ID   ALKMO_DANRE             Reviewed;         446 AA.
AC   Q6NYE4;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Alkylglycerol monooxygenase;
DE            EC=1.14.16.5;
DE   AltName: Full=Transmembrane protein 195;
GN   Name=agmo; Synonyms=tmem195; ORFNames=zgc:77121;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Glyceryl-ether monooxygenase that cleaves the O-alkyl bond of
CC       ether lipids. Ether lipids are essential components of brain membranes
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + 1-O-(1,2-
CC         saturated-alkyl)-sn-glycerol + O2 = (6R)-L-erythro-6,7-
CC         dihydrobiopterin + a 1-(1-hydroxyalkyl)-sn-glycerol + H2O;
CC         Xref=Rhea:RHEA:36255, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:43120, ChEBI:CHEBI:59560, ChEBI:CHEBI:73418,
CC         ChEBI:CHEBI:83957; EC=1.14.16.5;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family. TMEM195 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC066626; AAH66626.1; -; mRNA.
DR   AlphaFoldDB; Q6NYE4; -.
DR   SMR; Q6NYE4; -.
DR   STRING; 7955.ENSDARP00000122798; -.
DR   PaxDb; Q6NYE4; -.
DR   ZFIN; ZDB-GENE-040426-2207; agmo.
DR   eggNOG; KOG0872; Eukaryota.
DR   InParanoid; Q6NYE4; -.
DR   PhylomeDB; Q6NYE4; -.
DR   Reactome; R-DRE-75109; Triglyceride biosynthesis.
DR   PRO; PR:Q6NYE4; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0050479; F:glyceryl-ether monooxygenase activity; ISS:UniProtKB.
DR   GO; GO:0005506; F:iron ion binding; ISS:UniProtKB.
DR   GO; GO:0046485; P:ether lipid metabolic process; ISS:UniProtKB.
DR   GO; GO:0008610; P:lipid biosynthetic process; IEA:InterPro.
DR   GO; GO:0006643; P:membrane lipid metabolic process; ISS:UniProtKB.
DR   InterPro; IPR006694; Fatty_acid_hydroxylase.
DR   Pfam; PF04116; FA_hydroxylase; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Iron; Lipid metabolism; Membrane; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..446
FT                   /note="Alkylglycerol monooxygenase"
FT                   /id="PRO_0000299303"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        167..187
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        329..349
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        362..382
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        410..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          117..248
FT                   /note="Fatty acid hydroxylase"
FT                   /evidence="ECO:0000255"
FT   MOTIF           131..135
FT                   /note="Histidine box-1"
FT   MOTIF           144..148
FT                   /note="Histidine box-2"
FT   MOTIF           220..224
FT                   /note="Histidine box-3"
SQ   SEQUENCE   446 AA;  51797 MW;  B8BD9A15CD55276D CRC64;
     MAQVEANNVT VSQGFRMLFS LMTPEESSFA TVEEVPKYVN QATPYFIGLI LLEIVLGWLK
     TDGPHIKIND FITSLSAGMM SRLPQLMMRS VELSAYIYIW DNFHFLELPW DSAWTWWLAF
     LGVDMGYYWF HRFAHELNIL WAGHQVHHSS EYYNLSTALR QSVTQQFSSW IFYSPLALLI
     PPSVFAVHIQ FNLLYQFWIH TELVKDLGPL ELILNTPSHH RVHHGRNPYC IDKNYAGILI
     IWDRMFGTFA PESDKVIYGL THPIGTFEIW SVEFLYYPYL WQRFWEVEGI SNKLSVIWKG
     PGWTPGKPRL GDIADIPQIT GEEKPHDPAW SPVMQAYVIL QFFLLLDVYN SLLLDQMILS
     ELTVILLTAY VLLSLTSLGF LIDQRSNAAE LEMLRCVLIM LLHRFGYIKP LLPALAFPME
     AFILISTIYW SLQSVKQRTD KMKKQN