ALKBH_ARATH
ID ALKBH_ARATH Reviewed; 345 AA.
AC Q9SA98; A2RVV6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Alpha-ketoglutarate-dependent dioxygenase alkB;
DE EC=1.14.11.-;
DE AltName: Full=Alkylated DNA repair protein alkB homolog;
GN OrderedLocusNames=At1g11780; ORFNames=F25C20.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Bautista V.R., Chen H., De Los Reyes C., Wu S.Y., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Putative dioxygenase that may repair alkylated DNA or RNA by
CC oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate
CC and iron (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE-
CC ProRule:PRU00805};
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD30244.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007296; AAD30244.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28784.1; -; Genomic_DNA.
DR EMBL; BT030097; ABN04835.1; -; mRNA.
DR PIR; G86251; G86251.
DR RefSeq; NP_172643.1; NM_101050.4.
DR AlphaFoldDB; Q9SA98; -.
DR SMR; Q9SA98; -.
DR STRING; 3702.AT1G11780.1; -.
DR PaxDb; Q9SA98; -.
DR PRIDE; Q9SA98; -.
DR ProteomicsDB; 245024; -.
DR DNASU; 837723; -.
DR EnsemblPlants; AT1G11780.1; AT1G11780.1; AT1G11780.
DR GeneID; 837723; -.
DR Gramene; AT1G11780.1; AT1G11780.1; AT1G11780.
DR KEGG; ath:AT1G11780; -.
DR Araport; AT1G11780; -.
DR TAIR; locus:2027347; AT1G11780.
DR eggNOG; KOG2731; Eukaryota.
DR HOGENOM; CLU_029471_2_0_1; -.
DR InParanoid; Q9SA98; -.
DR OMA; WDTKKYS; -.
DR OrthoDB; 1045321at2759; -.
DR PhylomeDB; Q9SA98; -.
DR PRO; PR:Q9SA98; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SA98; baseline and differential.
DR Genevisible; Q9SA98; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0008198; F:ferrous iron binding; IBA:GO_Central.
DR GO; GO:0035516; F:oxidative DNA demethylase activity; IBA:GO_Central.
DR GO; GO:0035515; F:oxidative RNA demethylase activity; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0035513; P:oxidative RNA demethylation; IBA:GO_Central.
DR GO; GO:0035552; P:oxidative single-stranded DNA demethylation; IBA:GO_Central.
DR Gene3D; 2.60.120.590; -; 1.
DR InterPro; IPR004574; Alkb.
DR InterPro; IPR027450; AlkB-like.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR PANTHER; PTHR16557; PTHR16557; 1.
DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 2: Evidence at transcript level;
KW Dioxygenase; DNA damage; DNA repair; Iron; Metal-binding; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..345
FT /note="Alpha-ketoglutarate-dependent dioxygenase alkB"
FT /id="PRO_0000066670"
FT DOMAIN 225..345
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 179
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 186..188
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 232..234
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000250"
FT BINDING 243
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 245
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 299
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
FT BINDING 336..342
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000250"
FT BINDING 336
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805"
SQ SEQUENCE 345 AA; 39020 MW; 42882F3B7EA9376D CRC64;
MYESANVSDD ADRTAFRRAE KKYKLYYEQD SKFSRKKKLP KPIDLSELLD FNLISQNFNN
DGVLPDGIRV SKVDSSPVFC IDNRPGFYFI PDALSLKEQC KWIKESLTSF PQPPNRTNHN
AIYGPIDDLF DSAKENKVLV QDDLTNNKWK FYEEVDIEKA TRSSCKSVSA SVLLRKLRWS
TLGLQFDWSK RNYDVSLPHN NIPDALCQLA KTHAAIAMPD GEEFRPEGAI VNYFGIGDTL
GGHLDDMEAD WSKPIVSMSL GCKAIFLLGG KSKDDPPHAM YLRSGDVVLM AGEARECFHG
IPRIFTGEEN ADIGALESEL SHESGHFFAE YIKTSRININ IRQVF
