ALL5_DERPT
ID ALL5_DERPT Reviewed; 132 AA.
AC P14004;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Mite allergen Der p 5 {ECO:0000305};
DE AltName: Full=Allergen Der p V {ECO:0000303|PubMed:7798547};
DE AltName: Full=IgE-binding allergen {ECO:0000303|PubMed:2794865};
DE AltName: Allergen=Der p 5 {ECO:0000303|PubMed:18445190, ECO:0000303|PubMed:20534590, ECO:0000303|PubMed:24874917, ECO:0000303|PubMed:29319884};
GN Name=DERP5;
OS Dermatophagoides pteronyssinus (European house dust mite).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Acariformes; Sarcoptiformes; Astigmata; Psoroptidia; Analgoidea;
OC Pyroglyphidae; Dermatophagoidinae; Dermatophagoides.
OX NCBI_TaxID=6956;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALLERGEN.
RX PubMed=7798547; DOI=10.1016/0091-6749(94)90117-1;
RA Lin K.L., Hsieh K.H., Thomas W.R., Chiang B.L., Chua K.Y.;
RT "Characterization of Der p V allergen, cDNA analysis, and IgE-mediated
RT reactivity to the recombinant protein.";
RL J. Allergy Clin. Immunol. 94:989-996(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALLERGEN.
RX PubMed=2794865; DOI=10.1084/jem.170.4.1457;
RA Tovey E.R., Johnson M.C., Roche A.L., Cobon G.S., Baldo B.A.;
RT "Cloning and sequencing of a cDNA expressing a recombinant house dust mite
RT protein that binds human IgE and corresponds to an important low molecular
RT weight allergen.";
RL J. Exp. Med. 170:1457-1462(1989).
RN [3]
RP SEQUENCE REVISION TO 132.
RA Cobon G.S.;
RL Submitted (FEB-1990) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP ALLERGEN.
RX PubMed=18445190; DOI=10.1111/j.1398-9995.2008.01647.x;
RA Weghofer M., Dall'Antonia Y., Grote M., Stocklinger A., Kneidinger M.,
RA Balic N., Krauth M.T., Fernandez-Caldas E., Thomas W.R., van Hage M.,
RA Vieths S., Spitzauer S., Horak F., Svergun D.I., Konarev P.V., Valent P.,
RA Thalhamer J., Keller W., Valenta R., Vrtala S.;
RT "Characterization of Der p 21, a new important allergen derived from the
RT gut of house dust mites.";
RL Allergy 63:758-767(2008).
RN [5]
RP ALLERGEN.
RX PubMed=24874917; DOI=10.1016/j.pep.2014.05.001;
RA Pulsawat P., Theeraapisakkun M., Nony E., Le Mignon M., Jain K.,
RA Buaklin A., Wongpiyabovorn J., Ruxrungtham K., Jacquet A.;
RT "Characterization of the house dust mite allergen Der p 21 produced in
RT Pichia pastoris.";
RL Protein Expr. Purif. 101:8-13(2014).
RN [6]
RP ALLERGEN, BIOTECHNOLOGY, REGIONS, AND 3D-STRUCTURE MODELING.
RX PubMed=29319884; DOI=10.1111/all.13398;
RA Curin M., Garmatiuk T., Resch-Marat Y., Chen K.W., Hofer G., Fauland K.,
RA Keller W., Hemmer W., Vrtala S., Focke-Tejkl M., Valenta R.;
RT "Similar localization of conformational IgE epitopes on the house dust mite
RT allergens Der p 5 and Der p 21 despite limited IgE cross-reactivity.";
RL Allergy 73:1653-1661(2018).
RN [7] {ECO:0007744|PDB:3MQ1}
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 34-132, SUBUNIT, AND ALLERGEN.
RX PubMed=20534590; DOI=10.1074/jbc.m110.128306;
RA Mueller G.A., Gosavi R.A., Krahn J.M., Edwards L.L., Cuneo M.J.,
RA Glesner J., Pomes A., Chapman M.D., London R.E., Pedersen L.C.;
RT "Der p 5 crystal structure provides insight into the group 5 dust mite
RT allergens.";
RL J. Biol. Chem. 285:25394-25401(2010).
CC -!- SUBUNIT: Monomer. Trimer of homodimers. Oligomerizes in a
CC concentration-dependent manner. {ECO:0000269|PubMed:20534590}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Common symptoms of mite
CC allergy are bronchial asthma, allergic rhinitis and conjunctivitis.
