ID   ALKK_PSEOL              Reviewed;         546 AA.
AC   Q00594;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Medium-chain-fatty-acid--CoA ligase;
DE            EC=6.2.1.2 {ECO:0000269|PubMed:1453953};
DE   AltName: Full=Medium-chain acyl-CoA synthetase;
GN   Name=alkK {ECO:0000303|PubMed:1453953};
OS   Pseudomonas oleovorans.
OG   Plasmid OCT.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas;
OC   Pseudomonas oleovorans/pseudoalcaligenes group.
OX   NCBI_TaxID=301;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, PATHWAY, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=GPo1;
RX   PubMed=1453953; DOI=10.1111/j.1365-2958.1992.tb01769.x;
RA   van Beilen J.B., Eggink G., Enequist H., Bos R., Witholt B.;
RT   "DNA sequence determination and functional characterization of the OCT-
RT   plasmid-encoded alkJKL genes of Pseudomonas oleovorans.";
RL   Mol. Microbiol. 6:3121-3136(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a medium chain fatty acid + ATP + CoA = a medium-chain fatty
CC         acyl-CoA + AMP + diphosphate; Xref=Rhea:RHEA:48340,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:59558, ChEBI:CHEBI:90546, ChEBI:CHEBI:456215; EC=6.2.1.2;
CC         Evidence={ECO:0000269|PubMed:1453953};
CC   -!- PATHWAY: Lipid metabolism; fatty acid metabolism.
CC       {ECO:0000269|PubMed:1453953}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:1453953}.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AJ245436; CAB54055.1; -; Genomic_DNA.
DR   PIR; S27995; S27995.
DR   AlphaFoldDB; Q00594; -.
DR   SMR; Q00594; -.
DR   BioCyc; MetaCyc:MON-1083; -.
DR   UniPathway; UPA00199; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0031956; F:medium-chain fatty acid-CoA ligase activity; IEA:RHEA.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; -; 1.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   InterPro; IPR042099; ANL_N_sf.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cytoplasm; Fatty acid metabolism; Ligase; Lipid metabolism;
KW   Magnesium; Metal-binding; Nucleotide-binding; Plasmid.
FT   CHAIN           1..546
FT                   /note="Medium-chain-fatty-acid--CoA ligase"
FT                   /id="PRO_0000193142"
FT   BINDING         185
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         235
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         329
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         330
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         417
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         434
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         438
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         443
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   546 AA;  59262 MW;  DD7DA4A538010776 CRC64;
     MLGQMMRNQL VIGSLVEHAA RYHGAREVVS VETSGEVTRS CWKEVELRAR KLASALGKMG
     LTPSDRCATI AWNNIRHLEV YYAVSGAGMV CHTINPRLFI EQITYVINHA EDKVVLLDDT
     FLPIIAEIHG SLPKVKAFVL MAHNNSNASA QMPGLIAYED LIGQGDDNYI WPDVDENEAS
     SLCYTSGTTG NPKGVLYSHR STVLHSMTTA MPDTLNLSAR DTILPVVPMF HVNAWGTPYS
     AAMVGAKLVL PGPALDGASL SKLIASEGVS IALGVPVVWQ GLLAAQAGNG SKSQSLTRVV
     VGGSACPASM IREFNDIYGV EVIHAWGMTE LSPFGTANTP LAHHVDLSPD EKLSLRKSQG
     RPPYGVELKI VNDEGIRLPE DGRSKGNLMA RGHWVIKDYF HSDPGSTLSD GWFSTGDVAT
     IDSDGFMTIC DRAKDIIKSG GEWISTVELE SIAIAHPHIV DAAVIAARHE KWDERPLLIA
     VKSPNSELTS GEVCNYFADK VARWQIPDAA IFVEELPRNG TGKILKNRLR EKYGDILLRS
     SSSVCE