GATA_AQUAE
ID GATA_AQUAE Reviewed; 478 AA.
AC O66610;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Glutamyl-tRNA(Gln) amidotransferase subunit A;
DE Short=Glu-ADT subunit A;
DE EC=6.3.5.7;
GN Name=gatA; OrderedLocusNames=aq_247;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
CC -!- FUNCTION: Allows the formation of correctly charged Gln-tRNA(Gln)
CC through the transamidation of misacylated Glu-tRNA(Gln) in organisms
CC which lack glutaminyl-tRNA synthetase. The reaction takes place in the
CC presence of glutamine and ATP through an activated gamma-phospho-Glu-
CC tRNA(Gln) (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) +
CC L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate;
CC Xref=Rhea:RHEA:17521, Rhea:RHEA-COMP:9681, Rhea:RHEA-COMP:9684,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359,
CC ChEBI:CHEBI:78520, ChEBI:CHEBI:78521, ChEBI:CHEBI:456216; EC=6.3.5.7;
CC -!- SUBUNIT: Heterotrimer of A, B and C subunits. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the amidase family. GatA subfamily.
CC {ECO:0000305}.
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DR EMBL; AE000657; AAC06569.1; -; Genomic_DNA.
DR PIR; F70322; F70322.
DR RefSeq; NP_213170.1; NC_000918.1.
DR RefSeq; WP_010880108.1; NC_000918.1.
DR PDB; 3H0L; X-ray; 2.30 A; A/D/G/J/M/P/S/V=1-478.
DR PDB; 3H0M; X-ray; 2.80 A; A/D/G/J/M/P/S/V=1-478.
DR PDB; 3H0R; X-ray; 3.00 A; A/D/G/J/M/P/S/V=1-478.
DR PDBsum; 3H0L; -.
DR PDBsum; 3H0M; -.
DR PDBsum; 3H0R; -.
DR AlphaFoldDB; O66610; -.
DR SMR; O66610; -.
DR STRING; 224324.aq_247; -.
DR PRIDE; O66610; -.
DR EnsemblBacteria; AAC06569; AAC06569; aq_247.
DR KEGG; aae:aq_247; -.
DR PATRIC; fig|224324.8.peg.202; -.
DR eggNOG; COG0154; Bacteria.
DR HOGENOM; CLU_009600_0_3_0; -.
DR InParanoid; O66610; -.
DR OMA; EVSCPHF; -.
DR OrthoDB; 1239251at2; -.
DR EvolutionaryTrace; O66610; -.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0030956; C:glutamyl-tRNA(Gln) amidotransferase complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0050567; F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.1300.10; -; 1.
DR HAMAP; MF_00120; GatA; 1.
DR InterPro; IPR000120; Amidase.
DR InterPro; IPR020556; Amidase_CS.
DR InterPro; IPR023631; Amidase_dom.
DR InterPro; IPR036928; AS_sf.
DR InterPro; IPR004412; GatA.
DR PANTHER; PTHR11895; PTHR11895; 1.
DR Pfam; PF01425; Amidase; 1.
DR SUPFAM; SSF75304; SSF75304; 1.
DR TIGRFAMs; TIGR00132; gatA; 1.
DR PROSITE; PS00571; AMIDASES; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Ligase; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..478
FT /note="Glutamyl-tRNA(Gln) amidotransferase subunit A"
FT /id="PRO_0000105133"
FT ACT_SITE 72
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 147
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 171
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000250"
FT HELIX 7..15
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 21..39
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 47..55
FT /evidence="ECO:0007829|PDB:3H0L"
FT TURN 63..66
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 68..72
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 87..89
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 99..106
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 110..115
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 119..121
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 124..126
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 146..148
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 149..156
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 159..161
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 163..166
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 168..170
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 173..179
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 182..185
FT /evidence="ECO:0007829|PDB:3H0L"
FT TURN 201..203
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 206..212
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 213..223
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 241..244
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 253..257
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 258..262
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 267..282
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 286..290
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 295..297
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 298..313
FT /evidence="ECO:0007829|PDB:3H0L"
FT TURN 314..316
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 318..323
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 332..343
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 346..358
FT /evidence="ECO:0007829|PDB:3H0L"
FT TURN 361..367
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 368..385
FT /evidence="ECO:0007829|PDB:3H0L"
FT TURN 386..388
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 390..396
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 398..400
FT /evidence="ECO:0007829|PDB:3H0L"
FT TURN 404..407
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 408..410
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 411..415
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 416..418
FT /evidence="ECO:0007829|PDB:3H0L"
FT TURN 419..421
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 422..427
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 431..439
FT /evidence="ECO:0007829|PDB:3H0L"
FT STRAND 442..450
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 455..468
FT /evidence="ECO:0007829|PDB:3H0L"
FT HELIX 471..473
FT /evidence="ECO:0007829|PDB:3H0L"
SQ SEQUENCE 478 AA; 53524 MW; 72EE383259D606E6 CRC64;
MLWKKSLSEL RELLKRGEVS PKEVVESFYD RYNQTEEKVK AYITPLYGKA LKQAESLKER
ELPLFGIPIA VKDNILVEGE KTTCASKILE NFVAPYDATV IERLKKAGAL IVGKTNLDEF
AMGSSTEYSA FFPTKNPWDL ERVPGGSSGG SAASVAVLSA PVSLGSDTGG SIRQPASFCG
VIGIKPTYGR VSRYGLVAFA SSLDQIGVFG RRTEDVALVL EVISGWDEKD STSAKVPVPE
WSEEVKKEVK GLKIGLPKEF FEYELQPQVK EAFENFIKEL EKEGFEIKEV SLPHVKYSIP
TYYIIAPSEA SSNLARYDGV RYGYRAKEYK DIFEMYARTR DEGFGPEVKR RIMLGTFALS
AGYYDAYYLK AQKVRRLITN DFLKAFEEVD VIASPTTPTL PFKFGERLEN PIEMYLSDIL
TVPANLAGLP AISIPIAWKD GLPVGGQLIG KHWDETTLLQ ISYLWEQKFK HYEKIPLT
