ID   ALKJ_PSEOL              Reviewed;         558 AA.
AC   Q00593;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Alcohol dehydrogenase [acceptor];
DE            EC=1.1.99.-;
GN   Name=alkJ; Synonyms=alcO;
OS   Pseudomonas oleovorans.
OG   Plasmid OCT.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas;
OC   Pseudomonas oleovorans/pseudoalcaligenes group.
OX   NCBI_TaxID=301;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-10.
RC   STRAIN=GPo1;
RX   PubMed=1453953; DOI=10.1111/j.1365-2958.1992.tb01769.x;
RA   van Beilen J.B., Eggink G., Enequist H., Bos R., Witholt B.;
RT   "DNA sequence determination and functional characterization of the OCT-
RT   plasmid-encoded alkJKL genes of Pseudomonas oleovorans.";
RL   Mol. Microbiol. 6:3121-3136(1992).
CC   -!- FUNCTION: Converts aliphatic medium-chain-length alcohols into
CC       aldehydes. May be linked to the electron transfer chain.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=A + a primary alcohol = AH2 + an aldehyde;
CC         Xref=Rhea:RHEA:14685, ChEBI:CHEBI:13193, ChEBI:CHEBI:15734,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:17499;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane.
CC   -!- SIMILARITY: Belongs to the GMC oxidoreductase family. {ECO:0000305}.
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DR   EMBL; AJ245436; CAB54054.1; -; Genomic_DNA.
DR   PIR; S27994; S27994.
DR   AlphaFoldDB; Q00593; -.
DR   SMR; Q00593; -.
DR   BioCyc; MetaCyc:MON-1081; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0047645; F:alkan-1-ol dehydrogenase (acceptor) activity; IEA:RHEA.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR012132; GMC_OxRdtase.
DR   InterPro; IPR000172; GMC_OxRdtase_N.
DR   InterPro; IPR007867; GMC_OxRtase_C.
DR   PANTHER; PTHR11552; PTHR11552; 1.
DR   Pfam; PF05199; GMC_oxred_C; 1.
DR   Pfam; PF00732; GMC_oxred_N; 1.
DR   PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   PROSITE; PS00623; GMC_OXRED_1; 1.
DR   PROSITE; PS00624; GMC_OXRED_2; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Direct protein sequencing; FAD;
KW   Flavoprotein; Membrane; Oxidoreductase; Plasmid.
FT   CHAIN           1..558
FT                   /note="Alcohol dehydrogenase [acceptor]"
FT                   /id="PRO_0000205578"
FT   ACT_SITE        469
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:E4QP00"
FT   BINDING         3..32
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   558 AA;  61195 MW;  DF4363ECC71B02E8 CRC64;
     MYDYIIVGAG SAGCVLANRL SADPSKRVCL LEAGPRDTNP LIHMPLGIAL LSNSKKLNWA
     FQTAPQQNLN GRSLFWPRGK TLGGSSSINA MVYIRGHEDD YHAWEQAAGR YWGWYRALEL
     FKRLECNQRF DKSEHHGVDG ELAVSDLKYI NPLSKAFVQA GMEANINFNG DFNGEYQDGV
     GFYQVTQKNG QRWSSARAFL HGVLSRPNLD IITDAHASKI LFEDRKAVGV SYIKKNMHHQ
     VKTTSGGEVL LSLGAVGTPH LLMLSGVGAA AELKEHGVSL VHDLPEVGKN LQDHLDITLM
     CAANSREPIG VALSFIPRGV SGLFSYVFKR EGFLTSNVAE SGGFVKSSPD RDRPNLQFHF
     LPTYLKDHGR KIAGGYGYTL HICDLLPKSR GRIGLKSANP LQPPLIDPNY LSDHEDIKTM
     IAGIKIGRAI LQAPSMAKHF KHEVVPGQAV KTDDEIIEDI RRRAETIYHP VGTCRMGKDP
     ASVVDPCLKI RGLANIRVVD ASIMPHLVAG NTNAPTIMIA ENAAEIIMRN LDVEALEASA
     EFAREGAELE LAMIAVCM