GATLA_ARATH
ID GATLA_ARATH Reviewed; 365 AA.
AC Q9LHD2;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Probable galacturonosyltransferase-like 10;
DE EC=2.4.1.-;
DE AltName: Full=Galactinol synthase 8;
DE Short=AtGolS8;
DE Short=GolS-8;
GN Name=GATL10; Synonyms=GOLS8; OrderedLocusNames=At3g28340;
GN ORFNames=MZF16.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Cheuk R., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16540543; DOI=10.1073/pnas.0600120103;
RA Sterling J.D., Atmodjo M.A., Inwood S.E., Kumar Kolli V.S., Quigley H.F.,
RA Hahn M.G., Mohnen D.;
RT "Functional identification of an Arabidopsis pectin biosynthetic
RT homogalacturonan galacturonosyltransferase.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:5236-5241(2006).
RN [6]
RP INDUCTION BY METHYLVIOLOGEN, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18502973; DOI=10.1104/pp.108.122465;
RA Nishizawa A., Yabuta Y., Shigeoka S.;
RT "Galactinol and raffinose constitute a novel function to protect plants
RT from oxidative damage.";
RL Plant Physiol. 147:1251-1263(2008).
CC -!- FUNCTION: May be involved in pectin and/or xylans biosynthesis in cell
CC walls. {ECO:0000250}.
CC -!- PATHWAY: Glycan metabolism; pectin biosynthesis.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC pass type II membrane protein {ECO:0000250}.
CC -!- INDUCTION: By methylviologen (MV), a superoxide radical generating
CC drug. {ECO:0000269|PubMed:18502973}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family. {ECO:0000305}.
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DR EMBL; AP002051; BAB02626.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77435.1; -; Genomic_DNA.
DR EMBL; BT011750; AAS49113.1; -; mRNA.
DR EMBL; AK176597; BAD44360.1; -; mRNA.
DR RefSeq; NP_189474.2; NM_113753.5.
DR AlphaFoldDB; Q9LHD2; -.
DR SMR; Q9LHD2; -.
DR BioGRID; 7791; 1.
DR STRING; 3702.AT3G28340.1; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR PaxDb; Q9LHD2; -.
DR PRIDE; Q9LHD2; -.
DR ProteomicsDB; 230450; -.
DR EnsemblPlants; AT3G28340.1; AT3G28340.1; AT3G28340.
DR GeneID; 822462; -.
DR Gramene; AT3G28340.1; AT3G28340.1; AT3G28340.
DR KEGG; ath:AT3G28340; -.
DR Araport; AT3G28340; -.
DR TAIR; locus:2095420; AT3G28340.
DR eggNOG; ENOG502QTN8; Eukaryota.
DR HOGENOM; CLU_034713_0_0_1; -.
DR InParanoid; Q9LHD2; -.
DR OMA; NIYFHFI; -.
DR OrthoDB; 1520602at2759; -.
DR PhylomeDB; Q9LHD2; -.
DR UniPathway; UPA00845; -.
DR PRO; PR:Q9LHD2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LHD2; baseline and differential.
DR Genevisible; Q9LHD2; AT.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0047262; F:polygalacturonate 4-alpha-galacturonosyltransferase activity; ISS:TAIR.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0045489; P:pectin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006979; P:response to oxidative stress; IEP:TAIR.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR002495; Glyco_trans_8.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF01501; Glyco_transf_8; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Glycoprotein; Glycosyltransferase;
KW Golgi apparatus; Membrane; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..365
FT /note="Probable galacturonosyltransferase-like 10"
FT /id="PRO_0000392612"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..365
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 209
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 365 AA; 41177 MW; EA911FC3DACD46B1 CRC64;
MMSGSRLASR LIIIFSIIST SFFTVESIRL FPDSFDDASS DLMEAPAYQN GLDCSVLAKN
RLLLACDPSA VHIAMTLDPA YLRGTVSAVH SILKHTSCPE NIFFHFIASG TSQGSLAKTL
SSVFPSLSFK VYTFEETTVK NLISSSIRQA LDSPLNYARS YLSEILSSCV SRVIYLDSDV
IVVDDIQKLW KISLSGSRTI GAPEYCHANF TKYFTDSFWS DQKLSSVFDS KTPCYFNTGV
MVIDLERWRE GDYTRKIENW MKIQKEDKRI YELGSLPPFL LVFGGDIEAI DHQWNQHGLG
GDNIVSSCRS LHPGPVSLIH WSGKGKPWVR LDDGKPCPID YLWAPYDLHK SQRQYLQYNQ
ELEIL