ID   ALIS2_ARATH             Reviewed;         343 AA.
AC   Q67YS6; Q0WQT3; Q67YX0; Q8LDM0; Q9FNK7;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Putative ALA-interacting subunit 2;
DE            Short=AtALIS2;
GN   Name=ALIS2; OrderedLocusNames=At5g46150; ORFNames=MCL19.21;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA   Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT   features of the regions of 1,044,062 bp covered by thirteen physically
RT   assigned P1 clones.";
RL   DNA Res. 4:291-300(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NOMENCLATURE, AND TISSUE SPECIFICITY.
RX   PubMed=18344284; DOI=10.1105/tpc.107.054767;
RA   Poulsen L.R., Lopez-Marques R.L., McDowell S.C., Okkeri J., Licht D.,
RA   Schulz A., Pomorski T., Harper J.F., Palmgren M.G.;
RT   "The Arabidopsis P4-ATPase ALA3 localizes to the Golgi and requires a beta-
RT   subunit to function in lipid translocation and secretory vesicle
RT   formation.";
RL   Plant Cell 20:658-676(2008).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC       siliques. {ECO:0000269|PubMed:18344284}.
CC   -!- SIMILARITY: Belongs to the CDC50/LEM3 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM63135.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAB08260.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB006698; BAB08260.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002688; AED95345.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED95346.1; -; Genomic_DNA.
DR   EMBL; AK175295; BAD43058.1; -; mRNA.
DR   EMBL; AK176392; BAD44155.1; -; mRNA.
DR   EMBL; AK176644; BAD44407.1; -; mRNA.
DR   EMBL; AK221710; BAD95435.1; -; mRNA.
DR   EMBL; AK228602; BAF00516.1; -; mRNA.
DR   EMBL; AK176348; BAD44111.1; -; mRNA.
DR   EMBL; AY085923; AAM63135.1; ALT_INIT; mRNA.
DR   EMBL; BT024877; ABD85148.1; -; mRNA.
DR   RefSeq; NP_001330145.1; NM_001344659.1.
DR   RefSeq; NP_568657.1; NM_123984.4.
DR   RefSeq; NP_851139.1; NM_180808.2.
DR   AlphaFoldDB; Q67YS6; -.
DR   SMR; Q67YS6; -.
DR   STRING; 3702.AT5G46150.2; -.
DR   iPTMnet; Q67YS6; -.
DR   PaxDb; Q67YS6; -.
DR   PRIDE; Q67YS6; -.
DR   ProteomicsDB; 244809; -.
DR   EnsemblPlants; AT5G46150.1; AT5G46150.1; AT5G46150.
DR   EnsemblPlants; AT5G46150.2; AT5G46150.2; AT5G46150.
DR   GeneID; 834657; -.
DR   Gramene; AT5G46150.1; AT5G46150.1; AT5G46150.
DR   Gramene; AT5G46150.2; AT5G46150.2; AT5G46150.
DR   KEGG; ath:AT5G46150; -.
DR   Araport; AT5G46150; -.
DR   TAIR; locus:2161453; AT5G46150.
DR   eggNOG; KOG2952; Eukaryota.
DR   HOGENOM; CLU_025025_1_1_1; -.
DR   InParanoid; Q67YS6; -.
DR   OrthoDB; 889671at2759; -.
DR   PhylomeDB; Q67YS6; -.
DR   PRO; PR:Q67YS6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q67YS6; baseline and differential.
DR   Genevisible; Q67YS6; AT.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0033036; P:macromolecule localization; IEA:UniProt.
DR   InterPro; IPR005045; CDC50/LEM3_fam.
DR   PANTHER; PTHR10926; PTHR10926; 1.
DR   Pfam; PF03381; CDC50; 1.
DR   PIRSF; PIRSF015840; DUF284_TM_euk; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..343
FT                   /note="Putative ALA-interacting subunit 2"
FT                   /id="PRO_0000366955"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        301..321
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        103
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        178
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        191
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        218
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        119
FT                   /note="F -> L (in Ref. 3; BAF00516)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        231
FT                   /note="I -> L (in Ref. 4; AAM63135)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        257
FT                   /note="R -> G (in Ref. 3; BAD44111)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        314
FT                   /note="I -> V (in Ref. 4; AAM63135)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   343 AA;  39546 MW;  12CA7D9B4A73A985 CRC64;
     MMEVEGSMNR APDQSSFLRS RRSKALYQFK QQKLPACKPV LTPISVITVF MLMGFVFIPI
     GLITLRASRD AIEIIDRYDV ECIPEEYRTN KLLYITDSSI PKNCTRYLKV QKYMKAPIFI
     YYQLDNYYQN HRRYVKSRSD QQLLHGLEYS HTSSCEPEES SNGLPIVPCG LIAWSMFNDT
     FTFSRERTKL NVSRNNIAWK SDREHKFGKN VYPINFQNGT LIGGAKLDPK IPLSDQEDFI
     VWMRAAALLS FRKLYGRIEE DLEPGKVVEV NLMNNYNTYS FSGQKKLILS TSNWLGGRND
     FLGITYLVVG SSSIVISIIF MLLHLKNPRP YGDNSWNKKS LSS