GATA4_RAT
ID GATA4_RAT Reviewed; 440 AA.
AC P46152;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Transcription factor GATA-4;
DE AltName: Full=DNA-binding protein GATA-GT2;
DE AltName: Full=GATA-binding factor 4;
GN Name=Gata4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar; TISSUE=Stomach;
RX PubMed=8248184; DOI=10.1073/pnas.90.22.10876;
RA Tamura S., Wang X.H., Maeda M., Futai M.;
RT "Gastric DNA-binding proteins recognize upstream sequence motifs of
RT parietal cell-specific genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:10876-10880(1993).
RN [2]
RP ERRATUM OF PUBMED:8248184.
RX PubMed=8183957; DOI=10.1073/pnas.91.10.4609;
RA Tamura S., Wang X.H., Maeda M., Futai M.;
RL Proc. Natl. Acad. Sci. U.S.A. 91:4609-4609(1994).
RN [3]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=15329343; DOI=10.1242/dev.01344;
RA Lee K.H., Evans S., Ruan T.Y., Lassar A.B.;
RT "SMAD-mediated modulation of YY1 activity regulates the BMP response and
RT cardiac-specific expression of a GATA4/5/6-dependent chick Nkx2.5
RT enhancer.";
RL Development 131:4709-4723(2004).
RN [4]
RP INTERACTION WITH ZNF260.
RX PubMed=16166646; DOI=10.1128/mcb.25.19.8669-8682.2005;
RA Debrus S., Rahbani L., Marttila M., Delorme B., Paradis P., Nemer M.;
RT "The zinc finger-only protein Zfp260 is a novel cardiac regulator and a
RT nuclear effector of alpha1-adrenergic signaling.";
RL Mol. Cell. Biol. 25:8669-8682(2005).
CC -!- FUNCTION: Transcriptional activator that binds to the consensus
CC sequence 5'-AGATAG-3' and plays a key role in cardiac development (By
CC similarity). In cooperation with TBX5, it binds to cardiac super-
CC enhancers and promotes cardiomyocyte gene expression, while it down-
CC regulates endocardial and endothelial gene expression (By similarity).
CC Acts as a transcriptional activator of ANF in cooperation with NKX2-5
CC (By similarity). Promotes cardiac myocyte enlargement (By similarity).
CC Required during testicular development (By similarity). Involved in
CC bone morphogenetic protein (BMP)-mediated induction of cardiac-specific
CC gene expression (PubMed:15329343). Binds to BMP response element
CC (BMPRE) DNA sequences within cardiac activating regions
CC (PubMed:15329343). May play a role in sphingolipid signaling by
CC regulating the expression of sphingosine-1-phosphate degrading enzyme,
CC sphingosine-1-phosphate lyase (By similarity).
CC {ECO:0000250|UniProtKB:P43694, ECO:0000250|UniProtKB:Q08369,
CC ECO:0000269|PubMed:15329343}.
CC -!- SUBUNIT: Interacts with the homeobox domain of NKX2-5 through its C-
CC terminal zinc finger. Also interacts with JARID2 which represses its
CC ability to activate transcription of ANF. Interacts (via the second Zn
CC finger) with NFATC4 (By similarity). Interacts with LMCD1 (By
CC similarity). Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and
CC GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with
CC NR5A1, ZFPM2 and TBX5. Interacts with ZNF260. Interacts with TBX18.
CC {ECO:0000250|UniProtKB:P43694, ECO:0000250|UniProtKB:Q08369,
CC ECO:0000269|PubMed:16166646}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P43694}.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in gastric mucosa and at
CC much lower levels in the intestine. Also expressed in testis.
CC -!- PTM: Methylation at Lys-299 attenuates transcriptional activity.
CC {ECO:0000250}.
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DR EMBL; L22761; AAA16159.1; -; mRNA.
DR PIR; I61183; I61183.
DR RefSeq; NP_653331.1; NM_144730.1.
DR AlphaFoldDB; P46152; -.
DR SMR; P46152; -.
DR BioGRID; 248477; 4.
DR DIP; DIP-60791N; -.
DR IntAct; P46152; 3.
DR MINT; P46152; -.
DR STRING; 10116.ENSRNOP00000014320; -.
DR ChEMBL; CHEMBL4105727; -.
DR iPTMnet; P46152; -.
DR PhosphoSitePlus; P46152; -.
DR PaxDb; P46152; -.
DR PRIDE; P46152; -.
DR Ensembl; ENSRNOT00000014320; ENSRNOP00000014320; ENSRNOG00000010708.
DR GeneID; 54254; -.
DR KEGG; rno:54254; -.
DR UCSC; RGD:2665; rat.
DR CTD; 2626; -.
DR RGD; 2665; Gata4.
