GBB1_CAEEL
ID GBB1_CAEEL Reviewed; 340 AA.
AC P17343; Q19394; Q968T1;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Guanine nucleotide-binding protein subunit beta-1;
GN Name=gpb-1; ORFNames=F13D12.7;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=2110981; DOI=10.1016/s0022-2836(05)80118-4;
RA van der Voorn L., Gebbink M., Plasterk R.H.A., Ploegh H.L.;
RT "Characterization of a G-protein beta-subunit gene from the nematode
RT Caenorhabditis elegans.";
RL J. Mol. Biol. 213:17-26(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Bristol N2;
RX PubMed=11333232; DOI=10.1093/genetics/158.1.221;
RA van Der Linden A.M., Simmer F., Cuppen E., Plasterk R.H.A.;
RT "The G protein beta subunit gpb-2 in Caenorhabditis elegans regulates the
RT G(o)alpha-G(q)alpha signaling network through interactions with the
RT regulator of G protein signaling proteins egl-10 and eat-16.";
RL Genetics 158:221-235(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=14534135; DOI=10.1242/dev.00790;
RA Tsou M.-F.B., Hayashi A., Rose L.S.;
RT "LET-99 opposes Galpha/GPR signaling to generate asymmetry for spindle
RT positioning in response to PAR and MES-1/SRC-1 signaling.";
RL Development 130:5717-5730(2003).
RN [5]
RP INTERACTION WITH GOA-1.
RX PubMed=12730122; DOI=10.1101/gad.1081203;
RA Srinivasan D.G., Fisk R.M., Xu H., van den Heuvel S.;
RT "A complex of LIN-5 and GPR proteins regulates G protein signaling and
RT spindle function in C elegans.";
RL Genes Dev. 17:1225-1239(2003).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC as a modulator or transducer in various transmembrane signaling
CC systems. The beta and gamma chains are required for the GTPase
CC activity, for replacement of GDP by GTP, and for G protein-effector
CC interaction. In the early embryo, controls the magnitude of the forces
CC acting on centrosomes but is not required for generating asymmetric
CC forces (PubMed:14534135). {ECO:0000269|PubMed:14534135, ECO:0000305}.
CC -!- SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma (By
CC similarity). Interacts with G protein gamma subunits gpc-1 and gpc-2
CC and with egl-10 and eat-16 (PubMed:11333232). Interacts with goa-1 (in
CC GDP-bound form) (PubMed:12730122). {ECO:0000250|UniProtKB:P63212,
CC ECO:0000269|PubMed:11333232, ECO:0000269|PubMed:12730122}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in one-cell embryo
CC results in hyperactive nuclear and spindle movements from early
CC prophase to metaphase characterized by vigorous rocking of
CC nuclear/centrosome complex and its failure to center properly. Normal
CC centrosome alignment during metaphase. {ECO:0000269|PubMed:14534135}.
CC -!- SIMILARITY: Belongs to the WD repeat G protein beta family.
CC {ECO:0000305}.
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DR EMBL; X17497; CAA35532.1; -; Genomic_DNA.
DR EMBL; AF291846; AAK55963.1; -; mRNA.
DR EMBL; Z49127; CAA88948.1; -; Genomic_DNA.
DR PIR; S09591; RGKWB.
DR PIR; T20830; T20830.
DR RefSeq; NP_001254312.1; NM_001267383.1.
DR AlphaFoldDB; P17343; -.
DR SMR; P17343; -.
DR BioGRID; 40108; 8.
DR IntAct; P17343; 1.
DR STRING; 6239.F13D12.7a.1; -.
DR iPTMnet; P17343; -.
DR EPD; P17343; -.
DR PaxDb; P17343; -.
DR PeptideAtlas; P17343; -.
DR EnsemblMetazoa; F13D12.7a.1; F13D12.7a.1; WBGene00001679.
DR UCSC; F13D12.7.1; c. elegans.
DR WormBase; F13D12.7a; CE02186; WBGene00001679; gpb-1.
