ID   ALKD_PSEAE              Reviewed;         220 AA.
AC   O68283; Q7DCB2;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 2.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=2-dehydro-3-deoxy-phosphogluconate aldolase;
DE            EC=4.1.2.14;
DE   AltName: Full=KDPG-aldolase;
DE   AltName: Full=Phospho-2-dehydro-3-deoxygluconate aldolase;
DE   AltName: Full=Phospho-2-keto-3-deoxygluconate aldolase;
GN   Name=eda; OrderedLocusNames=PA3181;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RA   Hager P.W., Dail M.B., Phibbs P.V. Jr.;
RT   "HexR, zwf, and eda genes.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-dehydro-3-deoxy-6-phospho-D-gluconate = D-glyceraldehyde 3-
CC         phosphate + pyruvate; Xref=Rhea:RHEA:17089, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:57569, ChEBI:CHEBI:59776; EC=4.1.2.14;
CC   -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the KHG/KDPG aldolase family. {ECO:0000305}.
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DR   EMBL; AF029673; AAC38312.2; -; Genomic_DNA.
DR   EMBL; AE004091; AAG06569.1; -; Genomic_DNA.
DR   PIR; G83247; G83247.
DR   RefSeq; NP_251871.1; NC_002516.2.
DR   RefSeq; WP_003113440.1; NZ_QZGE01000023.1.
DR   AlphaFoldDB; O68283; -.
DR   SMR; O68283; -.
DR   STRING; 287.DR97_4755; -.
DR   PaxDb; O68283; -.
DR   PRIDE; O68283; -.
DR   DNASU; 882580; -.
DR   EnsemblBacteria; AAG06569; AAG06569; PA3181.
DR   GeneID; 882580; -.
DR   KEGG; pae:PA3181; -.
DR   PATRIC; fig|208964.12.peg.3325; -.
DR   PseudoCAP; PA3181; -.
DR   HOGENOM; CLU_077795_1_1_6; -.
DR   InParanoid; O68283; -.
DR   OMA; GDWARIQ; -.
DR   PhylomeDB; O68283; -.
DR   BioCyc; PAER208964:G1FZ6-3241-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0008675; F:2-dehydro-3-deoxy-phosphogluconate aldolase activity; IEA:UniProtKB-EC.
DR   CDD; cd00452; KDPG_aldolase; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR000887; Aldlse_KDPG_KHG.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR031337; KDPG/KHG_AS_1.
DR   InterPro; IPR031338; KDPG/KHG_AS_2.
DR   PANTHER; PTHR30246; PTHR30246; 1.
DR   Pfam; PF01081; Aldolase; 1.
DR   TIGRFAMs; TIGR01182; eda; 1.
DR   PROSITE; PS00159; ALDOLASE_KDPG_KHG_1; 1.
DR   PROSITE; PS00160; ALDOLASE_KDPG_KHG_2; 1.
PE   3: Inferred from homology;
KW   Lyase; Reference proteome; Schiff base.
FT   CHAIN           1..220
FT                   /note="2-dehydro-3-deoxy-phosphogluconate aldolase"
FT                   /id="PRO_0000287743"
FT   ACT_SITE        52
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10118"
FT   ACT_SITE        136
FT                   /note="Schiff-base intermediate with substrate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10118"
SQ   SEQUENCE   220 AA;  23954 MW;  E130639230FBA58F CRC64;
     MHNLEQKTAR IDTLCREARI LPVITIDREA DILPMADALA AGGLTALEIT LRTAHGLTAI
     RRLSEERPHL RIGAGTVLDP RTFAAAEKAG ASFVVTPGCT DELLRFALDS EVPLLPGVAS
     ASEIMLAYRH GYRRFKLFPA EVSGGPAALK AFSGPFPDIR FCPTGGVSLN NLADYLAVPN
     VMCVGGTWML PKAVVDRGDW AQVERLSREA LERFAEHRRH