DR eggNOG; KOG1601; Eukaryota.
DR GeneTree; ENSGT00940000158349; -.
DR HOGENOM; CLU_027524_0_0_1; -.
DR InParanoid; P46152; -.
DR OMA; GIMTSNH; -.
DR OrthoDB; 807790at2759; -.
DR PhylomeDB; P46152; -.
DR Reactome; R-RNO-983231; Factors involved in megakaryocyte development and platelet production.
DR PRO; PR:P46152; -.
DR Proteomes; UP000002494; Chromosome 15.
DR Bgee; ENSRNOG00000010708; Expressed in stomach and 10 other tissues.
DR Genevisible; P46152; RN.
DR GO; GO:0000785; C:chromatin; ISO:RGD.
DR GO; GO:0016604; C:nuclear body; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; ISO:RGD.
DR GO; GO:0003682; F:chromatin binding; ISO:RGD.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0070410; F:co-SMAD binding; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; IDA:BHF-UCL.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; ISO:RGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:BHF-UCL.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:UniProtKB.
DR GO; GO:0051525; F:NFAT protein binding; IPI:RGD.
DR GO; GO:0019901; F:protein kinase binding; IPI:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:BHF-UCL.
DR GO; GO:0043565; F:sequence-specific DNA binding; IMP:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:BHF-UCL.
DR GO; GO:0001223; F:transcription coactivator binding; ISO:RGD.
DR GO; GO:0008270; F:zinc ion binding; TAS:RGD.
DR GO; GO:0003180; P:aortic valve morphogenesis; ISO:RGD.
DR GO; GO:0060413; P:atrial septum morphogenesis; ISO:RGD.
DR GO; GO:0003289; P:atrial septum primum morphogenesis; ISO:RGD.
DR GO; GO:0003290; P:atrial septum secundum morphogenesis; ISO:RGD.
DR GO; GO:0036302; P:atrioventricular canal development; ISO:RGD.
DR GO; GO:0003190; P:atrioventricular valve formation; ISO:RGD.
DR GO; GO:0003181; P:atrioventricular valve morphogenesis; ISO:RGD.
DR GO; GO:0055007; P:cardiac muscle cell differentiation; IEP:RGD.
DR GO; GO:0014898; P:cardiac muscle hypertrophy in response to stress; ISO:RGD.
DR GO; GO:0048738; P:cardiac muscle tissue development; ISO:RGD.
DR GO; GO:0061026; P:cardiac muscle tissue regeneration; ISO:RGD.
DR GO; GO:0003215; P:cardiac right ventricle morphogenesis; ISO:RGD.
DR GO; GO:0003279; P:cardiac septum development; ISO:RGD.
DR GO; GO:0045165; P:cell fate commitment; IBA:GO_Central.
DR GO; GO:0061049; P:cell growth involved in cardiac muscle cell development; IDA:RGD.
DR GO; GO:0007267; P:cell-cell signaling; ISO:RGD.
DR GO; GO:0071372; P:cellular response to follicle-stimulating hormone stimulus; ISO:RGD.
DR GO; GO:0071333; P:cellular response to glucose stimulus; IEP:RGD.
DR GO; GO:0071371; P:cellular response to gonadotropin stimulus; ISO:RGD.
DR GO; GO:0048557; P:embryonic digestive tract morphogenesis; ISO:RGD.
DR GO; GO:0048617; P:embryonic foregut morphogenesis; ISO:RGD.
DR GO; GO:0035054; P:embryonic heart tube anterior/posterior pattern specification; ISO:RGD.
DR GO; GO:0035050; P:embryonic heart tube development; ISO:RGD.
DR GO; GO:0048598; P:embryonic morphogenesis; ISO:RGD.
DR GO; GO:0003197; P:endocardial cushion development; ISO:RGD.
DR GO; GO:0001706; P:endoderm formation; ISO:RGD.
DR GO; GO:0072148; P:epithelial cell fate commitment; ISO:RGD.
DR GO; GO:0001702; P:gastrulation with mouth forming second; ISO:RGD.
DR GO; GO:0007507; P:heart development; ISO:RGD.
DR GO; GO:0001947; P:heart looping; ISO:RGD.
DR GO; GO:0003007; P:heart morphogenesis; ISO:RGD.
DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR GO; GO:0060575; P:intestinal epithelial cell differentiation; ISO:RGD.
DR GO; GO:0060464; P:lung lobe formation; ISO:RGD.
DR GO; GO:0008584; P:male gonad development; ISO:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD.
DR GO; GO:2001234; P:negative regulation of apoptotic signaling pathway; ISO:RGD.
DR GO; GO:0010507; P:negative regulation of autophagy; IMP:RGD.
DR GO; GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; ISO:RGD.
