GATC_MOUSE
ID GATC_MOUSE Reviewed; 155 AA.
AC Q8CBY0; Q8VC59; Q9D8F4;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03149};
DE Short=Glu-AdT subunit C {ECO:0000255|HAMAP-Rule:MF_03149};
DE EC=6.3.5.- {ECO:0000255|HAMAP-Rule:MF_03149};
DE Flags: Precursor;
GN Name=Gatc;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Diencephalon, and Small intestine;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Allows the formation of correctly charged Gln-tRNA(Gln)
CC through the transamidation of misacylated Glu-tRNA(Gln) in the
CC mitochondria. The reaction takes place in the presence of glutamine and
CC ATP through an activated gamma-phospho-Glu-tRNA(Gln).
CC {ECO:0000255|HAMAP-Rule:MF_03149}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) +
CC L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate;
CC Xref=Rhea:RHEA:17521, Rhea:RHEA-COMP:9681, Rhea:RHEA-COMP:9684,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359,
CC ChEBI:CHEBI:78520, ChEBI:CHEBI:78521, ChEBI:CHEBI:456216;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_03149};
CC -!- SUBUNIT: Subunit of the heterotrimeric GatCAB amidotransferase (AdT)
CC complex, composed of A (QRSL1), B (GATB) and C (GATC) subunits.
CC {ECO:0000255|HAMAP-Rule:MF_03149}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03149}.
CC -!- SIMILARITY: Belongs to the GatC family. {ECO:0000255|HAMAP-
CC Rule:MF_03149}.
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DR EMBL; AK008080; BAB25446.1; -; mRNA.
DR EMBL; AK034326; BAC28675.1; -; mRNA.
DR EMBL; BC021775; AAH21775.1; -; mRNA.
DR CCDS; CCDS19588.1; -.
DR RefSeq; NP_083921.1; NM_029645.3.
DR AlphaFoldDB; Q8CBY0; -.
DR SMR; Q8CBY0; -.
DR BioGRID; 239110; 2.
DR STRING; 10090.ENSMUSP00000115090; -.
DR iPTMnet; Q8CBY0; -.
DR PhosphoSitePlus; Q8CBY0; -.
DR EPD; Q8CBY0; -.
DR MaxQB; Q8CBY0; -.
DR PaxDb; Q8CBY0; -.
DR PeptideAtlas; Q8CBY0; -.
DR PRIDE; Q8CBY0; -.
DR ProteomicsDB; 271672; -.
DR Antibodypedia; 56388; 57 antibodies from 18 providers.
DR DNASU; 384281; -.
DR Ensembl; ENSMUST00000139167; ENSMUSP00000115090; ENSMUSG00000029536.
DR GeneID; 384281; -.
DR KEGG; mmu:384281; -.
DR UCSC; uc008zdr.1; mouse.
DR CTD; 283459; -.
DR MGI; MGI:1923776; Gatc.
DR VEuPathDB; HostDB:ENSMUSG00000029536; -.
DR eggNOG; KOG4247; Eukaryota.
DR GeneTree; ENSGT00390000018351; -.
DR HOGENOM; CLU_105899_0_2_1; -.
DR InParanoid; Q8CBY0; -.
DR OMA; CTEELLH; -.
DR OrthoDB; 1496962at2759; -.
DR PhylomeDB; Q8CBY0; -.
DR TreeFam; TF106133; -.
DR BioGRID-ORCS; 384281; 21 hits in 74 CRISPR screens.
DR ChiTaRS; Gatc; mouse.
DR PRO; PR:Q8CBY0; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8CBY0; protein.
DR Bgee; ENSMUSG00000029536; Expressed in retinal neural layer and 226 other tissues.
DR Genevisible; Q8CBY0; MM.
DR GO; GO:0030956; C:glutamyl-tRNA(Gln) amidotransferase complex; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; IDA:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0050567; F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity; ISO:MGI.
DR GO; GO:0070681; P:glutaminyl-tRNAGln biosynthesis via transamidation; ISO:MGI.
DR GO; GO:0032543; P:mitochondrial translation; ISO:MGI.
DR GO; GO:0006450; P:regulation of translational fidelity; IEA:InterPro.
DR HAMAP; MF_00122; GatC; 1.
DR InterPro; IPR003837; Asp/Glu-ADT_csu.
DR InterPro; IPR036113; Asp/Glu-ADT_sf_sub_c.
DR PANTHER; PTHR15004; PTHR15004; 1.
DR Pfam; PF02686; Glu-tRNAGln; 1.
DR SUPFAM; SSF141000; SSF141000; 1.
DR TIGRFAMs; TIGR00135; gatC; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Ligase; Mitochondrion; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome; Transit peptide.
FT TRANSIT 1..20
FT /note="Mitochondrion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03149"
FT CHAIN 21..155
FT /note="Glutamyl-tRNA(Gln) amidotransferase subunit C,
FT mitochondrial"
FT /id="PRO_0000290035"
FT CONFLICT 74
FT /note="R -> G (in Ref. 2; AAH21775)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="E -> K (in Ref. 1; BAB25446)"
FT /evidence="ECO:0000305"
FT CONFLICT 114
FT /note="E -> K (in Ref. 1; BAB25446)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="E -> K (in Ref. 1; BAB25446)"
FT /evidence="ECO:0000305"
FT CONFLICT 154
FT /note="A -> V (in Ref. 2; AAH21775)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 155 AA; 16667 MW; 2186A0EB8A084B38 CRC64;
MWSRAASVRF RAPLDAGRSF ASKANPQGKV QAAGLGTQAP RLVPQGSGRV SPAVIEHLER
LALVNFGSRE AVDRLEKAIA FADQLHAVDT DGVEPLESVL EDRCLYLRSD NVAEGSCAEE
LLQNSNHVVE EYFVAPPGNI SLPDMVNKIP SSTAE
