GAT2_ARATH
ID GAT2_ARATH Reviewed; 452 AA.
AC Q8L4X4; Q8LDF5; Q9FJ34;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Probable GABA transporter 2;
GN OrderedLocusNames=At5g41800; ORFNames=K16L22.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. La-0;
RX PubMed=14506206; DOI=10.1074/mcp.t300006-mcp200;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Large-scale analysis of in vivo phosphorylated membrane proteins by
RT immobilized metal ion affinity chromatography and mass spectrometry.";
RL Mol. Cell. Proteomics 2:1234-1243(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=15308754; DOI=10.1105/tpc.104.023150;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Phosphoproteomics of the Arabidopsis plasma membrane and a new
RT phosphorylation site database.";
RL Plant Cell 16:2394-2405(2004).
CC -!- FUNCTION: May be involved in the transport of GABA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10655.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB016871; BAB10655.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED94730.1; -; Genomic_DNA.
DR EMBL; AY099685; AAM20536.1; -; mRNA.
DR EMBL; AY128864; AAM91264.1; -; mRNA.
DR EMBL; AY086027; AAM63237.1; -; mRNA.
DR RefSeq; NP_568597.1; NM_123545.4.
DR AlphaFoldDB; Q8L4X4; -.
DR BioGRID; 19436; 33.
DR IntAct; Q8L4X4; 33.
DR STRING; 3702.AT5G41800.1; -.
DR iPTMnet; Q8L4X4; -.
DR PaxDb; Q8L4X4; -.
DR PRIDE; Q8L4X4; -.
DR ProteomicsDB; 230444; -.
DR EnsemblPlants; AT5G41800.1; AT5G41800.1; AT5G41800.
DR GeneID; 834185; -.
DR Gramene; AT5G41800.1; AT5G41800.1; AT5G41800.
DR KEGG; ath:AT5G41800; -.
DR Araport; AT5G41800; -.
DR TAIR; locus:2152995; AT5G41800.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_031160_1_0_1; -.
DR InParanoid; Q8L4X4; -.
DR OMA; CAFMAAM; -.
DR OrthoDB; 570025at2759; -.
DR PhylomeDB; Q8L4X4; -.
DR PRO; PR:Q8L4X4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8L4X4; baseline and differential.
DR Genevisible; Q8L4X4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..452
FT /note="Probable GABA transporter 2"
FT /id="PRO_0000418999"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 17
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:14506206,
FT ECO:0007744|PubMed:15308754"
FT CONFLICT 64
FT /note="V -> F (in Ref. 4; AAM63237)"
FT /evidence="ECO:0000305"
FT CONFLICT 180
FT /note="C -> F (in Ref. 4; AAM63237)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 452 AA; 49856 MW; 3A24EA9F0A24830D CRC64;
MTDPPRPDPF PVTRLDSDAG ALFVLQSKGE WWHAGFHLTT AIVGPTILTL PYAFRGLGWW
LGFVCLTTMG LVTFYAYYLM SKVLDHCEKS GRRHIRFREL AADVLGSGLM FYVVIFIQTA
INTGIGIGAI LLAGQCLDIM YSSLFPQGTL KLYEFIAMVT VVMMVLSQLP SFHSLRHINC
ASLLLSLGYT FLVVGACINL GLSKNAPKRE YSLEHSDSGK VFSAFTSISI IAAIFGNGIL
PEIQATLAPP ATGKMLKGLL LCYSVIFFTF YSAAISGYWV FGNNSSSNIL KNLMPDEGPT
LAPIVVIGLA VIFVLLQLFA IGLVYSQVAY EIMEKKSADT TKGIFSKRNL VPRLILRTLY
MAFCGFMAAM LPFFGDINAV VGAFGFIPLD FVLPMLLYNM TYKPTRRSFT YWINMTIMVV
FTCAGLMGAF SSIRKLVLDA NKFKLFSSEV VD
