GAUTA_ARATH
ID GAUTA_ARATH Reviewed; 536 AA.
AC Q9SKT6; Q67XF6; Q93VL7;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Probable galacturonosyltransferase 10;
DE EC=2.4.1.-;
DE AltName: Full=Like glycosyl transferase 4;
GN Name=GAUT10; Synonyms=LGT4; OrderedLocusNames=At2g20810; ORFNames=F5H14.44;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=10809443; DOI=10.1023/a:1006368316413;
RA Tavares R., Aubourg S., Lecharny A., Kreis M.;
RT "Organization and structural evolution of four multigene families in
RT Arabidopsis thaliana: AtLCAD, AtLGT, AtMYST and AtHD-GL2.";
RL Plant Mol. Biol. 42:703-717(2000).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16540543; DOI=10.1073/pnas.0600120103;
RA Sterling J.D., Atmodjo M.A., Inwood S.E., Kumar Kolli V.S., Quigley H.F.,
RA Hahn M.G., Mohnen D.;
RT "Functional identification of an Arabidopsis pectin biosynthetic
RT homogalacturonan galacturonosyltransferase.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:5236-5241(2006).
RN [7]
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19825675; DOI=10.1093/mp/ssp062;
RA Caffall K.H., Pattathil S., Phillips S.E., Hahn M.G., Mohnen D.;
RT "Arabidopsis thaliana T-DNA mutants implicate GAUT genes in the
RT biosynthesis of pectin and xylan in cell walls and seed testa.";
RL Mol. Plant 2:1000-1014(2009).
CC -!- FUNCTION: May be involved in pectin and/or xylans biosynthesis in cell
CC walls. {ECO:0000250}.
CC -!- PATHWAY: Glycan metabolism; pectin biosynthesis.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC pass type II membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, inflorescences, siliques,
CC leaves and stems. {ECO:0000269|PubMed:19825675}.
CC -!- DISRUPTION PHENOTYPE: No obvious phenotype. Reduced galacturonic acid
CC content in cell wall. {ECO:0000269|PubMed:19825675}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family. {ECO:0000305}.
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DR EMBL; AC006234; AAD20914.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC07076.1; -; Genomic_DNA.
DR EMBL; AY035019; AAK59524.1; -; mRNA.
DR EMBL; AY059085; AAL15191.1; -; mRNA.
DR EMBL; AK176863; BAD44626.1; -; mRNA.
DR PIR; F84593; F84593.
DR RefSeq; NP_565485.1; NM_127647.2.
DR AlphaFoldDB; Q9SKT6; -.
DR SMR; Q9SKT6; -.
DR STRING; 3702.AT2G20810.1; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR iPTMnet; Q9SKT6; -.
DR PaxDb; Q9SKT6; -.
DR PRIDE; Q9SKT6; -.
DR ProteomicsDB; 221906; -.
DR EnsemblPlants; AT2G20810.1; AT2G20810.1; AT2G20810.
DR GeneID; 816611; -.
DR Gramene; AT2G20810.1; AT2G20810.1; AT2G20810.
DR KEGG; ath:AT2G20810; -.
DR Araport; AT2G20810; -.
DR TAIR; locus:2051426; AT2G20810.
DR eggNOG; ENOG502QRMA; Eukaryota.
DR HOGENOM; CLU_010770_5_0_1; -.
DR InParanoid; Q9SKT6; -.
DR OMA; APMRAWF; -.
DR OrthoDB; 463368at2759; -.
DR PhylomeDB; Q9SKT6; -.
DR UniPathway; UPA00845; -.
DR PRO; PR:Q9SKT6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SKT6; baseline and differential.
DR Genevisible; Q9SKT6; AT.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000137; C:Golgi cis cisterna; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0047262; F:polygalacturonate 4-alpha-galacturonosyltransferase activity; ISS:TAIR.
DR GO; GO:0052546; P:cell wall pectin metabolic process; IGI:TAIR.
DR GO; GO:0010442; P:guard cell morphogenesis; IGI:TAIR.
DR GO; GO:0048358; P:mucilage pectin biosynthetic process; IMP:TAIR.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR029993; GAUT.
DR InterPro; IPR002495; Glyco_trans_8.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR32116; PTHR32116; 1.
DR Pfam; PF01501; Glyco_transf_8; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Cell wall biogenesis/degradation; Glycoprotein; Glycosyltransferase;
KW Golgi apparatus; Membrane; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..536
FT /note="Probable galacturonosyltransferase 10"
FT /id="PRO_0000392560"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..536
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 64
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 246
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 300
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 403
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 436
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 327
FT /note="G -> R (in Ref. 4; BAD44626)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 536 AA; 61812 MW; 9E9CF8FDB393179B CRC64;
MRRRGGDSFR RAGRRKISNV VWWVLSGIAL LLFFLILSKA GHIEPRPSIP KRRYRNDKFV
EGMNMTEEML SPTSVARQVN DQIALAKAFV VIAKESKNLQ FAWDLSAQIR NSQLLLSSAA
TRRSPLTVLE SESTIRDMAV LLYQAQQLHY DSATMIMRLK ASIQALEEQM SSVSEKSSKY
GQIAAEEVPK SLYCLGVRLT TEWFQNLDLQ RTLKERSRVD SKLTDNSLYH FCVFSDNIIA
TSVVVNSTAL NSKAPEKVVF HLVTNEINYA AMKAWFAINM DNLRGVTVEV QKFEDFSWLN
ASYVPVLKQL QDSDTQSYYF SGHNDDGRTP IKFRNPKYLS MLNHLRFYIP EVFPALKKVV
FLDDDVVVQK DLSSLFSIDL NKNVNGAVET CMETFHRYHK YLNYSHPLIR SHFDPDACGW
AFGMNVFDLV EWRKRNVTGI YHYWQEKNVD RTLWKLGTLP PGLLTFYGLT EALEASWHIL
GLGYTNVDAR VIEKGAVLHF NGNLKPWLKI GIEKYKPLWE RYVDYTSPFM QQCNFH
