ALL21_DERFA
ID ALL21_DERFA Reviewed; 136 AA.
AC B2GM84;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 25-MAY-2022, entry version 31.
DE RecName: Full=Mite allergen Der f 21.0101 {ECO:0000305};
DE AltName: Full=Allergen Der f 21 {ECO:0000303|PubMed:27069539, ECO:0000312|EMBL:AHC94806.1};
DE AltName: Full=Mite group 21 allergen Der f 21 {ECO:0000303|PubMed:25628789, ECO:0000303|PubMed:26527267, ECO:0000303|PubMed:30894561};
DE AltName: Allergen=Der f 21.0101 {ECO:0000305};
DE Flags: Precursor;
OS Dermatophagoides farinae (American house dust mite).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Acariformes; Sarcoptiformes; Astigmata; Psoroptidia; Analgoidea;
OC Pyroglyphidae; Dermatophagoidinae; Dermatophagoides.
OX NCBI_TaxID=6954 {ECO:0000312|EMBL:AAX34048.1};
RN [1] {ECO:0000312|EMBL:AAX34048.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Chew F.T., Gao Y.F., Ong S.T.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=25628789;
RA Cui Y., Jiang Y., Ji Y., Zhou Y., Yu L., Wang N., Yang L., Zhang C.;
RT "Cloning, expression, and analysis of a cDNA coding for the
RT Dermatophagoides farinae group 21 (Der f 21) allergen.";
RL Am. J. Transl. Res. 6:786-792(2014).
RN [3]
RP TISSUE SPECIFICITY, AND ALLERGEN.
RX PubMed=27069539;
RA Wu Y., Jiang C., Li M., Yu H., Xiao X., Fan X., Lin J., Liu X., Zhang M.,
RA Yang P., Liu Z.;
RT "Der f 21, a novel allergen from dermatophagoides farina.";
RL Am. J. Transl. Res. 8:49-59(2016).
RN [4]
RP CRYSTALLIZATION, AND SUBUNIT.
RX PubMed=26527267; DOI=10.1107/s2053230x1501818x;
RA Pang S.L., Ho K.L., Waterman J., Teh A.H., Chew F.T., Ng C.L.;
RT "Cloning, expression, purification, characterization, crystallization and
RT X-ray crystallographic analysis of recombinant Der f 21 (rDer f 21) from
RT Dermatophagoides farinae.";
RL Acta Crystallogr. F Struct. Biol. Commun. 71:1396-1400(2015).
RN [5] {ECO:0007744|PDB:5YNX, ECO:0007744|PDB:5YNY}
RP X-RAY CRYSTALLOGRAPHY (1.49 ANGSTROMS) OF 25-136, SUBUNIT, ALLERGEN, AND
RP MUTAGENESIS OF GLU-25; ASP-26; LYS-27; HIS-34; GLU-38; GLU-39; GLU-41;
RP GLU-42; LYS-43; ASP-45; GLU-48; HIS-49; GLU-56; LYS-59; GLU-60; GLU-62;
RP LYS-63; LYS-65; LYS-67; GLU-68; GLU-71; ARG-75; GLU-76; GLU-81; ARG-85;
RP LYS-89; LYS-96; ASP-99; ARG-105; GLU-109; GLU-116; LYS-120; ASP-121;
RP GLU-124; LYS-127; LYS-128; LYS-130 AND LYS-133.
RX PubMed=30894561; DOI=10.1038/s41598-019-40879-x;
RA Pang S.L., Ho K.L., Waterman J., Rambo R.P., Teh A.H., Mathavan I.,
RA Harris G., Beis K., Say Y.H., Anusha M.S., Sio Y.Y., Chew F.T., Ng C.L.;
RT "Crystal structure and epitope analysis of house dust mite allergen Der f
RT 21.";
RL Sci. Rep. 9:4933-4933(2019).
CC -!- SUBUNIT: Monomer. Homodimer. {ECO:0000269|PubMed:26527267,
CC ECO:0000269|PubMed:30894561}.
CC -!- TISSUE SPECIFICITY: Highly expressed in foregut (stomach), midgut and
CC hindgut. Not expressed in body wall, reproductive system or body
CC cavity. {ECO:0000269|PubMed:27069539}.
CC -!- ALLERGEN: Causes an allergic reaction in human (PubMed:27069539,
CC PubMed:30894561). Binds to IgE in 29% of the 38 dust mite allergic
CC children tested. 43% of the 98 dust mite allergic adults tested show a
CC positive response to this protein by skin prick test (PubMed:27069539).
