ALKB6_MOUSE
ID ALKB6_MOUSE Reviewed; 238 AA.
AC Q8K2U2; G3X994;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 3.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Alpha-ketoglutarate-dependent dioxygenase alkB homolog 6;
DE EC=1.14.11.-;
DE AltName: Full=Alkylated DNA repair protein alkB homolog 6;
GN Name=Alkbh6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable dioxygenase that requires molecular oxygen, alpha-
CC ketoglutarate and iron. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00805};
CC -!- SUBUNIT: Interacts with VCPKMT. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the alkB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH29805.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC149067; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466593; EDL24033.1; -; Genomic_DNA.
DR EMBL; BC029805; AAH29805.1; ALT_FRAME; mRNA.
DR CCDS; CCDS21085.1; -.
DR RefSeq; NP_932144.2; NM_198027.2.
DR AlphaFoldDB; Q8K2U2; -.
DR SMR; Q8K2U2; -.
DR STRING; 10090.ENSMUSP00000051515; -.
DR iPTMnet; Q8K2U2; -.
DR PhosphoSitePlus; Q8K2U2; -.
DR EPD; Q8K2U2; -.
DR MaxQB; Q8K2U2; -.
DR PaxDb; Q8K2U2; -.
DR PRIDE; Q8K2U2; -.
DR ProteomicsDB; 296195; -.
DR Antibodypedia; 68413; 77 antibodies from 17 providers.
DR Ensembl; ENSMUST00000060834; ENSMUSP00000051515; ENSMUSG00000042831.
DR GeneID; 233065; -.
DR KEGG; mmu:233065; -.
DR UCSC; uc009geb.1; mouse.
DR CTD; 84964; -.
DR MGI; MGI:2142037; Alkbh6.
DR VEuPathDB; HostDB:ENSMUSG00000042831; -.
DR eggNOG; KOG3200; Eukaryota.
DR GeneTree; ENSGT00510000048626; -.
DR InParanoid; Q8K2U2; -.
DR OMA; FESQNAN; -.
DR OrthoDB; 1573279at2759; -.
DR PhylomeDB; Q8K2U2; -.
DR TreeFam; TF314467; -.
DR BioGRID-ORCS; 233065; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Alkbh6; mouse.
DR PRO; PR:Q8K2U2; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8K2U2; protein.
DR Bgee; ENSMUSG00000042831; Expressed in adrenal gland and 61 other tissues.
DR ExpressionAtlas; Q8K2U2; baseline and differential.
DR Genevisible; Q8K2U2; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005925; C:focal adhesion; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.590; -; 1.
DR InterPro; IPR027450; AlkB-like.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR032862; ALKBH6.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR PANTHER; PTHR46030; PTHR46030; 1.
DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Dioxygenase; Iron; Metal-binding; Nucleus; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..238
FT /note="Alpha-ketoglutarate-dependent dioxygenase alkB
FT homolog 6"
FT /id="PRO_0000323717"
FT DOMAIN 96..227
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 103..105
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000250"
FT BINDING 114
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 116
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 182
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 218..224
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000250"
SQ SEQUENCE 238 AA; 26776 MW; E67EA27846A9121D CRC64;
MEEQDARVPA LEPFRVEQAP PLIYYVPDFI SKEEEEYLLR QVFNAPKPKW TQLSGRKLQN
WGGLPHPRGM VPERLPPWLQ RYVDKVSDLS LFGGLPANHV LVNQYLPGEG IMPHEDGPLY
YPTVSTISLG SHTVLDFYEP RQPDDDVPME QPRPPQRPIT SLLVEPRSLL VLRGTAYTRL
LHGISATRVD ELDATSLPPN ATACKSALPG AHLVRGTRVS LTIRRVPRVL RASLLLSK
