ALL5_BLOTA
ID ALL5_BLOTA Reviewed; 134 AA.
AC O96870; A7IZF6; A7IZF7; A7IZG4; Q17283;
DT 20-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Mite allergen Blo t 5;
DE AltName: Allergen=Blo t 5 {ECO:0000303|PubMed:9001334};
DE Flags: Precursor;
GN Name=BLOT5;
OS Blomia tropicalis (Mite).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Acariformes; Sarcoptiformes; Astigmata; Glycyphagoidea; Echimyopodidae;
OC Blomia.
OX NCBI_TaxID=40697;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALLERGEN.
RX PubMed=9001334; DOI=10.1164/ajrccm.155.1.9001334;
RA Arruda L.K., Vailes L.D., Platts-Mills T.A.E., Fernandez-Caldas E.,
RA Montealegre F., Lin K.-L., Chua K.-Y., Rizzo M.C., Naspitz C.K.,
RA Chapman M.D.;
RT "Sensitization to Blomia tropicalis in patients with asthma and
RT identification of allergen Blo t 5.";
RL Am. J. Respir. Crit. Care Med. 155:343-350(1997).
RN [2] {ECO:0000312|EMBL:ABH06351.1, ECO:0000312|EMBL:ABH06352.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBUNIT, TISSUE SPECIFICITY, ALLERGEN,
RP AND CIRCULAR DICHROISM ANALYSIS.
RX PubMed=17445876; DOI=10.1016/j.jaci.2007.02.032;
RA Gao Y.F., Wang D.Y., Ong T.C., Tay S.L., Yap K.H., Chew F.T.;
RT "Identification and characterization of a novel allergen from Blomia
RT tropicalis: Blo t 21.";
RL J. Allergy Clin. Immunol. 120:105-112(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 63-134, AND ALLERGEN.
RX PubMed=8828535; DOI=10.1016/s0091-6749(96)70091-x;
RA Caraballo L., Avjioglu A., Marrugo J., Puerta L., Marsh D.;
RT "Cloning and expression of complementary DNA coding for an allergen with
RT common antibody-binding specificities with three allergens of the house
RT dust mite Blomia tropicalis.";
RL J. Allergy Clin. Immunol. 98:573-579(1996).
RN [4]
RP ALLERGEN.
RX PubMed=23445659; DOI=10.1186/1471-2172-14-11;
RA dos Anjos Carvalho K., de Melo-Neto O.P., Magalhaes F.B., Ponte J.C.,
RA Felipe F.A., dos Santos M.C., dos Santos Lima G., Cruz A.A., Pinheiro C.S.,
RA Pontes-de-Carvalho L.C., Alcantara-Neves N.M.;
RT "Blomia tropicalis Blo t 5 and Blo t 21 recombinant allergens might confer
RT higher specificity to serodiagnostic assays than whole mite extract.";
RL BMC Immunol. 14:11-11(2013).
RN [5] {ECO:0000312|PDB:2JMH}
RP STRUCTURE BY NMR OF 18-134, AND COILED-COIL DOMAIN.
RX PubMed=18184590; DOI=10.1016/j.str.2007.10.022;
RA Naik M.T., Chang C.-F., Kuo I.-C., Kung C.C., Yi F.-C., Chua K.Y.,
RA Huang T.-H.;
RT "Roles of structure and structural dynamics in the antibody recognition of
RT the allergen proteins: an NMR study on Blomia tropicalis major allergen.";
RL Structure 16:125-136(2008).
CC -!- SUBUNIT: May exist as homodimer and homotrimer.
CC {ECO:0000269|PubMed:17445876}.
CC -!- TISSUE SPECIFICITY: Midgut and hindgut contents as well as fecal
CC pellets (at protein level). {ECO:0000269|PubMed:17445876}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE. Common
CC symptoms of mite allergy are bronchial asthma, allergic rhinitis and
CC conjunctivitis. {ECO:0000269|PubMed:17445876,
CC ECO:0000269|PubMed:23445659, ECO:0000269|PubMed:8828535,
CC ECO:0000269|PubMed:9001334}.
CC -!- SIMILARITY: Belongs to the mite group 5 allergen family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB49396.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U59102; AAD10850.1; -; mRNA.
DR EMBL; DQ788691; ABH06358.1; -; Genomic_DNA.
DR EMBL; DQ788684; ABH06351.1; -; Genomic_DNA.
DR EMBL; DQ788685; ABH06352.1; -; Genomic_DNA.
DR EMBL; DQ788686; ABH06353.1; -; Genomic_DNA.
DR EMBL; DQ788687; ABH06354.1; -; Genomic_DNA.
DR EMBL; DQ788688; ABH06355.1; -; Genomic_DNA.
DR EMBL; DQ788689; ABH06356.1; -; Genomic_DNA.
DR EMBL; DQ788690; ABH06357.1; -; Genomic_DNA.
DR EMBL; DQ788692; ABH06359.1; -; Genomic_DNA.
DR EMBL; U27702; AAB49396.1; ALT_FRAME; Genomic_DNA.
DR PDB; 2JMH; NMR; -; A=18-134.
DR PDB; 2JRK; NMR; -; A=23-134.
DR PDB; 2MEY; NMR; -; A=18-134.
DR PDBsum; 2JMH; -.
DR PDBsum; 2JRK; -.
DR PDBsum; 2MEY; -.
DR AlphaFoldDB; O96870; -.
DR BMRB; O96870; -.
DR SMR; O96870; -.
DR Allergome; 152; Blo t 5.
DR Allergome; 3155; Blo t 5.0101.
DR Allergome; 3568; Blo t 21.
DR EvolutionaryTrace; O96870; -.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0070207; P:protein homotrimerization; IDA:UniProtKB.
DR Gene3D; 1.20.58.970; -; 1.
DR InterPro; IPR020306; Mite_allergen_group-5/21.
DR InterPro; IPR038455; Mite_allergen_group-5/21_sf.
DR Pfam; PF11642; Blo-t-5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Coiled coil; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..134
FT /note="Mite allergen Blo t 5"
FT /evidence="ECO:0000255"
FT /id="PRO_0000151065"
FT COILED 18..113
FT /evidence="ECO:0000269|PubMed:18184590"
FT CONFLICT 19
FT /note="E -> G (in Ref. 2; ABH06352)"
FT /evidence="ECO:0000305"
FT CONFLICT 23
FT /note="K -> E (in Ref. 2; ABH06359)"
FT /evidence="ECO:0000305"
FT CONFLICT 126
FT /note="Q -> P (in Ref. 3; AAB49396)"
FT /evidence="ECO:0000305"
FT HELIX 35..63
FT /evidence="ECO:0007829|PDB:2JMH"
FT HELIX 66..94
FT /evidence="ECO:0007829|PDB:2JMH"
FT HELIX 101..130
FT /evidence="ECO:0007829|PDB:2JMH"
SQ SEQUENCE 134 AA; 15642 MW; F26D763475C855FD CRC64;
MKFAIVLIAC FAASVLAQEH KPKKDDFRNE FDHLLIEQAN HAIEKGEHQL LYLQHQLDEL
NENKSKELQE KIIRELDVVC AMIEGAQGAL ERELKRTDLN ILERFNYEEA QTLSKILLKD
LKETEQKVKD IQTQ
