ALL6_APIME
ID ALL6_APIME Reviewed; 92 AA.
AC P83563; Q27SJ7; Q27SJ8;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2019, sequence version 2.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Allergen Api m 6.03 / Api m 6.04 {ECO:0000303|PubMed:11344362, ECO:0000303|PubMed:17069633};
DE AltName: Allergen=Api m 6 {ECO:0000303|PubMed:11344362};
DE Contains:
DE RecName: Full=Allergen Api m 6.01 / Api m 6.02 {ECO:0000303|PubMed:11344362, ECO:0000303|PubMed:17069633};
DE Flags: Precursor;
OS Apis mellifera (Honeybee).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC Apis.
OX NCBI_TaxID=7460;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (API M 6.03 AND API M 6.04).
RC TISSUE=Venom gland;
RX PubMed=17069633; DOI=10.1111/j.1365-2583.2006.00669.x;
RA Peiren N., de Graaf D.C., Evans J.D., Jacobs F.J.;
RT "Genomic and transcriptional analysis of protein heterogeneity of the
RT honeybee venom allergen Api m 6.";
RL Insect Mol. Biol. 15:577-581(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (API M 6.03 AND API M 6.04).
RC STRAIN=DH4;
RA Wu J.L., Liu J.H., Yuan Y.N., Qiao L.Y., Liu W.Z.;
RL Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 22-92 (API M 6.01; API M 6.02; API M 6.03 AND API M
RP 6.04), MASS SPECTROMETRY, ALLERGEN, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=11344362; DOI=10.1067/mai.2001.113867;
RA Kettner A., Hughes G.J., Frutiger S., Astori M., Roggero M., Spertini F.,
RA Corradin G.;
RT "Api m 6: a new bee venom allergen.";
RL J. Allergy Clin. Immunol. 107:914-920(2001).
RN [4]
RP 3D-STRUCTURE MODELING, AND ALLERGEN.
RX PubMed=23397669;
RA Michel Y., McIntyre M., Ginglinger H., Ollert M., Cifuentes L., Blank S.,
RA Spillner E.;
RT "The putative serine protease inhibitor Api m 6 from Apis mellifera venom:
RT recombinant and structural evaluation.";
RL J. Investig. Allergol. Clin. Immunol. 22:476-484(2012).
RN [5]
RP IDENTIFICATION.
RC STRAIN=DH4;
RG EnsemblMetazoa;
RL Submitted (JAN-2017) to UniProtKB.
CC -!- FUNCTION: May act as a protease inhibitor.
CC {ECO:0000305|PubMed:23397669}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11344362}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:11344362}.
CC -!- MASS SPECTROMETRY: [Allergen Api m 6.03 / Api m 6.04]: Mass=7190;
CC Method=MALDI; Note=in Api m 6.01.;
CC Evidence={ECO:0000269|PubMed:11344362};
CC -!- MASS SPECTROMETRY: [Allergen Api m 6.03 / Api m 6.04]: Mass=7400;
CC Method=MALDI; Note=in Api m 6.02.;
CC Evidence={ECO:0000269|PubMed:11344362};
CC -!- MASS SPECTROMETRY: [Allergen Api m 6.03 / Api m 6.04]: Mass=7598;
CC Method=MALDI; Note=in Api m 6.03.;
CC Evidence={ECO:0000269|PubMed:11344362};
CC -!- MASS SPECTROMETRY: [Allergen Api m 6.03 / Api m 6.04]: Mass=7808;
CC Method=MALDI; Note=in Api m 6.04.;
CC Evidence={ECO:0000269|PubMed:11344362};
CC -!- ALLERGEN: Causes an allergic reaction in human (PubMed:11344362,
CC PubMed:23397669). Binds to IgE (42% responsiveness) (PubMed:11344362,
CC PubMed:23397669). {ECO:0000269|PubMed:11344362,
CC ECO:0000269|PubMed:23397669}.
CC -!- SIMILARITY: Belongs to the serine protease inhibitor-like (TIL domain-
CC containing) family. {ECO:0000305}.
CC -!- CAUTION: Four isoforms exist; 6.01 (without 4 N-ter AA and 2 extra C-
CC ter AA), 6.02 (without 4 N-ter AA but with 2 extra C-ter AA), 6.03
CC (with 4 N-ter AA but without 2 extra C-ter AA) and 6.04 (with 4 N-ter
CC AA and with 2 extra C-ter AA) (PubMed:17069633, PubMed:11344362). The
CC sequence displayed corresponds to isoform 6.03. It is unknown if the
CC variants api m 6.01 and api m 6.02 (without 4 N-ter AA) are the result
CC of different transcript, an alternative splicing, or an enzymatic
CC cleavage. {ECO:0000305|PubMed:11344362, ECO:0000305|PubMed:17069633}.
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DR EMBL; DQ384990; ABD51778.1; -; mRNA.
DR EMBL; DQ384991; ABD51779.1; -; mRNA.
DR RefSeq; NP_001035360.1; NM_001040270.1.
DR RefSeq; XP_003252043.1; XM_003251995.3.
DR AlphaFoldDB; P83563; -.
DR SMR; P83563; -.
DR STRING; 7460.GB45615-PA; -.
DR Allergome; 3092; Api m 6.0101.
DR Allergome; 49; Api m 6.
DR PaxDb; P83563; -.
DR EnsemblMetazoa; NM_001040270; NP_001035360; LOC678674.
DR GeneID; 678674; -.
DR KEGG; ame:678674; -.
DR HOGENOM; CLU_156801_2_1_1; -.
DR OrthoDB; 1608992at2759; -.
DR Proteomes; UP000005203; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR InterPro; IPR036084; Ser_inhib-like_sf.
DR InterPro; IPR002919; TIL_dom.
DR Pfam; PF01826; TIL; 1.
DR SUPFAM; SSF57567; SSF57567; 1.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW Reference proteome; Secreted; Serine protease inhibitor; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..92
FT /note="Allergen Api m 6.03 / Api m 6.04"
FT /evidence="ECO:0000269|PubMed:11344362"
FT /id="PRO_0000174335"
FT CHAIN 26..92
FT /note="Allergen Api m 6.01 / Api m 6.02"
FT /evidence="ECO:0000269|PubMed:11344362"
FT /id="PRO_0000446186"
FT DOMAIN 37..91
FT /note="TIL"
FT /evidence="ECO:0000255"
FT SITE 65..66
FT /note="Reactive bond"
FT /evidence="ECO:0000305"
FT DISULFID 37..71
FT /evidence="ECO:0000250|UniProtKB:P07851"
FT DISULFID 46..67
FT /evidence="ECO:0000250|UniProtKB:P07851"
FT DISULFID 50..63
FT /evidence="ECO:0000250|UniProtKB:P07851"
FT DISULFID 54..91
FT /evidence="ECO:0000250|UniProtKB:P07851"
FT DISULFID 73..85
FT /evidence="ECO:0000250|UniProtKB:P07851"
FT VARIANT 92
FT /note="G -> LPG (in Api m 6.02 and Api m 6.04)"
FT CONFLICT 14
FT /note="I -> V (in Ref. 1; ABD51779)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 92 AA; 9819 MW; 850FE1338198A028 CRC64;
MSRLVLASFL LLAIFSMLVG GFGGFGGFGG LGGRGKCPSN EIFSRCDGRC QRFCPNVVPK
PLCIKICAPG CVCRLGYLRN KKKVCVPRSK CG
