ID   3NO4H_BUNMU             Reviewed;          87 AA.
AC   P15818; Q9YGI9;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Long neurotoxin homolog {ECO:0000305};
DE   AltName: Full=BM10-1 {ECO:0000303|PubMed:14559085};
DE   Flags: Precursor;
OS   Bungarus multicinctus (Many-banded krait).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Bungarinae; Bungarus.
OX   NCBI_TaxID=8616;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=2315016; DOI=10.1093/nar/18.4.1045;
RA   Danse J.-M., Garnier J.-M., Kempf J.;
RT   "A cDNA sequence encoding a neurotoxin-homolog from Bungarus
RT   multicinctus.";
RL   Nucleic Acids Res. 18:1045-1045(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=9844743; DOI=10.1080/15216549800204362;
RA   Qian Y.-C., Fan C.-Y., Gong Y., Yang S.-L.;
RT   "cDNA cloning and sequence analysis of six neurotoxin-like proteins from
RT   Chinese continental banded krait.";
RL   Biochem. Mol. Biol. Int. 46:821-828(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-87, FUNCTION,
RP   MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Liver, and Venom;
RX   PubMed=14559085; DOI=10.1016/s0041-0101(03)00151-x;
RA   Chang L.-S., Chung C., Liou J.-C., Chang C.-W., Yang C.-C.;
RT   "Novel neurotoxins from Taiwan banded krait (Bungarus multicinctus) venom:
RT   purification, characterization and gene organization.";
RL   Toxicon 42:323-330(2003).
CC   -!- FUNCTION: Inhibits carbachol-induced muscle contraction in a reversible
CC       manner. {ECO:0000269|PubMed:14559085}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:14559085}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7357; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:14559085};
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Ancestral
CC       subfamily. Orphan group IV sub-subfamily. {ECO:0000305}.
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DR   EMBL; X51414; CAA35776.1; -; mRNA.
DR   EMBL; AJ006136; CAA06886.1; -; mRNA.
DR   EMBL; AJ515363; CAD56380.1; -; Genomic_DNA.
DR   PIR; S08401; S08401.
DR   AlphaFoldDB; P15818; -.
DR   BMRB; P15818; -.
DR   SMR; P15818; -.
DR   PRIDE; P15818; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   InterPro; IPR035076; Toxin/TOLIP.
DR   Pfam; PF00087; Toxin_TOLIP; 1.
DR   SUPFAM; SSF57302; SSF57302; 1.
DR   PROSITE; PS00272; SNAKE_TOXIN; 1.
PE   1: Evidence at protein level;
KW   Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:14559085"
FT   CHAIN           22..87
FT                   /note="Long neurotoxin homolog"
FT                   /evidence="ECO:0000269|PubMed:14559085"
FT                   /id="PRO_0000035416"
FT   DISULFID        24..47
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        27..32
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        40..64
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        68..80
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        81..86
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   CONFLICT        77
FT                   /note="Y -> D (in Ref. 2; CAA06886)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   87 AA;  9658 MW;  57D07EC1223B4B55 CRC64;
     MKTLLLTLVV VTIVCLDLGY TMKCKICHFD TCRAGELKVC ASGEKYCFKE SWREARGTRI
     ERGCAATCPK GSVYGLYVLC CTTDDCN