ALKB_DICDI
ID ALKB_DICDI Reviewed; 393 AA.
AC Q54N08;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Alpha-ketoglutarate-dependent dioxygenase alkB;
DE EC=1.14.11.-;
DE AltName: Full=Alkylated DNA repair protein alkB;
GN Name=alkB; ORFNames=DDB_G0285575;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA Skelton J., Ivens A., Bozzaro S.;
RT "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT bacteria in Dictyostelium.";
RL BMC Genomics 9:291-291(2008).
CC -!- FUNCTION: Putative dioxygenase that may repair alkylated DNA or RNA by
CC oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate
CC and iron (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Note=Binds 1 Fe(2+) ion per subunit.;
CC -!- INDUCTION: Up-regulated by phagocytic stimuli.
CC {ECO:0000269|PubMed:18559084}.
CC -!- SIMILARITY: Belongs to the alkB family. {ECO:0000305}.
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DR EMBL; AAFI02000079; EAL64625.1; -; Genomic_DNA.
DR RefSeq; XP_638135.1; XM_633043.1.
DR AlphaFoldDB; Q54N08; -.
DR SMR; Q54N08; -.
DR STRING; 44689.DDB0234218; -.
DR PaxDb; Q54N08; -.
DR EnsemblProtists; EAL64625; EAL64625; DDB_G0285575.
DR GeneID; 8625183; -.
DR KEGG; ddi:DDB_G0285575; -.
DR dictyBase; DDB_G0285575; alkB.
DR eggNOG; KOG2731; Eukaryota.
DR HOGENOM; CLU_029471_1_0_1; -.
DR InParanoid; Q54N08; -.
DR OMA; LDDCPAY; -.
DR PhylomeDB; Q54N08; -.
DR PRO; PR:Q54N08; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0008198; F:ferrous iron binding; IBA:GO_Central.
DR GO; GO:0035516; F:oxidative DNA demethylase activity; IBA:GO_Central.
DR GO; GO:0035515; F:oxidative RNA demethylase activity; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0035513; P:oxidative RNA demethylation; IBA:GO_Central.
DR GO; GO:0035552; P:oxidative single-stranded DNA demethylation; IBA:GO_Central.
DR Gene3D; 2.60.120.590; -; 1.
DR InterPro; IPR004574; Alkb.
DR InterPro; IPR027450; AlkB-like.
DR InterPro; IPR037151; AlkB-like_sf.
DR PANTHER; PTHR16557; PTHR16557; 1.
DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1.
PE 2: Evidence at transcript level;
KW Dioxygenase; DNA damage; DNA repair; Iron; Metal-binding; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..393
FT /note="Alpha-ketoglutarate-dependent dioxygenase alkB"
FT /id="PRO_0000384446"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 181..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..197
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 217
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 224..226
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 267..269
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000250"
FT BINDING 278
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 280
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 308
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 334
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 370..376
FT /ligand="2-oxoglutarate"
FT /ligand_id="ChEBI:CHEBI:16810"
FT /evidence="ECO:0000250"
SQ SEQUENCE 393 AA; 45446 MW; E0D46954120D840B CRC64;
MYETEEDPTT SNVATTNNEK TASTAATTTE PVKKVTEFMR VQRLFRQVTK SANGKNIPKE
KREPIDYSPV LDFHNLENNT EENKKLIIDC TSNVTTHDFE FNRDTEFYLH PREWKVYGLQ
GYPGFYFIKS PFTASQQKKW IKHALEDYAD PPNNNNITLF HGPIKNLWKN GEKELINEEL
KSQGKHDDDE IEQPTRPLDK NGEPLPTYRQ LLDKLAWSTL GYQYQWTPRL YSEEFYEEFP
DDLQELVQKI AIATKFDPYV AEAATVNFYS EDSIMGGHLD DAEQEMEKPI ISISFGSTAV
FLMGAETRDI APVPLFIRSG DIVIMGGRSR YCYHGVAKIV ENSFDLGLID ENDDQDLKYK
IQWLKEKNRR VNINTRQVFK VPKLGGKLIN KNE