DR GO; GO:1905204; P:negative regulation of connective tissue replacement; ISO:RGD.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:RGD.
DR GO; GO:1903202; P:negative regulation of oxidative stress-induced cell death; ISO:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045766; P:positive regulation of angiogenesis; ISO:RGD.
DR GO; GO:0030513; P:positive regulation of BMP signaling pathway; IDA:BHF-UCL.
DR GO; GO:0060045; P:positive regulation of cardiac muscle cell proliferation; ISO:RGD.
DR GO; GO:0051891; P:positive regulation of cardioblast differentiation; ISO:RGD.
DR GO; GO:0045787; P:positive regulation of cell cycle; ISO:RGD.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0010575; P:positive regulation of vascular endothelial growth factor production; ISO:RGD.
DR GO; GO:0086004; P:regulation of cardiac muscle cell contraction; IMP:RGD.
DR GO; GO:0060043; P:regulation of cardiac muscle cell proliferation; ISO:RGD.
DR GO; GO:0010468; P:regulation of gene expression; ISO:RGD.
DR GO; GO:0051896; P:regulation of protein kinase B signaling; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:RGD.
DR GO; GO:0043627; P:response to estrogen; ISO:RGD.
DR GO; GO:0009612; P:response to mechanical stimulus; IDA:RGD.
DR GO; GO:0032526; P:response to retinoic acid; ISO:RGD.
DR GO; GO:0033189; P:response to vitamin A; IEP:RGD.
DR GO; GO:0009410; P:response to xenobiotic stimulus; ISO:RGD.
DR GO; GO:0072520; P:seminiferous tubule development; ISO:RGD.
DR GO; GO:0060008; P:Sertoli cell differentiation; ISO:RGD.
DR GO; GO:0023019; P:signal transduction involved in regulation of gene expression; ISO:RGD.
DR GO; GO:0060395; P:SMAD protein signal transduction; ISO:RGD.
DR GO; GO:0007283; P:spermatogenesis; ISO:RGD.
DR GO; GO:0060290; P:transdifferentiation; IMP:RGD.
DR GO; GO:0035239; P:tube morphogenesis; ISO:RGD.
DR GO; GO:0003229; P:ventricular cardiac muscle tissue development; ISO:RGD.
DR GO; GO:0003281; P:ventricular septum development; ISO:RGD.
DR GO; GO:0042060; P:wound healing; ISO:RGD.
DR CDD; cd00202; ZnF_GATA; 2.
DR Gene3D; 3.30.50.10; -; 2.
DR InterPro; IPR008013; GATA_N.
DR InterPro; IPR016375; TF_GATA_4/5/6.
DR InterPro; IPR039355; Transcription_factor_GATA.
DR InterPro; IPR000679; Znf_GATA.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR PANTHER; PTHR10071; PTHR10071; 1.
DR Pfam; PF00320; GATA; 2.
DR Pfam; PF05349; GATA-N; 1.
DR PIRSF; PIRSF003028; TF_GATA_4/5/6; 1.
DR PRINTS; PR00619; GATAZNFINGER.
DR SMART; SM00401; ZnF_GATA; 2.
DR PROSITE; PS00344; GATA_ZN_FINGER_1; 2.
DR PROSITE; PS50114; GATA_ZN_FINGER_2; 2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Metal-binding; Methylation; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..440
FT /note="Transcription factor GATA-4"
FT /id="PRO_0000083415"
FT ZN_FING 216..240
FT /note="GATA-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT ZN_FING 270..294
FT /note="GATA-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00094"
FT REGION 60..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 313..392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 404..440
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 60..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 339..362
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 371..392
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 406..429
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 299
FT /note="N6-methyllysine; by EZH2"
FT /evidence="ECO:0000250|UniProtKB:Q08369"
SQ SEQUENCE 440 AA; 44597 MW; 7C594FA95080AD43 CRC64;
MYQSLAMAAN HGPPPGAYEA GGPGAFMHSA GAASSPVYVP TPRVPSSVLG LSYLQGGGSG
AASGATSGGS SGAGPSGAGP GTQQGSPGWS QAGAEGAAYT PPPVSPRFSF PGTTGSLAAA
AAAAAAREAA AYSSSGGAAG AGLAGREQYG RPGFAGSYSS PYPAYMADVG ASWAAAAAAS
AGPFDSPVLH SLPGRANPAR HPNLDMFDDF SEGRECVNCG AMSTPLWRRD GTGHYLCNAC
GLYHKMNGIN RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL
HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPPGESLPP SSGASSNSSN ATSSSSSSEE
MRPIKTEPGL SSHYGHSSSM SQTFSTVSGH GSSIHPVLSA LKLSPQGYPS PVTQTSQASS
KQDSWNSLVL ADSHGDIITA
