ID   3NO2N_NAJKA             Reviewed;          53 AA.
AC   P0DSM9;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   05-JUN-2019, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Weak toxin NWT {ECO:0000303|PubMed:19377518};
OS   Naja kaouthia (Monocled cobra) (Naja siamensis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8649;
RN   [1]
RP   PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, AND
RP   SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=19377518; DOI=10.1134/s1068162009010026;
RA   Starkov V.G., Polyak Y.L., Vulfius E.A., Kryukova E.V., Tsetlin V.I.,
RA   Utkin Y.N.;
RT   "New weak toxins from the cobra venom.";
RL   Russ. J. Bioorg. Chem. 35:15-24(2009).
CC   -!- FUNCTION: Binds with low affinity and weakly inhibits muscle nicotinic
CC       acetylcholine receptor (nAChR). {ECO:0000250|UniProtKB:O93422}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19377518}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:19377518}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Ancestral
CC       subfamily. Orphan group II sub-subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DSM9; -.
DR   SMR; P0DSM9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Toxin.
FT   CHAIN           1..53
FT                   /note="Weak toxin NWT"
FT                   /evidence="ECO:0000269|PubMed:19377518"
FT                   /id="PRO_0000447297"
FT   DISULFID        6..11
FT                   /evidence="ECO:0000250|UniProtKB:Q8AY51"
FT   DISULFID        17..33
FT                   /evidence="ECO:0000250|UniProtKB:Q8AY51"
FT   DISULFID        37..48
FT                   /evidence="ECO:0000250|UniProtKB:Q8AY51"
SQ   SEQUENCE   53 AA;  6139 MW;  6AA56FCFE1C678A6 CRC64;
     LTCLICPEKY CNKVHTCLNG EKIYDQRKYI RGCADTCPVR KPREIVECCS TDK