ALL2_DERFA
ID ALL2_DERFA Reviewed; 146 AA.
AC Q00855; P39672; Q26359;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Mite group 2 allergen Der f 2;
DE AltName: Full=Allergen Der f II;
DE AltName: Allergen=Der f 2;
DE Flags: Precursor;
GN Name=DERF2;
OS Dermatophagoides farinae (American house dust mite).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Acariformes; Sarcoptiformes; Astigmata; Psoroptidia; Analgoidea;
OC Pyroglyphidae; Dermatophagoidinae; Dermatophagoides.
OX NCBI_TaxID=6954;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX PubMed=1368682; DOI=10.1271/bbb1961.55.1233;
RA Yuuki T., Okumura Y., Ando T., Yamakawa H., Suko M., Haida M., Okudaira H.;
RT "Cloning and expression of cDNA coding for the major house dust mite
RT allergen Der f II in Escherichia coli.";
RL Agric. Biol. Chem. 55:1233-1238(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-146.
RX PubMed=8198452;
RA Okuhira H.;
RT "Molecular biology of mite antigens.";
RL Arerugi 43:435-440(1994).
RN [3]
RP DISULFIDE BONDS, AND PARTIAL PROTEIN SEQUENCE.
RX PubMed=8508052; DOI=10.1159/000236514;
RA Nishiyama C., Yuuki T., Takai T., Okumura Y., Okudaira H.;
RT "Determination of three disulfide bonds in a major house dust mite
RT allergen, Der f II.";
RL Int. Arch. Allergy Immunol. 101:159-166(1993).
RN [4]
RP PROTEIN SEQUENCE OF 18-52.
RX PubMed=2732406; DOI=10.1016/0091-6749(89)90447-8;
RA Heymann P.W., Chapman M.D., Aalberse R.C., Fox J.W., Platts-Mills T.A.E.;
RT "Antigenic and structural analysis of group II allergens (Der f II and Der
RT p II) from house dust mites (Dermatophagoides spp).";
RL J. Allergy Clin. Immunol. 83:1055-1067(1989).
RN [5]
RP STRUCTURE BY NMR.
RX PubMed=9417088; DOI=10.1074/jbc.273.1.356;
RA Ichikawa S., Hatanaka H., Yuuki T., Iwamoto N., Kojima S., Nishiyama C.,
RA Ogura K., Okumura Y., Inagaki F.;
RT "Solution structure of Der f 2, the major mite allergen for atopic
RT diseases.";
RL J. Biol. Chem. 273:356-360(1998).
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALLERGEN: Causes an allergic reaction in human. Common symptoms of mite
CC allergy are bronchial asthma, allergic rhinitis and conjunctivitis.
CC -!- MISCELLANEOUS: The sequence shown here is from clone 2. The N-terminal
CC sequence (AA 1-8) from clone 1 and 11 are not yet known.
CC -!- SIMILARITY: Belongs to the NPC2 family. {ECO:0000305}.
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DR EMBL; D10447; BAA01239.1; -; mRNA.
DR EMBL; D10448; BAA01240.1; -; mRNA.
DR EMBL; D10449; BAA01241.1; -; mRNA.
DR EMBL; S70378; AAB30829.1; -; mRNA.
DR PIR; JU0394; JU0394.
DR PDB; 1AHK; NMR; -; A=18-146.
DR PDB; 1AHM; NMR; -; A=18-146.
DR PDB; 1WRF; NMR; -; A=18-146.
DR PDB; 1XWV; X-ray; 1.83 A; A/B=18-146.
DR PDB; 2F08; X-ray; 2.20 A; A/B/C/D=18-146.
DR PDBsum; 1AHK; -.
DR PDBsum; 1AHM; -.
DR PDBsum; 1WRF; -.
DR PDBsum; 1XWV; -.
DR PDBsum; 2F08; -.
DR AlphaFoldDB; Q00855; -.
DR SMR; Q00855; -.
DR Allergome; 1108; Der f 2.0103.
DR Allergome; 1294; Der f 2.0101.
DR Allergome; 1295; Der f 2.0102.
DR Allergome; 302; Der f 2.
DR EvolutionaryTrace; Q00855; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR003172; ML_dom.
DR InterPro; IPR039670; NPC2-like.
DR PANTHER; PTHR11306; PTHR11306; 1.
DR Pfam; PF02221; E1_DerP2_DerF2; 1.
DR SMART; SM00737; ML; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Direct protein sequencing; Disulfide bond;
KW Secreted; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000269|PubMed:2732406"
FT CHAIN 18..146
FT /note="Mite group 2 allergen Der f 2"
FT /id="PRO_0000019860"
FT DISULFID 25..136
FT /evidence="ECO:0000269|PubMed:8508052"
FT DISULFID 38..44
FT /evidence="ECO:0000269|PubMed:8508052"
FT DISULFID 90..95
FT /evidence="ECO:0000269|PubMed:8508052"
FT VARIANT 93
FT /note="M -> V (in clone 1)"
FT VARIANT 105
FT /note="I -> A (in clone 11)"
FT VARIANT 128
FT /note="I -> V (in clone 11)"
FT VARIANT 142
FT /note="G -> A (in clone 11)"
FT CONFLICT 5..8
FT /note="ILCL -> GTMV (in Ref. 2; AAB30829)"
FT /evidence="ECO:0000305"
FT STRAND 23..28
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 30..35
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 36..38
FT /evidence="ECO:0007829|PDB:1AHK"
FT STRAND 40..43
FT /evidence="ECO:0007829|PDB:1AHM"
FT STRAND 44..47
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 50..59
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 68..75
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 78..80
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 83..85
FT /evidence="ECO:0007829|PDB:2F08"
FT HELIX 89..91
FT /evidence="ECO:0007829|PDB:1XWV"
FT TURN 92..94
FT /evidence="ECO:0007829|PDB:1WRF"
FT STRAND 95..97
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 102..110
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 121..129
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 132..139
FT /evidence="ECO:0007829|PDB:1XWV"
FT STRAND 142..145
FT /evidence="ECO:0007829|PDB:1XWV"
SQ SEQUENCE 146 AA; 15802 MW; FA118206CD88534A CRC64;
MISKILCLSL LVAAVVADQV DVKDCANNEI KKVMVDGCHG SDPCIIHRGK PFTLEALFDA
NQNTKTAKIE IKASLDGLEI DVPGIDTNAC HFMKCPLVKG QQYDIKYTWN VPKIAPKSEN
VVVTVKLIGD NGVLACAIAT HGKIRD