CC Binds to IgE in all of the 24 D.farinae allergic patients tested. There
CC is a positive correlation between the number of specific major epitope
CC residues in this allergen and the specific IgE level of the atopic
CC population. The higher the IgE level of the patient for this protein,
CC the higher the number of recognized epitope residues in this allergen
CC by the IgE of the individual out of the 38 determined surface-exposed
CC epitopes tested (PubMed:30894561). Causes an allergic reaction in mice.
CC Mice allergic to this protein display elevated specific IgE and IgG1,
CC increased levels of interferon gamma and interleukin-4 as well as
CC increased proliferation of the spleen cells, airway
CC hyperresponsiveness, large quantity of exudates in the airway lumen and
CC profound inflammatory cell infiltration in the lung tissue
CC (PubMed:27069539). {ECO:0000269|PubMed:27069539,
CC ECO:0000269|PubMed:30894561}.
CC -!- SIMILARITY: Belongs to the mite group 5 allergen family. {ECO:0000305}.
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DR EMBL; AY800349; AAX34048.1; -; mRNA.
DR EMBL; KF732965; AHC94806.1; -; mRNA.
DR PDB; 5YNX; X-ray; 1.49 A; A/B=25-135.
DR PDB; 5YNY; X-ray; 2.30 A; A/B/C/D=25-136.
DR PDBsum; 5YNX; -.
DR PDBsum; 5YNY; -.
DR AlphaFoldDB; B2GM84; -.
DR SMR; B2GM84; -.
DR Allergome; 11615; Der f 21.0101.
DR Allergome; 305; Der f 5.
DR Allergome; 5843; Der f 21.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR Gene3D; 1.20.58.970; -; 1.
DR InterPro; IPR020306; Mite_allergen_group-5/21.
DR InterPro; IPR038455; Mite_allergen_group-5/21_sf.
DR Pfam; PF11642; Blo-t-5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Coiled coil; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..136
FT /note="Mite allergen Der f 21.0101"
FT /evidence="ECO:0000255"
FT /id="PRO_5007639490"
FT MUTAGEN 25
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 26
FT /note="D->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 27
FT /note="K->A: Decreased IgE-binding by more than half of the
FT 24 patients tested. Identified as a major epitope."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 34
FT /note="H->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 38
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 39
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 41
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 42
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 43
FT /note="K->A: Decreased IgE-binding by more than half of the
FT 24 patients tested. Identified as a major epitope."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 45
FT /note="D->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 48
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 49
FT /note="H->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 56
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 59
FT /note="K->A: Decreased IgE-binding by more than half of the
FT 24 patients tested. Identified as a major epitope."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 60
FT /note="E->A: Decreased IgE-binding by more than half of the
FT 24 patients tested. Identified as a major epitope."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 62
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 63
FT /note="K->A: Decreased IgE-binding by more than half of the
FT 24 patients tested. Identified as a major epitope."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 65
FT /note="K->A: SDecreased IgE-binding by more than half of
FT the 24 patients tested. Identified as a major epitope."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 67
FT /note="K->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 68
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 71
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 75
FT /note="R->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 76
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 81
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 85
FT /note="R->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 89
FT /note="K->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 96
FT /note="K->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 99
FT /note="D->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 105
FT /note="R->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 109
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 116
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 120
FT /note="K->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 121
FT /note="D->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 124
FT /note="E->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 127
FT /note="K->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 128
FT /note="K->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 130
FT /note="K->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT MUTAGEN 133
FT /note="K->A: Decreaced IgE-binding by some of the 24
FT patients tested."
FT /evidence="ECO:0000269|PubMed:30894561"
FT STRAND 25..27
FT /evidence="ECO:0007829|PDB:5YNY"
FT HELIX 31..64
FT /evidence="ECO:0007829|PDB:5YNX"
FT HELIX 67..95
FT /evidence="ECO:0007829|PDB:5YNX"
FT HELIX 102..130
FT /evidence="ECO:0007829|PDB:5YNX"
SQ SEQUENCE 136 AA; 15965 MW; 278871A9F1E8ACBE CRC64;
MKFIIFCAIV MAVSVSGFIV DVDTEDKWRN AFDHMLMEEF EEKMDQIEHG LLMLSEQYKE
LEKTKSKELK EQILRELTIA ENYLRGALKF MQQEAKRTDL NMFERYNFET AVSTIEILVK
DLAELAKKVK AVKSDD
