ALKR5_ARATH
ID ALKR5_ARATH Reviewed; 345 AA.
AC Q9ASZ9; F4HPY5; F4HPY6; O22710;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Probable aldo-keto reductase 5;
DE EC=1.1.1.-;
GN OrderedLocusNames=At1g60730; ORFNames=F8A5.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9ASZ9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ASZ9-2; Sequence=VSP_042348;
CC Name=3;
CC IsoId=Q9ASZ9-3; Sequence=VSP_042349, VSP_042350;
CC -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB71982.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002292; AAB71982.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33724.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33726.1; -; Genomic_DNA.
DR EMBL; AF361576; AAK32744.1; -; mRNA.
DR EMBL; AY124862; AAM70571.1; -; mRNA.
DR PIR; F96632; F96632.
DR RefSeq; NP_001185274.1; NM_001198345.1. [Q9ASZ9-2]
DR RefSeq; NP_564762.2; NM_104756.4. [Q9ASZ9-1]
DR AlphaFoldDB; Q9ASZ9; -.
DR SMR; Q9ASZ9; -.
DR STRING; 3702.AT1G60730.3; -.
DR iPTMnet; Q9ASZ9; -.
DR PRIDE; Q9ASZ9; -.
DR ProteomicsDB; 245000; -. [Q9ASZ9-1]
DR EnsemblPlants; AT1G60730.1; AT1G60730.1; AT1G60730. [Q9ASZ9-1]
DR EnsemblPlants; AT1G60730.3; AT1G60730.3; AT1G60730. [Q9ASZ9-2]
DR GeneID; 842367; -.
DR Gramene; AT1G60730.1; AT1G60730.1; AT1G60730. [Q9ASZ9-1]
DR Gramene; AT1G60730.3; AT1G60730.3; AT1G60730. [Q9ASZ9-2]
DR KEGG; ath:AT1G60730; -.
DR Araport; AT1G60730; -.
DR TAIR; locus:2036515; AT1G60730.
DR eggNOG; KOG1575; Eukaryota.
DR HOGENOM; CLU_023205_2_1_1; -.
DR InParanoid; Q9ASZ9; -.
DR OMA; RRMLPKY; -.
DR OrthoDB; 1136766at2759; -.
DR PhylomeDB; Q9ASZ9; -.
DR BioCyc; ARA:AT1G60730-MON; -.
DR PRO; PR:Q9ASZ9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ASZ9; baseline and differential.
DR Genevisible; Q9ASZ9; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0004033; F:aldo-keto reductase (NADP) activity; IBA:GO_Central.
DR Gene3D; 3.20.20.100; -; 1.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR SUPFAM; SSF51430; SSF51430; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..345
FT /note="Probable aldo-keto reductase 5"
FT /id="PRO_0000415744"
FT ACT_SITE 63
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 130
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 209..219
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT VAR_SEQ 141
FT /note="T -> TLIHEEPLSGEMILSSPLEFF (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_042348"
FT VAR_SEQ 234..251
FT /note="TLPRFQQENLDHNKILFE -> ANSTKIPTGKLRPQQDSL (in isoform
FT 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_042349"
FT VAR_SEQ 252..345
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_042350"
SQ SEQUENCE 345 AA; 37857 MW; 6D2778848FFC4F59 CRC64;
MAEACGVRRI KLGSQGLEVS AQGLGCMGLS AFYGTPKPET EAIALIHHAI HSGVTFLDTS
DIYGPETNEL LLSKALKDGV REKVELATKY GIRYAEGKVE FKGDPAYVRA ACEASLMRVD
VACIDLYYQH RIDTRVPIEI TIGELKKLVE EGKIKYIGLS EASASTIRRA HAVHPITALQ
IEWSLWSRDV EEDIIPTCRE LGIGIVAYSP LGRGFFASGP KLVENLDNND VRKTLPRFQQ
ENLDHNKILF EKVSAMSEKK GCTPAQLALA WVHHQGDDVC PIPGTTKIEN LNQNIGALSV
KLTPEEMSEL ESLAQPGFVK GERSISILTT FKNSETPPLS SWKAA
