GAUTD_ARATH
ID GAUTD_ARATH Reviewed; 533 AA.
AC Q0WV13; B3H768; Q9MAB8;
DT 23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Probable galacturonosyltransferase 13;
DE EC=2.4.1.-;
GN Name=GAUT13; OrderedLocusNames=At3g01040; ORFNames=T4P13.28;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16540543; DOI=10.1073/pnas.0600120103;
RA Sterling J.D., Atmodjo M.A., Inwood S.E., Kumar Kolli V.S., Quigley H.F.,
RA Hahn M.G., Mohnen D.;
RT "Functional identification of an Arabidopsis pectin biosynthetic
RT homogalacturonan galacturonosyltransferase.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:5236-5241(2006).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=19825675; DOI=10.1093/mp/ssp062;
RA Caffall K.H., Pattathil S., Phillips S.E., Hahn M.G., Mohnen D.;
RT "Arabidopsis thaliana T-DNA mutants implicate GAUT genes in the
RT biosynthesis of pectin and xylan in cell walls and seed testa.";
RL Mol. Plant 2:1000-1014(2009).
CC -!- FUNCTION: May be involved in pectin and/or xylans biosynthesis in cell
CC walls. {ECO:0000250}.
CC -!- PATHWAY: Glycan metabolism; pectin biosynthesis.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC pass type II membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0WV13-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0WV13-2; Sequence=VSP_038821;
CC -!- TISSUE SPECIFICITY: Expressed in roots, inflorescences, siliques,
CC leaves and stems. {ECO:0000269|PubMed:19825675}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF26170.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC008261; AAF26170.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002686; AEE73599.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63701.1; -; Genomic_DNA.
DR EMBL; AK226967; BAE99035.1; -; mRNA.
DR EMBL; BT030321; ABO09884.1; -; mRNA.
DR RefSeq; NP_001118545.1; NM_001125073.2. [Q0WV13-2]
DR RefSeq; NP_001319437.1; NM_001337305.1. [Q0WV13-2]
DR AlphaFoldDB; Q0WV13; -.
DR SMR; Q0WV13; -.
DR STRING; 3702.AT3G01040.1; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR iPTMnet; Q0WV13; -.
DR PaxDb; Q0WV13; -.
DR PRIDE; Q0WV13; -.
DR ProteomicsDB; 221908; -. [Q0WV13-1]
DR EnsemblPlants; AT3G01040.2; AT3G01040.2; AT3G01040. [Q0WV13-2]
DR EnsemblPlants; AT3G01040.3; AT3G01040.3; AT3G01040. [Q0WV13-2]
DR GeneID; 821312; -.
DR Gramene; AT3G01040.2; AT3G01040.2; AT3G01040. [Q0WV13-2]
DR Gramene; AT3G01040.3; AT3G01040.3; AT3G01040. [Q0WV13-2]
DR KEGG; ath:AT3G01040; -.
DR Araport; AT3G01040; -.
DR TAIR; locus:2102082; AT3G01040.
DR eggNOG; ENOG502QQKV; Eukaryota.
DR HOGENOM; CLU_010770_5_1_1; -.
DR InParanoid; Q0WV13; -.
DR OMA; NSNFTLW; -.
DR PhylomeDB; Q0WV13; -.
DR UniPathway; UPA00845; -.
DR PRO; PR:Q0WV13; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q0WV13; baseline and differential.
DR Genevisible; Q0WV13; AT.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0047262; F:polygalacturonate 4-alpha-galacturonosyltransferase activity; ISS:TAIR.
DR GO; GO:0052325; P:cell wall pectin biosynthetic process; IGI:TAIR.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IGI:TAIR.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR029993; GAUT.
DR InterPro; IPR002495; Glyco_trans_8.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR32116; PTHR32116; 1.
DR Pfam; PF01501; Glyco_transf_8; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell wall biogenesis/degradation; Glycoprotein;
KW Glycosyltransferase; Golgi apparatus; Membrane; Reference proteome;
KW Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..533
FT /note="Probable galacturonosyltransferase 13"
FT /id="PRO_0000392563"
FT TOPO_DOM 1..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..533
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 306
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 396
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 445
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 520
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 90
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038821"
SQ SEQUENCE 533 AA; 61144 MW; 743722DE9C4F1813 CRC64;
MQLHISPSMR SITISSSNEF IDLMKIKVAA RHISYRTLFH TILILAFLLP FVFILTAVVT
LEGVNKCSSF DCFGRRLGPR LLGRIDDSEQ RLVRDFYKIL NEVSTQEIPD GLKLPESFSQ
LVSDMKNNHY DAKTFALVFR AMVEKFERDL RESKFAELMN KHFAASSIPK GIHCLSLRLT
DEYSSNAHAR RQLPSPELLP VLSDNAYHHF VLATDNILAA SVVVSSAVQS SSKPEKIVFH
VITDKKTYAG MHSWFALNSV APAIVEVKSV HQFDWLTREN VPVLEAVESH NSIRNYYHGN
HIAGANLSET TPRTFASKLQ SRSPKYISLL NHLRIYLPEL FPNLDKVVFL DDDIVIQKDL
SPLWDIDLNG KVNGAVETCR GEDVWVMSKR LRNYFNFSHP LIAKHLDPEE CAWAYGMNIF
DLRTWRKTNI RETYHSWLKE NLKSNLTMWK LGTLPPALIA FKGHVQPIDS SWHMLGLGYQ
SKTNLENAKK AAVIHYNGQS KPWLEIGFEH LRPFWTKYVN YSNDFIKNCH ILE
