ID   ALIX_DICDI              Reviewed;         794 AA.
AC   Q8T7K0; Q552W2;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=ALG-2 interacting protein X;
DE            Short=Alix;
DE            Short=Dd-Alix;
DE            Short=DdAlix;
GN   Name=alxA; Synonyms=alx; ORFNames=DDB_G0275451;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=15708561; DOI=10.1016/j.ydbio.2004.12.004;
RA   Mattei S., Ryves W.J., Blot B., Sadoul R., Harwood A.J., Satre M.,
RA   Klein G., Aubry L.;
RT   "Dd-Alix, a conserved endosome-associated protein, controls Dictyostelium
RT   development.";
RL   Dev. Biol. 279:99-113(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=15276209; DOI=10.1016/j.gene.2004.04.020;
RA   Ohkouchi S., El-Halawany M.S., Aruga F., Shibata H., Hitomi K., Maki M.;
RT   "DdAlix, an Alix/AIP1 homolog in Dictyostelium discoideum, is required for
RT   multicellular development under low Ca2+ conditions.";
RL   Gene 337:131-139(2004).
RN   [5]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=15757670; DOI=10.1016/j.febslet.2005.02.036;
RA   Ohkouchi S., Saito H., Aruga F., Maeda T., Shibata H., Maki M.;
RT   "Dictyostelium discoideum requires an Alix/AIP1 homolog, DdAlix, for
RT   morphogenesis in alkaline environments.";
RL   FEBS Lett. 579:1745-1750(2005).
RN   [6]
RP   SELF-ASSOCIATION, AND INTERACTION WITH PEFA AND PEFB.
RX   PubMed=16766083; DOI=10.1016/j.ejcb.2006.04.002;
RA   Mattei S., Klein G., Satre M., Aubry L.;
RT   "Trafficking and developmental signaling: Alix at the crossroads.";
RL   Eur. J. Cell Biol. 85:925-936(2006).
CC   -!- FUNCTION: Unknown. Required for development but not for cell death.
CC   -!- SUBUNIT: Self-associates; the interaction is calcium-independent
CC       Interacts with pefa; the interaction is calcium-dependent. Interacts
CC       with pefb; the interaction is calcium-dependent.
CC       {ECO:0000269|PubMed:16766083}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15708561}.
CC       Cytoplasmic vesicle membrane {ECO:0000269|PubMed:15708561}. Endosome
CC       {ECO:0000269|PubMed:15708561}. Note=Colocalizes to large vesicle-like
CC       structures with chmp4. Colocalizes with vamp7A to endosomes but not to
CC       lysosomes.
CC   -!- DISRUPTION PHENOTYPE: No obvious effect on unicellular growth but
CC       induces a premature development arrest, with an inability to
CC       differentiate spores and to build a true stalk indicative of a major
CC       defect in differentiation and morphogenesis. The abortive structure
CC       contains a mixture of undifferentiated cells and vacuolated stalk-like
CC       cells. Differing results include abnormal development only at very low
CC       extracellular Ca(2+) conditions (below 10 nM) (PubMed:15276209) or at
CC       alkaline pH (pH 9) (PubMed:15757670). {ECO:0000269|PubMed:15276209,
CC       ECO:0000269|PubMed:15708561, ECO:0000269|PubMed:15757670}.
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DR   EMBL; AF360741; AAM00241.1; -; Genomic_DNA.
DR   EMBL; AAFI02000013; EAL69476.1; -; Genomic_DNA.
DR   RefSeq; XP_643566.1; XM_638474.1.
DR   AlphaFoldDB; Q8T7K0; -.
DR   SMR; Q8T7K0; -.
DR   STRING; 44689.DDB0185177; -.
DR   PaxDb; Q8T7K0; -.
DR   ABCD; Q8T7K0; 6 sequenced antibodies.
DR   EnsemblProtists; EAL69476; EAL69476; DDB_G0275451.
DR   GeneID; 8620152; -.
DR   KEGG; ddi:DDB_G0275451; -.
