ALL2_AEDAE
ID ALL2_AEDAE Reviewed; 321 AA.
AC P18153; O96364; Q0IF92;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 2.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=37 kDa salivary gland allergen Aed a 2;
DE AltName: Full=Protein D7;
DE AltName: Allergen=Aed a 2;
DE Flags: Precursor;
GN Name=D7; ORFNames=AAEL006424;
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=Rockefeller; TISSUE=Salivary gland;
RX PubMed=2052024; DOI=10.1016/0166-6851(91)90010-4;
RA James A.A., Blackmer K., Marinotti O., Ghosn C.R., Racioppi J.V.;
RT "Isolation and characterization of the gene expressing the major salivary
RT gland protein of the female mosquito, Aedes aegypti.";
RL Mol. Biochem. Parasitol. 44:245-254(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Liverpool;
RX PubMed=17519023; DOI=10.1186/gb-2007-8-5-r88;
RA Lobo N.F., Campbell K.S., Thaner D., Debruyn B., Koo H., Gelbart W.M.,
RA Loftus B.J., Severson D.W., Collins F.H.;
RT "Analysis of 14 BAC sequences from the Aedes aegypti genome: a benchmark
RT for genome annotation and assembly.";
RL Genome Biol. 8:R88.1-R88.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVPib12;
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 16-286 (ISOFORM B).
RX PubMed=10436938; DOI=10.1016/s0965-1748(99)00042-9;
RA Brown S.E., Wood S.H., Knudson D.L.;
RT "Vertebrate exon trapping methods: implications for transcript mapping with
RT mosquito DNA.";
RL Insect Biochem. Mol. Biol. 29:643-651(1999).
CC -!- FUNCTION: Thought to be involved in blood-feeding.
CC {ECO:0000269|PubMed:2052024}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2052024}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=P18153-1; Sequence=Displayed;
CC Name=B;
CC IsoId=P18153-2; Sequence=VSP_034863, VSP_034864, VSP_034865;
CC -!- TISSUE SPECIFICITY: Expressed in the distal-lateral and medial lobes of
CC the adult female salivary gland. {ECO:0000269|PubMed:2052024}.
CC -!- ALLERGEN: Causes an allergic reaction in human.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M33156; AAA29347.1; -; Genomic_DNA.
DR EMBL; M33157; AAA29348.1; -; mRNA.
DR EMBL; EF173376; ABM68616.1; -; Genomic_DNA.
DR EMBL; CH477389; EAT41994.1; -; Genomic_DNA.
DR EMBL; AF027814; AAD01849.1; -; mRNA.
DR RefSeq; XP_001657779.1; XM_001657729.1. [P18153-1]
DR PDB; 3DXL; X-ray; 1.30 A; A=20-321.
DR PDB; 3DY9; X-ray; 1.70 A; A=20-321.
DR PDB; 3DYE; X-ray; 1.75 A; A=20-321.
DR PDB; 3DZT; X-ray; 1.80 A; A=20-321.
DR PDBsum; 3DXL; -.
DR PDBsum; 3DY9; -.
DR PDBsum; 3DYE; -.
DR PDBsum; 3DZT; -.
DR AlphaFoldDB; P18153; -.
DR SMR; P18153; -.
DR STRING; 7159.AAEL006424-PA; -.
DR Allergome; 3540; Aed a 2.0101.
DR Allergome; 4; Aed a 2.
DR GeneID; 5567958; -.
DR KEGG; aag:5567958; -.
DR VEuPathDB; VectorBase:AAEL006424; -.
DR VEuPathDB; VectorBase:AAEL026087; -.
DR eggNOG; ENOG502T82F; Eukaryota.
DR HOGENOM; CLU_077766_0_0_1; -.
DR InParanoid; P18153; -.
DR OMA; TRCMEDN; -.
DR OrthoDB; 1039968at2759; -.
DR PhylomeDB; P18153; -.
DR EvolutionaryTrace; P18153; -.
DR Proteomes; UP000008820; Chromosome 2.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005549; F:odorant binding; IEA:InterPro.
DR Gene3D; 1.10.238.20; -; 2.
DR InterPro; IPR006170; PBP/GOBP.
