ID   ALKMO_XENTR             Reviewed;         446 AA.
AC   Q5M8F9;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Alkylglycerol monooxygenase;
DE            EC=1.14.16.5;
DE   AltName: Full=Transmembrane protein 195;
GN   Name=agmo; Synonyms=tmem195;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Glyceryl-ether monooxygenase that cleaves the O-alkyl bond of
CC       ether lipids. Ether lipids are essential components of brain membranes
CC       (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + 1-O-(1,2-
CC         saturated-alkyl)-sn-glycerol + O2 = (6R)-L-erythro-6,7-
CC         dihydrobiopterin + a 1-(1-hydroxyalkyl)-sn-glycerol + H2O;
CC         Xref=Rhea:RHEA:36255, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:43120, ChEBI:CHEBI:59560, ChEBI:CHEBI:73418,
CC         ChEBI:CHEBI:83957; EC=1.14.16.5;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family. TMEM195 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC088041; AAH88041.1; -; mRNA.
DR   RefSeq; NP_001011313.1; NM_001011313.1.
DR   AlphaFoldDB; Q5M8F9; -.
DR   PRIDE; Q5M8F9; -.
DR   DNASU; 496770; -.
DR   GeneID; 496770; -.
DR   KEGG; xtr:496770; -.
DR   CTD; 392636; -.
DR   Xenbase; XB-GENE-950748; agmo.
DR   eggNOG; KOG0872; Eukaryota.
DR   InParanoid; Q5M8F9; -.
DR   OrthoDB; 1446475at2759; -.
DR   Reactome; R-XTR-75109; Triglyceride biosynthesis.
DR   Proteomes; UP000008143; Chromosome 6.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0050479; F:glyceryl-ether monooxygenase activity; ISS:UniProtKB.
DR   GO; GO:0005506; F:iron ion binding; ISS:UniProtKB.
DR   GO; GO:0046485; P:ether lipid metabolic process; ISS:UniProtKB.
DR   GO; GO:0008610; P:lipid biosynthetic process; IEA:InterPro.
DR   GO; GO:0006643; P:membrane lipid metabolic process; ISS:UniProtKB.
DR   InterPro; IPR006694; Fatty_acid_hydroxylase.
DR   Pfam; PF04116; FA_hydroxylase; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Iron; Lipid metabolism; Membrane; Oxidoreductase;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..446
FT                   /note="Alkylglycerol monooxygenase"
FT                   /id="PRO_0000299304"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        110..130
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        167..187
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        339..359
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        362..382
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        412..434
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          118..248
FT                   /note="Fatty acid hydroxylase"
FT                   /evidence="ECO:0000255"
FT   MOTIF           131..135
FT                   /note="Histidine box-1"
FT   MOTIF           144..148
FT                   /note="Histidine box-2"
FT   MOTIF           220..224
FT                   /note="Histidine box-3"
SQ   SEQUENCE   446 AA;  52027 MW;  2E2A271704EE1383 CRC64;
     MGGFTGQSEV SVSQGIRMMF YVLYPNETSF KNVEDVPDYV EKATVYFFIM LILEMIISWA
     WKGQPLRIND GLTSLSAGVF SRLPDVAFRG VEVASYIYIW NNYRLIELPW DSPWTWWLTF
     LGVDFGYYWF HRMAHEVNIM WAGHQTHHSS EDYNLTTALR QSFIQKYFSW MFYWPMAFCI
     PPSVFAVHIQ FNLLYQFWIH TELINNLGPL EWILNTPSHH RVHHGRNPYC IDSNYAGTLI
     IWDRMFGTFV PEKEKVLYGL THPINTFEPF QVQIQHCVLI WNTFWATPGF SNKLSVIFKG
     PGWGPGKPRL GLPEEIPQIT GEEKPYDPKS AGYLQCYGMM HFLLLLAVYV HMFEAKLILT
     PATLLLRIGY ILLTLTSLGF IFDKRPKAAL LETIRCLLFL FLQKYDYMTT DVPYLRIINE
     VTFSICTAAW GLKAVKEAIE RTKKLE