CC Binds to IgE in patients allergic to house dust mite (HDM)
CC (PubMed:7798547, PubMed:2794865, PubMed:18445190, PubMed:24874917,
CC PubMed:29319884, PubMed:20534590). Recombinant protein binds to IgE in
CC 52% of the 25 patients tested (PubMed:7798547). Recombinant protein
CC binds to IgE in 31% of the 117 patients tested living in urban area in
CC Austria allergic to European HDM (PubMed:18445190). Recombinant protein
CC binds to IgE in 69% of the 96 patients tested living in Bangkok
CC allergic to European HDM (PubMed:24874917). Recombinant protein of 34-
CC 132 binds to IgE in 35% of the 62 patients tested (PubMed:20534590). In
CC the skin tests, the recombinant protein produces positive reactions in
CC 67% of the 15 patients tested with asthma and allergic rhinitis, in 58%
CC of the 13 patients tested with asthma alone and in 29% of the 17
CC patients tested with allergic rhinitis alone (PubMed:7798547). No
CC relevant cross-reactivity with Der p 21 allergen (PubMed:29319884).
CC Causes histamine release from human peripheral blood mononuclear
CC leukocytes. Up-regulates expression of CD203c activation marker on
CC basophils (PubMed:18445190). {ECO:0000269|PubMed:18445190,
CC ECO:0000269|PubMed:20534590, ECO:0000269|PubMed:24874917,
CC ECO:0000269|PubMed:2794865, ECO:0000269|PubMed:29319884,
CC ECO:0000269|PubMed:7798547}.
CC -!- BIOTECHNOLOGY: Non-allergenic peptides identified from the mapped major
CC conformational IgE epitope-containing areas could be used for the
CC engineering of house dust mite allergy vaccine.
CC {ECO:0000305|PubMed:29319884}.
CC -!- SIMILARITY: Belongs to the mite group 5 allergen family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA35692.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; S76337; AAB32841.1; -; mRNA.
DR EMBL; S76340; AAB32842.1; -; mRNA.
DR EMBL; X17699; CAA35692.1; ALT_INIT; mRNA.
DR PIR; S06734; S06734.
DR PDB; 3MQ1; X-ray; 2.80 A; A/B/C/D/E/F=34-132.
DR PDBsum; 3MQ1; -.
DR AlphaFoldDB; P14004; -.
DR SMR; P14004; -.
DR Allergome; 319; Der p 5.
DR Allergome; 3265; Der p 5.0101.
DR Allergome; 3266; Der p 5.0102.
DR EvolutionaryTrace; P14004; -.
DR Proteomes; UP000515146; Unplaced.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0043621; F:protein self-association; IDA:UniProtKB.
DR GO; GO:0051259; P:protein complex oligomerization; IDA:UniProtKB.
DR Gene3D; 1.20.58.970; -; 1.
DR InterPro; IPR020306; Mite_allergen_group-5/21.
DR InterPro; IPR038455; Mite_allergen_group-5/21_sf.
DR Pfam; PF11642; Blo-t-5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Reference proteome.
FT CHAIN 1..132
FT /note="Mite allergen Der p 5"
FT /id="PRO_0000151066"
FT REGION 25..53
FT /note="Immunodominant conformational IgE-binding epitope"
FT /evidence="ECO:0000269|PubMed:29319884"
FT REGION 102..132
FT /note="Immunodominant conformational IgE-binding epitope"
FT /evidence="ECO:0000269|PubMed:29319884"
FT VARIANT 61
FT /note="E -> A"
FT HELIX 35..61
FT /evidence="ECO:0007829|PDB:3MQ1"
FT HELIX 65..93
FT /evidence="ECO:0007829|PDB:3MQ1"
FT HELIX 100..128
FT /evidence="ECO:0007829|PDB:3MQ1"
SQ SEQUENCE 132 AA; 15615 MW; 3C840C3C26707C05 CRC64;
MKFIIAFFVA TLAVMTVSGE DKKHDYQNEF DFLLMERIHE QIKKGELALF YLQEQINHFE
EKPTKEMKDK IVAEMDTIIA MIDGVRGVLD RLMQRKDLDI FEQYNLEMAK KSGDILERDL
KKEEARVKKI EV
