct=EBI-2460434, EBI-2466018;
CC Q9LRH6; Q84JF5: PTAC14; NbExp=3; IntAct=EBI-2460434, EBI-7890317;
CC Q9LRH6; Q9MAH8: TCP3; NbExp=3; IntAct=EBI-2460434, EBI-25522447;
CC Q9LRH6; Q9FEE2: TON2; NbExp=3; IntAct=EBI-2460434, EBI-4452426;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00357,
CC ECO:0000269|PubMed:10945256}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9LRH6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LRH6-2; Sequence=VSP_027849, VSP_027850;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in shoot apices,
CC inflorescences and roots. {ECO:0000269|PubMed:10945256,
CC ECO:0000269|PubMed:14966217}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the type IV zinc-finger family. Class C
CC subfamily. {ECO:0000305}.
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DR EMBL; AB035310; BAA97678.1; -; mRNA.
DR EMBL; AB035311; BAA97679.1; -; Genomic_DNA.
DR EMBL; AL078637; CAB45082.1; -; Genomic_DNA.
DR EMBL; AL161561; CAB79357.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84909.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84910.1; -; Genomic_DNA.
DR EMBL; AY086038; AAM63248.1; -; mRNA.
DR EMBL; AK229337; BAF01200.1; -; mRNA.
DR PIR; JC7336; JC7336.
DR PIR; T09910; T09910.
DR RefSeq; NP_194178.1; NM_118580.4. [Q9LRH6-1]
DR RefSeq; NP_849435.1; NM_179104.3. [Q9LRH6-1]
DR AlphaFoldDB; Q9LRH6; -.
DR SMR; Q9LRH6; -.
DR BioGRID; 13838; 19.
DR IntAct; Q9LRH6; 18.
DR STRING; 3702.AT4G24470.3; -.
DR PaxDb; Q9LRH6; -.
DR ProteomicsDB; 228963; -. [Q9LRH6-1]
DR EnsemblPlants; AT4G24470.1; AT4G24470.1; AT4G24470. [Q9LRH6-1]
DR EnsemblPlants; AT4G24470.2; AT4G24470.2; AT4G24470. [Q9LRH6-1]
DR GeneID; 828549; -.
DR Gramene; AT4G24470.1; AT4G24470.1; AT4G24470. [Q9LRH6-1]
DR Gramene; AT4G24470.2; AT4G24470.2; AT4G24470. [Q9LRH6-1]
DR KEGG; ath:AT4G24470; -.
DR Araport; AT4G24470; -.
DR eggNOG; KOG1601; Eukaryota.
DR InParanoid; Q9LRH6; -.
DR OMA; PMEGANQ; -.
DR PhylomeDB; Q9LRH6; -.
DR PRO; PR:Q9LRH6; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9LRH6; baseline and differential.
DR Genevisible; Q9LRH6; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00202; ZnF_GATA; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR010402; CCT_domain.
DR InterPro; IPR045280; TIFY-like.
DR InterPro; IPR010399; Tify_dom.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR46125; PTHR46125; 1.
DR Pfam; PF06203; CCT; 1.
DR Pfam; PF00320; GATA; 1.
DR Pfam; PF06200; tify; 1.
DR SMART; SM00979; TIFY; 1.
DR SMART; SM00401; ZnF_GATA; 1.
DR PROSITE; PS51017; CCT; 1.
DR PROSITE; PS00344; GATA_ZN_FINGER_1; 1.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 1.
DR PROSITE; PS51320; TIFY; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..309
FT /note="GATA transcription factor 25"
FT /id="PRO_0000083455"
FT DOMAIN 77..112
FT /note="Tify"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00650"
FT DOMAIN 146..188
FT /note="CCT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00357"
FT ZN_FING 208..267
FT /note="GATA-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 187..207
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 290..309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 187..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 178..182
FT /note="MARNK -> SVCQS (in isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_027849"
FT VAR_SEQ 183..309
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_027850"
FT CONFLICT 305
FT /note="L -> M (in Ref. 1; BAA97678)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 309 AA; 33261 MW; DA80FFC90A6AACB5 CRC64;
MFGRHSIIPN NQIGTASASA GEDHVSASAT SGHIPYDDME EIPHPDSIYG AASDLIPDGS
QLVAHRSDGS ELLVSRPPEG ANQLTISFRG QVYVFDAVGA DKVDAVLSLL GGSTELAPGP
QVMELAQQQN HMPVVEYQSR CSLPQRAQSL DRFRKKRNAR CFEKKVRYGV RQEVALRMAR
NKGQFTSSKM TDGAYNSGTD QDSAQDDAHP EISCTHCGIS SKCTPMMRRG PSGPRTLCNA
CGLFWANRGT LRDLSKKTEE NQLALMKPDD GGSVADAANN LNTEAASVEE HTSMVSLANG
DNSNLLGDH