DR InterPro; IPR036728; PBP_GOBP_sf.
DR Pfam; PF01395; PBP_GOBP; 2.
DR SMART; SM00708; PhBP; 2.
DR SUPFAM; SSF47565; SSF47565; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Alternative splicing; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..321
FT /note="37 kDa salivary gland allergen Aed a 2"
FT /id="PRO_0000021059"
FT VAR_SEQ 74..87
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10436938"
FT /id="VSP_034863"
FT VAR_SEQ 161
FT /note="G -> V (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10436938"
FT /id="VSP_034864"
FT VAR_SEQ 162..226
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10436938"
FT /id="VSP_034865"
FT CONFLICT 4
FT /note="P -> L (in Ref. 1; AAA29347)"
FT /evidence="ECO:0000305"
FT CONFLICT 10
FT /note="V -> F (in Ref. 1; AAA29347)"
FT /evidence="ECO:0000305"
FT CONFLICT 19
FT /note="T -> M (in Ref. 1; AAA29347 and 4; AAD01849)"
FT /evidence="ECO:0000305"
FT CONFLICT 30
FT /note="T -> I (in Ref. 1; AAA29347 and 4; AAD01849)"
FT /evidence="ECO:0000305"
FT CONFLICT 43
FT /note="P -> A (in Ref. 1; AAA29347 and 4; AAD01849)"
FT /evidence="ECO:0000305"
FT CONFLICT 115
FT /note="Q -> K (in Ref. 1; AAA29347 and 4; AAD01849)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="Q -> K (in Ref. 1; AAA29348)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="K -> Q (in Ref. 1; AAA29347)"
FT /evidence="ECO:0000305"
FT CONFLICT 221..226
FT /note="NNELDA -> DNQLDV (in Ref. 1; AAA29347)"
FT /evidence="ECO:0000305"
FT CONFLICT 234..235
FT /note="MQ -> KL (in Ref. 1; AAA29347/AAA29348 and 4;
FT AAD01849)"
FT /evidence="ECO:0000305"
FT CONFLICT 259
FT /note="G -> K (in Ref. 1; AAA29347/AAA29348 and 4;
FT AAD01849)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="V -> A (in Ref. 1; AAA29347/AAA29348)"
FT /evidence="ECO:0000305"
FT HELIX 24..38
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 45..52
FT /evidence="ECO:0007829|PDB:3DXL"
FT TURN 53..55
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 63..75
FT /evidence="ECO:0007829|PDB:3DXL"
FT STRAND 78..80
FT /evidence="ECO:0007829|PDB:3DXL"
FT TURN 81..84
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 89..97
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 99..101
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 104..115
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 124..137
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 139..145
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 150..160
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 161..163
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 171..179
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 187..189
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 190..193
FT /evidence="ECO:0007829|PDB:3DXL"
FT TURN 194..196
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 202..214
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 226..235
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 241..252
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 258..260
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 261..271
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 275..289
FT /evidence="ECO:0007829|PDB:3DXL"
FT TURN 292..295
FT /evidence="ECO:0007829|PDB:3DXL"
FT STRAND 296..298
FT /evidence="ECO:0007829|PDB:3DXL"
FT HELIX 304..318
FT /evidence="ECO:0007829|PDB:3DYE"
SQ SEQUENCE 321 AA; 36895 MW; 9BAF0F0335B93070 CRC64;
MKLPLLLAIV TTFSVVASTG PFDPEEMLFT FTRCMEDNLE DGPNRLPMLA KWKEWINEPV
DSPATQCFGK CVLVRTGLYD PVAQKFDASV IQEQFKAYPS LGEKSKVEAY ANAVQQLPST
NNDCAAVFKA YDPVHKAHKD TSKNLFHGNK ELTKGLYEKL GKDIRQKKQS YFEFCENKYY
PAGSDKRQQL CKIRQYTVLD DALFKEHTDC VMKGIRYITK NNELDAEEVK RDFMQVNKDT
KALEKVLNDC KSKEPSNAGE KSWHYYKCLV ESSVKDDFKE AFDYREVRSQ IYAFNLPKKQ
VYSKPAVQSQ VMEIDGKQCP Q