DR eggNOG; KOG0286; Eukaryota.
DR GeneTree; ENSGT01000000214413; -.
DR InParanoid; P17343; -.
DR OMA; PLDSQWV; -.
DR OrthoDB; 704786at2759; -.
DR PhylomeDB; P17343; -.
DR Reactome; R-CEL-1296041; Activation of G protein gated Potassium channels.
DR Reactome; R-CEL-202040; G-protein activation.
DR Reactome; R-CEL-2485179; Activation of the phototransduction cascade.
DR Reactome; R-CEL-381753; Olfactory Signaling Pathway.
DR Reactome; R-CEL-392170; ADP signalling through P2Y purinoceptor 12.
DR Reactome; R-CEL-392451; G beta:gamma signalling through PI3Kgamma.
DR Reactome; R-CEL-400042; Adrenaline,noradrenaline inhibits insulin secretion.
DR Reactome; R-CEL-4086398; Ca2+ pathway.
DR Reactome; R-CEL-416476; G alpha (q) signalling events.
DR Reactome; R-CEL-416482; G alpha (12/13) signalling events.
DR Reactome; R-CEL-418555; G alpha (s) signalling events.
DR Reactome; R-CEL-418594; G alpha (i) signalling events.
DR Reactome; R-CEL-418597; G alpha (z) signalling events.
DR Reactome; R-CEL-428930; Thromboxane signalling through TP receptor.
DR Reactome; R-CEL-456926; Thrombin signalling through proteinase activated receptors (PARs).
DR Reactome; R-CEL-6814122; Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
DR Reactome; R-CEL-8964616; G beta:gamma signalling through CDC42.
DR Reactome; R-CEL-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR Reactome; R-CEL-997272; Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
DR PRO; PR:P17343; -.
DR Proteomes; UP000001940; Chromosome II.
DR ExpressionAtlas; P17343; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; ISS:WormBase.
DR GO; GO:0030159; F:signaling receptor complex adaptor activity; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IMP:WormBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0040013; P:negative regulation of locomotion; IMP:WormBase.
DR GO; GO:0046662; P:regulation of oviposition; IMP:WormBase.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR001632; Gprotein_B.
DR InterPro; IPR016346; Guanine_nucleotide-bd_bsu.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19850; PTHR19850; 1.
DR Pfam; PF00400; WD40; 6.
DR PRINTS; PR00319; GPROTEINB.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Mitosis; Reference proteome; Repeat; Transducer;
KW WD repeat.
FT CHAIN 1..340
FT /note="Guanine nucleotide-binding protein subunit beta-1"
FT /id="PRO_0000127712"
FT REPEAT 53..83
FT /note="WD 1"
FT REPEAT 95..125
FT /note="WD 2"
FT REPEAT 141..170
FT /note="WD 3"
FT REPEAT 182..212
FT /note="WD 4"
FT REPEAT 224..254
FT /note="WD 5"
FT REPEAT 268..298
FT /note="WD 6"
FT REPEAT 310..340
FT /note="WD 7"
FT CONFLICT 240
FT /note="A -> R (in Ref. 2; CAA35532)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 340 AA; 37406 MW; DF83F9C299F124BD CRC64;
MSELDQLRQE AEQLKSQIRE ARKSANDTTL ATVASNLEPI GRIQMRTRRT LRGHLAKIYA
MHWASDSRNL VSASQDGKLI VWDSYTTNKV HAIPLRSSWV MTCAYAPSGS FVACGGLDNI
CSIYSLKTRE GNVRVSRELP GHTGYLSCCR FLDDNQIVTS SGDMTCALWD IETGQQCTAF
TGHTGDVMSL SLSPDFRTFI SGACDASAKL WDIRDGMCKQ TFPGHESDIN AVAFFPSGNA
FATGSDDATC RLFDIRADQE LAMYSHDNII CGITSVAFSK SGRLLFAGYD DFNCNVWDSM
RQERAGVLAG HDNRVSCLGV TEDGMAVCTG SWDSFLKIWN