DR   dictyBase; DDB_G0275451; alxA.
DR   eggNOG; KOG2220; Eukaryota.
DR   HOGENOM; CLU_003661_0_0_1; -.
DR   InParanoid; Q8T7K0; -.
DR   OMA; SEWIHHM; -.
DR   PhylomeDB; Q8T7K0; -.
DR   Reactome; R-DDI-5213460; RIPK1-mediated regulated necrosis.
DR   PRO; PR:Q8T7K0; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005768; C:endosome; IDA:dictyBase.
DR   GO; GO:0009653; P:anatomical structure morphogenesis; IMP:dictyBase.
DR   GO; GO:0019722; P:calcium-mediated signaling; IMP:dictyBase.
DR   GO; GO:0031286; P:negative regulation of sorocarp stalk cell differentiation; IMP:dictyBase.
DR   GO; GO:0045881; P:positive regulation of sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR   GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IBA:GO_Central.
DR   Gene3D; 1.25.40.280; -; 1.
DR   InterPro; IPR025304; ALIX_V_dom.
DR   InterPro; IPR045251; BRO1-like.
DR   InterPro; IPR004328; BRO1_dom.
DR   InterPro; IPR038499; BRO1_sf.
DR   PANTHER; PTHR23030; PTHR23030; 1.
DR   Pfam; PF13949; ALIX_LYPXL_bnd; 1.
DR   Pfam; PF03097; BRO1; 1.
DR   SMART; SM01041; BRO1; 1.
DR   PROSITE; PS51180; BRO1; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Cytoplasmic vesicle; Endosome; Membrane;
KW   Reference proteome.
FT   CHAIN           1..794
FT                   /note="ALG-2 interacting protein X"
FT                   /id="PRO_0000375887"
FT   DOMAIN          1..385
FT                   /note="BRO1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00526"
FT   REGION          695..794
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          499..568
FT                   /evidence="ECO:0000255"
FT   COILED          600..655
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        697..745
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        746..784
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   794 AA;  90526 MW;  40BEAC3A4C4AA135 CRC64;
     MLSIERKRTE KVDFSKPLTK YIKEQFSKAE SDQHETQIAT LNGLREDVRN LQERTETSKE
     MVWKYYSILS SLELRFPISE NNVRISFPWT DSYRQRKSTL YSIYFERASV LFNYGSIVSQ
     IASSTNRSNI EGVKKACNQF QLAAGVFNKL REYASLHPEC STSADFSSES LQALVTIMLA
     QAQECIYEKA SMDNLSDSIL SKLAAQVAEY YDTFNQLLNS NSLKSIVDRN WNITATVKSY
     LYKAISLYCH AKGLEQVSQF GEQVSRLMIA VDNINQSKVN LAKTAPIELK EIVERYVISI
     TRYCESAKKD NDTIYHDTIP PAHKLTPIEK KPLAKALPLP EINFVDPFNS LVPFSVKEDS
     AYYNDQKETL LRKELDNIEF HNQSAKASLL SMGLPGSIEA LDVGVPVALK EKMNVVTNEQ
     GVSNITRLLE NIQQLSDEDS AICLSAGNLL KKEEDEDNLM RATYGAQWHR TPSYTLTANL
     TQDYAKYTSH LQHSTKSDSF IRKKFEDHKN SIQELENQTE IIALLPTNNL PSGKIAEIAS
     LTVLMNDLDA LMANRESIAE KLKNLCKKDD ITLKLLSPQK DKSLIYAEEI QKYEPLQMSL
     NESFLKQQKL IEDIRKENEK FTNQKSKQGN QREEILQKYA NAYKVYNELK ANLDEGTQFY
     LNFQEILNKF LNRCKDFTTG RENEKIELKR QIEAGVNPHS PLTSPSPSLQ SPVNNYPNQF
     SSPQYHTSPN QQQQQQQQYV PSSQPPPQYS YNPQPYQPPQ QFGGPLPPPQ SFSAPPPPQS
     FTAPPPYNSN NKHY